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Capture immunoassay for the diagnosis of bovine mastitis using a monoclonal antibody to polymorphonuclear granulocytes

Published online by Cambridge University Press:  01 June 2009

Catherine A. O'Sullivan ,
Patrick J. Joyce ,
Teresa Sloan  and
Alan G. Shattock
Catherine A. O'Sullivan
Affiliation:
Department of Zoology and University College Dublin, Belfield, Dublin 4, Irish Republic
Patrick J. Joyce
Affiliation:
Department of Zoology and University College Dublin, Belfield, Dublin 4, Irish Republic
Teresa Sloan
Affiliation:
Department of Zoology and University College Dublin, Belfield, Dublin 4, Irish Republic
Alan G. Shattock
Affiliation:
Department of Medical Microbiology, University College Dublin, Belfield, Dublin 4, Irish Republic
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Summary

A direct capture enzyme-linked immunosorbent assay (ELISA) was developed to measure elevated polymorphonuclear granulocyte (PMN) antigens using horseradish peroxidase (EC 1.11.1.7) conjugated rabbit polyclonal anti-PMN antisera and a monoclonal antibody specific for PMN cells. Optical densities obtained in the ELISA were used to predict the cell counts of milk samples. Predicted counts were not significantly different from actual somatic cell counts (SCC). In a total of 156 bovine milk samples the correlation coefficient between somatic cell counting, taking > 500000 cells/ml as being indicative of mastitis, and the assay was 0·94, yielding an assay sensitivity of 95·2% and a specificity of 97·3%. In further trials the ELISA could detect elevated PMN antigens in milk with SCC as low as 100000 cells/ml. The results indicate that the monoclonal antibody based direct ELISA has excellent potential in the detection and determination of bovine mastitis.

Type
Original Articles
Information
Journal of Dairy Research , Volume 59 , Issue 2 , May 1992 , pp. 123 - 133
Copyright
Copyright © Proprietors of Journal of Dairy Research 1992

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