Hostname: page-component-669899f699-qzcqf Total loading time: 0 Render date: 2025-04-26T01:07:45.105Z Has data issue: false hasContentIssue false
  • English
  • Français

Association of STAT1 gene with milk fat and protein yield in Holstein Friesian crossbred cattle maintained in the sub-tropical climate of India

Published online by Cambridge University Press:  23 October 2024

Anika Sharma ,
Simarjeet Kaur ,
Jaspreet Singh Arora  and
Neeraj Kashyap
Anika Sharma*
Affiliation:
Department of Animal Genetics and Breeding, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
Simarjeet Kaur
Affiliation:
Department of Animal Genetics and Breeding, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
Jaspreet Singh Arora
Affiliation:
Department of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
Neeraj Kashyap
Affiliation:
Department of Animal Genetics and Breeding, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
*
Corresponding author: Anika Sharma; Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Signal transducers and activators of transcription (STAT) genes are involved in signal mediation of various hormones and cytokines. STAT1 located on chromosome number 2 is involved in mammary gland development and is associated with milk composition traits in bovines. This study aimed to find any relationship and impact of STAT1/BspHI gene with milk fat and protein yields in a herd of Holstein Friesian (HF) crossbred cattle of sub-tropical climate of Northern India. Milk composition data of 535 adult HF crossbred cows for a period of 12 years was collected from the records maintained at Livestock Farm, Guru Angad Dev Veterinary and Animal Sciences University. First lactation data of 222 animals was chosen for further analysis. After data correction for non-genetic factors (season of calving, period of calving, interaction effect of season and period of calving and age at first calving) these animals were categorised into two groups based on corrected high and low milk fat and protein yields. Forty animals were then selected for blood collection and further laboratory analysis. Amplified using PCR-RFLP technique, the 314 bp STAT1 gene was digested using BspHI restriction enzyme. C-T polymorphism at nucleotide position 201 and 260 of the STAT1 amplicon was observed. At 201, for genotype AA and Aa, the genotypic frequencies were 0.80 and 0.20%. At 260, for genotype BB and Bb, the genotypic frequencies were 0.25 and 0.75%. Least square analysis showed a significant association of all genotypes with milk fat and protein yields. Hence, STAT1 can be used as a potential candidate gene to aid in better animal selection in breeding programmes.

Keywords

HF crossbred cattlemilk fat yieldmilk protein yieldPCR-RFLPSTAT1
Type
Research Article
Information
Journal of Dairy Research , Volume 91 , Issue 3 , August 2024 , pp. 262 - 266
Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

Ardicli, S, Soyudal, B, Samli, H, Dincel, D and Balci, F (2018) Effect of STAT1, OLR1, CSN1S1, CSN1S2, and DGAT1 genes on milk yield and composition traits of Holstein breed. Revista Brasileira de Zootecnia 47, 1–9.CrossRefGoogle Scholar
Ashwell, MS, Heyen, DW, Sonstegard, TS, Van Tassell, CP, Da, Y, Van-Raden, PM, Ron, M, Weller, JI and Lewin, HA (2004) Detection of quantitative trait loci affecting milk production, health, and reproductive traits in Holstein cattle. Journal of Dairy Science 87(2), 468–475.CrossRefGoogle ScholarPubMed
Askari, G, Ansari-Mahyari, S, Rahimi-Mianji, G and Asadollahpour-Nanaei, H (2013) Effect of STAT1 variants on milk production traits in Esfahan Holstein Cows. International Journal of Advanced Biological and Biomedical Research 1(8), 851–857.Google Scholar
Band, MR, Larson, JH, Rebeiz, M, Green, CA, Heyen, DW, Donovan, J, Windish, R, Steining, C, Mahyuddin, P, Womack, JE and Lewin, HA (2000) An ordered comparative map of the cattle and human genomes. Genome Research 10(9), 1359–1368.CrossRefGoogle ScholarPubMed
Basic Animal Husbandry Statistics (2023) Government of India, Ministry of Fisheries Animal Husbandry and Dairying, Department of Animal Husbandry & Dairying (DAHD), 29–49.Google Scholar
Boutinaud, M and Jammes, H (2004) Growth hormone increases Stat5 and Stat1 expression in lactating goat mammary gland: a specific effect compared to milking frequency. Domestic Animal Endocrinology 27, 363–378.CrossRefGoogle ScholarPubMed
Cobanoglu, O, Zaitoun, I, Chang, YM, Shook, GE and Khatib, H (2006) Effects of the signal transducer and activator of transcription 1 (STAT1) gene on milk production traits in Holstein dairy cattle. Journal of Dairy Science 89, 4433–4437.CrossRefGoogle ScholarPubMed
Cobanoglu, O, Gurcan, EK, Cankaya, S, Kul, E and Abaci, HS (2016) The detection of STAT1 gene influencing milk related traits in Turkish Holstein and Jersey cows. Journal of Agricultural Science and Technology A 6(4), 261–269Google Scholar
Darnell, JE (1997) STATs and gene regulation. Science (New York, N.Y.) 277(5332), 1630–1635.CrossRefGoogle ScholarPubMed
Deng, T, Pang, C, Zhu, P, Liao, B, Zhang, M, Yang, B and Liang, X (2015) Molecular cloning and expression analysis of the STAT1 gene in the water buffalo (Bubalus bubalis). Tropical Animal Health and Production 47(1), 53–59.CrossRefGoogle ScholarPubMed
Grechko, VV (2002). Molecular DNA markers in phylogeny and systematics. Russian Journal of Genetics 38 (8): 851–868.CrossRefGoogle ScholarPubMed
Hasan, F, Khaerunnisa, I and Daulay, AH (2021). Signal transducer and activator of transcription 1 gene polymorphism in Indonesian river buffalo. IOP Conference Series: Earth and Environmental Science, 782(2).Google Scholar
Khandare, PM, Kale, DS, Koringa, PG, Patil, DV and Dhok, AP (2020) DNA sequence variation within STAT1 gene of Gaolao cattle. 9, 2883–2893.CrossRefGoogle Scholar
Khatib, H, Heifetz, E and Dekkers, JC (2005) Association of the protease inhibitor gene with production traits in Holstein dairy cattle. Journal of Dairy Science 88, 1208–1213.CrossRefGoogle ScholarPubMed
Khatib, H, Leonard, S, Schutzkus, V, Luo, W and Chang, YM (2006) Association of the OLR1 gene with milk composition in Holstein dairy cattle. Journal of Dairy Science 89, 1753–1760.CrossRefGoogle ScholarPubMed
Khatib, H, Huang, W, Mikheil, D, Schutzkus, V and Monson, RL (2009) Effects of signal transducer and activator of transcription (STAT) genes STAT1 and STAT3 genotypic combinations on fertilization and embryonic survival rates in Holstein cattle. Journal of Dairy Science 92, 6186–6191.CrossRefGoogle ScholarPubMed
Khatkar, MS, Thomson, PC, Tammen, I and Raadsma, HW (2004) Quantitative trait loci mapping in dairy cattle: review and meta-analysis. Genetics, Selection and Evolution 36, 163–190.CrossRefGoogle ScholarPubMed
Kumar, M, Vohra, V, Ratwan, P and Chakravarty, AK (2015) Exploring polymorphism in 3′UTR region of STAT1 gene in different Buffalo breeds. Indian Journal of Dairy Science 68, 473–476.Google Scholar
Kwon, JM and Goate, AM (2000) The candidate gene approach. Alcohol Research and Health 24, 164–168.Google ScholarPubMed
Meyer, T, Marg, A, Lemke, P, Wiesner, B and Vinkemeier, U (2003) DNA binding controls inactivation and nuclear accumulation of the transcription factor Stat1. Genes and Development 17, 1992–2005.CrossRefGoogle ScholarPubMed
Ron, M, Feldmesser, E, Golik, M, Tager, I, Kliger, D, Reiss, V, Domochovsky, R, Alus, O, Seroussi, E, Ezra, E and Weller, JI (2004) A complete genome scan of the Israeli Holstein population for quantitative trait loci by a daughter design. Journal of Dairy Science 87, 476–490.CrossRefGoogle ScholarPubMed
Rychtářová, J, Sztankóová, Z, Kyselová, J, Zink, V, Štípková, M, Vacek, M and Štolc, L (2014) Effect of DGAT1, BTN1A1, OLR1, and STAT1 genes on milk production and reproduction traits in the Czech fleck vieh breed. Czech Journal of Animal Science 59(2), 45–53.CrossRefGoogle Scholar
Teneva, A and Petrovic, MP (2010) Application of molecular markers in livestock improvement. Biotechnology in Animal Husbandry 26, 135–154.CrossRefGoogle Scholar
Vafaee, H and Mashhadi, H (2016) Association of single nucleotide polymorphism of STAT1 gene with milk production traits in Brown Swiss cattle. Animal Production 18, 377–386.Google Scholar
Wakchaure, R, Ganguly, S, Para, PA, Praveen, PK, Kumar, A and Sharma, S (2015) Development of crossbred cattle in India: a review. International Journal of Emerging Technology and Advanced Engineering 5, 75–77.Google Scholar
Supplementary material: File

Sharma et al. supplementary material

Sharma et al. supplementary material
Download Sharma et al. supplementary material(File)
File 120.5 KB