Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-27T00:26:37.248Z Has data issue: false hasContentIssue false
  • English
  • Français

Basal levels of prolactin in goat blood measured throughout a 24-h period by a rapid double antibody–solid phase radioimmunoassay

Published online by Cambridge University Press:  01 June 2009

I. C. Hart
I. C. Hart
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading, RG2 9AT
Get access Rights & Permissions [Opens in a new window]

Summary

The development of a rapid, highly sensitive double antibody–solid phase (DASP) radioimmunoassay for ovine/caprine prolactin is described. The assay was applied to the measurement of basal levels of prolactin in the blood of 4 anoestrous lactating female, 4 anoestrous virgin female and 4 castrated male goats. For several hours after milking there was a chronic gradually decreasing release of prolactin in the lactating goats, such that the circulating levels of prolactin were, for 14–16 h of the day, at a much higher level than those in the virgin female or castrated male goats. The basal levels of prolactin in the castrated male goats (87–200ng/ml) were somewhat higher than those in the virgin females (47–77ng/ml). The effects of feeding and stress on prolactin levels are discussed.

Type
Research Article
Information
Journal of Dairy Research , Volume 40 , Issue 2 , June 1973 , pp. 235 - 245
Copyright
Copyright © Proprietors of Journal of Dairy Research 1973

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Ajika, K., Kalra, S. P., Fawcett, C. P., Krulich, L. & McCann, S. M. (1972). Endocrinology 90, 707.Google Scholar
Bryant, G. D. & Greenwood, F. C. (1968). Biochemical Journal 109, 831.Google Scholar
Bryant, G. D., Greenwood, F. C. & Linzell, J. L. (1968). Journal of Endocrinology 40, iv.Google Scholar
Bryant, G. D., Linzell, J. L. & Greenwood, F. C. (1970). Hormones 1, 26.Google Scholar
Clark, R. H. & Baker, B. L. (1964). Science 143, 375.CrossRefGoogle Scholar
Cowie, A. T. (1971). In Lactation: Proceedings of the 17th Easter School in Agricultural Science, University of Nottingham, 1970, p. 123. (Ed. Falconer, I. R..) London: Butterworth.Google Scholar
den Hollander, F. C. & Schuurs, A. H. W. M. (1971). In Radioimmunoassay Methods: European Workshop, 1970, p. 419. (Eds Kirkham, K. E. and Hunter, W. M..) Edinburgh and London: Churchill Livingstone.Google Scholar
Folley, S. J. & Knaggs, G. S. (1965). Journal of Endocrinology 33, 301.Google Scholar
Grosvenor, C. E. (1965). Endocrinology 76, 340.CrossRefGoogle Scholar
Grosvenor, C. E., McCann, S. M. & Nallar, R. (1965). Endocrinology 76, 883.Google Scholar
Hart, I. C. (1972 a). Journal of Endocrinology 55, 51.Google Scholar
Hart, I. C. (1972 b). Journal of Endocrinology 55, xxviii.Google Scholar
Johke, T. (1969). Endocrinologia Japonica 16, 179.Google Scholar
Johke, T. (1970). Endocrinologia Japonica 17, 393.Google Scholar
McAtee, J. W. & Trenkle, A. (1971). Endocrinology 89, 730.CrossRefGoogle Scholar
Morgan, C. R., Sorenson, R. L. & Lazarow, A. (1964 a). Diabetes 13, 1.CrossRefGoogle Scholar
Morgan, C. R., Sorenson, R. L. & Lazarow, A. (1964 b). Diabetes 13, 579.Google Scholar
Neill, J. D. (1970). Endocrinology 87, 1192.Google Scholar
Nicoll, C. S., Talwalker, P. K. & Meites, J. (1960). American Journal of Physiology 198, 1103.Google Scholar
Schams, D. & Karg, H. (1970). Zentralblatt für Veterinärmedizin A 17, 193.CrossRefGoogle Scholar
Wide, L. (1969). In Immunoassay of Gonadotrophins. 1st Karolinska Symposium on Research Methods in Reproductive Endocrinology, 1969, p. 207.Google Scholar
(Ed. Diczfalusy, E..) Acta Endocrinologica, Supplement 142.Google Scholar