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The caseins of buffalo milk

Published online by Cambridge University Press:  01 June 2009

F. Addeo
Affiliation:
Laboratoire de Recherches sur les Protéines, Institut National de la Recherche Agronomique, CN RZ, 78350 Jouy-en-Josas, France
J.-C. Mercier
Affiliation:
Laboratoire de Recherches sur les Protéines, Institut National de la Recherche Agronomique, CN RZ, 78350 Jouy-en-Josas, France
B. Ribadeau-Dumas
Affiliation:
Laboratoire de Recherches sur les Protéines, Institut National de la Recherche Agronomique, CN RZ, 78350 Jouy-en-Josas, France

Summary

The components of whole casein from water buffalo (Bubalis L.) have been re-investigated in the light of recent studies elucidating the primary structure of all the bovine caseins (αs1, αs2, β & κ). Whole casein samples obtained from individual Italian buffaloes all contained 2 αs1- and 2αs2-casein fractions. These 4 components were isolated and identified by their amino acid compositions and terminal amino acid sequences. The 2 αs1-casein fractions seem to have identical peptide chains as do the 2 αs2 fractions, with the individual fractions within each group differing only in their phosphate content. Buffalo β-casein was also isolated and characterized by its amino acid composition and terminal sequences. The isolation of different buffalo κ-casein fractions was reported earlier (Addeo, Chobert & Ribadeau-Dumas, 1977). These fractions were purified and analysed. They were found to have the same amino acid compositions and C-terminal sequences, but to differ in their carbohydrate content. The prominent component, κ1, was treated with chymosin; the caseinomacropeptide and para κ-casein were separated from the digest and analysed. Starchgel electrophoresis at alkaline pH showed no polymorphism of αs1-, β- and κ-caseins in 170 individual buffalo whole casein samples. On 5 occasions the electrophoretic pattern of αs2-casein suggested genetic polymorphism. The behaviour of buffalo αs1−and αs2-caseins in the presence of Ca2+ was also studied.

Type
Original Articles
Copyright
Copyright © Proprietors of Journal of Dairy Research 1977

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