Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-26T18:12:34.262Z Has data issue: false hasContentIssue false
  • English
  • Français

Associations of β-hydroxybutyrate, cholesterol, triglycerides and high-density lipoproteins to non-esterified fatty acids pre- and postpartum

Published online by Cambridge University Press:  15 November 2016

Julia Ruoff ,
Sandra Bertulat ,
Onno Burfeind  and
Wolfgang Heuwieser
Julia Ruoff
Affiliation:
Clinic for Animal Reproduction, Faculty of Veterinary Medicine, Freie Universität Berlin, Königsweg 65, 14163 Berlin, Germany
Sandra Bertulat
Affiliation:
Clinic for Animal Reproduction, Faculty of Veterinary Medicine, Freie Universität Berlin, Königsweg 65, 14163 Berlin, Germany
Onno Burfeind
Affiliation:
Clinic for Animal Reproduction, Faculty of Veterinary Medicine, Freie Universität Berlin, Königsweg 65, 14163 Berlin, Germany
Wolfgang Heuwieser*
Affiliation:
Clinic for Animal Reproduction, Faculty of Veterinary Medicine, Freie Universität Berlin, Königsweg 65, 14163 Berlin, Germany
*
*For correspondence; e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

While laboratory tests for measuring the concentration of NEFA in serum are well established, a point of care test to determine NEFA on farm is not available. Several hand-held measuring devices, however, have been validated for measuring β-hydroxybutyrate (BHBA) in cattle or cholesterol, triglycerides (TAG), and high-density lipoproteins (HDL) in human medicine, respectively. The objective of this study was to evaluate the association between NEFA and different parameters related to lipid metabolism. Specifically, we set out to determine if it is feasible to predict the concentration of NEFA by means of surrogate measures. The concentration of BHBA was determined by a hand-held device evaluated for use in cows, whereas the concentrations of the other parameters were determined by laboratory analysis because hand-held devices for cholesterol, TAG and HDL are only evaluated for human medicine so far. A total of 254 cows were included in the trial. One blood sample was taken from each cow between d 10 and d 1 prepartum. Second and third samples were collected on d 2 and d 10 postpartum, respectively. The coefficients of correlation between parameters were calculated and a receiver-operator characteristic curve analysis has been used. The prediction of NEFA concentrations using only one of the parameters was insufficient. However, a NEFA concentration ≥0·5 mEq/l could be predicted with a high sensitivity (i.e. Se = 0·88) and specificity (i.e. Sp = 0·93) from d 3 to d 1 prepartum and a NEFA concentration ≥0·7 mEq/l could be reliably predicted on d 2 postpartum (i.e. AUC = 0·89, Se = 0·89, Sp = 0·76) when using a combination of BHBA, cholesterol and TAG as surrogates. Overall, our results suggest that a combination of different parameters of lipid metabolism could be used as surrogates for NEFA.

Keywords

nonesterified fatty acidsß-hydroxybutyratelipid metabolismhand-held devicedairy cow
Type
Research Article
Information
Journal of Dairy Research , Volume 83 , Issue 4 , November 2016 , pp. 447 - 452
Copyright
Copyright © Proprietors of Journal of Dairy Research 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bach, KD, Heuwieser, W & McArt, JA 2016 Technical note: Comparison of 4 electronic handheld meters for diagnosing hyperketonemia in dairy cows. Journal of Dairy Science 96 198–209 Google Scholar
Bobe, G, Young, JW & Beitz, DC 2004 Invited review: pathology, etiology, prevention, and treatment of fatty liver in dairy cows. Journal of Dairy Science 87 3105–3124 CrossRefGoogle ScholarPubMed
Chapinal, N, Carson, M, Duffield, TF, Capel, M, Godden, S, Overton, M, Santos, JE & LeBlanc, SJ 2011 The association of serum metabolites with clinical disease during the transition period. Journal of Dairy Science 94 4897–4903 CrossRefGoogle ScholarPubMed
Coqueiro, RD, Santos, MC, Neto, JD, Queiroz, BM, Brugger, NA & Barbosa, AR 2013 Validity of a portable glucose, total cholesterol, and triglycerides multi-analyzer in adults. Biological Research for Nursing 16 288–294 Google Scholar
Ferreira, CE, Franca, CN, Correr, CJ, Zucker, ML, Andriolo, A & Scartezini, M 2015 Clinical correlation between a point-of-care testing system and laboratory automation for lipid profile. Clinica Chimica Acta; International Journal of Clinical Chemistry 446 263–266 CrossRefGoogle ScholarPubMed
Hammon, HM, Sturmer, G, Schneider, F, Tuchscherer, A, Blum, H, Engelhard, T, Genzel, A, Staufenbiel, R & Kanitz, W 2009 Performance and metabolic and endocrine changes with emphasis on glucose metabolism in high-yielding dairy cows with high and low fat content in liver after calving. Journal of Dairy Science 92 1554–1566 Google Scholar
Herdt, TH 2000 Ruminant adaptation to negative energy balance. Influences on the etiology of ketosis and fatty liver. The Veterinary Clinics of North America. Food Animal Practice 16 215–230 CrossRefGoogle Scholar
Iwersen, M, Falkenberg, U, Voigtsberger, R, Forderung, D & Heuwieser, W 2009 Evaluation of an electronic cowside test to detect subclinical ketosis in dairy cows. Journal of Dairy Science 92 2618–2624 Google Scholar
Iwersen, M, Klein-Jobstl, D, Pichler, M, Roland, L, Fidlschuster, B, Schwendenwein, I & Drillich, M 2013 Comparison of 2 electronic cowside tests to detect subclinical ketosis in dairy cows and the influence of the temperature and type of blood sample on the test results. Journal of Dairy Science 96 7719–7730 CrossRefGoogle ScholarPubMed
Janovick, NA, Boisclair, YR & Drackley, JK 2011 Prepartum dietary energy intake affects metabolism and health during the periparturient period in primiparous and multiparous Holstein cows. Journal of Dairy Science 94 1385–1400 Google Scholar
Kluger, EK, Dhand, NK, Baral, RM, Snow, DH, Malik, R & Govendir, M 2010 Assessment of the accutrend GCT and PTS CardioChek meters to measure blood triglyceride concentrations in cats. Journal of Feline Medicine and Surgery 12 458–465 CrossRefGoogle ScholarPubMed
Kurpinska, AK, Jarosz, A, Ozgo, M & Skrzypczak, WF 2015 Changes in lipid metabolism during last month of pregnancy and first two months of lactation in primiparous cows – analysis of apolipoprotein expression pattern and changes in concentration of total cholesterol, HDL, LDL, triglycerides. Polish Journal of Veterinary Sciences 18 291–298 Google Scholar
Mahrt, A, Burfeind, O, Voigtsberger, R, Muller, A & Heuwieser, W 2014 Evaluation of a new electronic handheld meter for measurement of beta-hydroxybutyric acid in dairy cows. Tierarztliche Praxis. Ausgabe G, Grosstiere/Nutztiere 42 5–10 Google Scholar
McArt, JAA, Nydam, DV, Oetzel, GR, Overton, TR & Ospina, PA 2013 Elevated non-esterified fatty acids and β-hydroxybutyrate and their association with transition dairy cow performance. Veterinary Journal 198 560–570 Google Scholar
McCarthy, MM, Mann, S, Nydam, DV, Overton, TR & McArt, JA 2015 Short communication: concentrations of nonesterified fatty acids and beta-hydroxybutyrate in dairy cows are not well correlated during the transition period. Journal of Dairy Science 98 6284–6290 Google Scholar
Pepe, MS, Cai, T & Longton, G 2006 Combining predictors for classification using the area under the receiver operating characteristic curve. Biometrics 62 221–229 Google Scholar
Quiroz-Rocha, GF, LeBlanc, S, Duffield, T, Wood, D, Leslie, KE & Jacobs, RM 2009 Evaluation of prepartum serum cholesterol and fatty acids concentrations as predictors of postpartum retention of the placenta in dairy cows. Journal of the American Veterinary Medical Association 234 790–793 CrossRefGoogle ScholarPubMed
Reynolds, CK, Aikman, PC, Lupoli, B, Humphries, DJ & Beever, DE 2003 Splanchnic metabolism of dairy cows during the transition from late gestation through early lactation. Journal of Dairy Science 86 1201–1217 Google Scholar
Streyl, D, Sauter-Louis, C, Braunert, A, Lange, D, Weber, F & Zerbe, H 2011 Establishment of a standard operating procedure for predicting the time of calving in cattle. Journal of Veterinary Science 12 177–185 Google Scholar
Turk, R, Juretić, D, Gereš, D, Turk, N, Rekić, B, Simeon-Rudolf, V, Robić, M & Svetina, A 2005 Serum paraoxonase activity in dairy cows during pregnancy. Research in Veterinary Science 79 15–18 Google Scholar
van den Top, AM, Wensing, T, Geelen, MJ, Wentink, GH, van't Klooster, AT & Beynen, AC 1995 Time trends of plasma lipids and enzymes synthesizing hepatic triacylglycerol during postpartum development of fatty liver in dairy cows. Journal of Dairy Science 78 2208–2220 Google Scholar