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The association between bedding material and the bacterial counts of Staphylococcus aureus, Streptococcus uberis and coliform bacteria on teat skin and in teat canals in lactating dairy cattle

Published online by Cambridge University Press:  28 February 2013

Jan-Hendrik Paduch ,
Elmar Mohr  and
Volker Krömker
Jan-Hendrik Paduch
Affiliation:
Faculty II, Department of Microbiology, University of Applied Sciences and Arts Hannover, Heisterbergallee 12, 30453 Hannover, Germany Faculty of Agricultural and Environmental Sciences, University of Rostock, Chair of Animal Health and Animal Welfare, Justus-von-Liebig-Weg 8, 18059 Rostock, Germany
Elmar Mohr
Affiliation:
Faculty of Agricultural and Environmental Sciences, University of Rostock, Chair of Animal Health and Animal Welfare, Justus-von-Liebig-Weg 8, 18059 Rostock, Germany
Volker Krömker*
Affiliation:
Faculty II, Department of Microbiology, University of Applied Sciences and Arts Hannover, Heisterbergallee 12, 30453 Hannover, Germany
*
*For correspondence; e-mail: [email protected]
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Abstract

Several mastitis-causing pathogens are able to colonize the bovine teat canal. The objective of this study was to investigate the association between the treatment of sawdust bedding with a commercial alkaline conditioner and the bacterial counts on teat skin and in the teat canal. The study used a crossover design. Ten lactating Holstein cows that were free of udder infections and mastitis were included in the study. The animals were bedded on either untreated sawdust or sawdust that had been treated with a hydrated lime-based conditioner. Once a day, fresh bedding material was added. After 3 weeks, the bedding material was removed from the cubicles, fresh bedding material was provided, and the cows were rotated between the two bedding material groups. Teat skin and teat canals were sampled using the wet and dry swab technique after weeks 1, 2, 3, 4, 5 and 6. Staphylococcus aureus, Streptococcus uberis, Escherichia coli and other coliform bacteria were detected in the resulting agar plate cultures. The treatment of the bedding material was associated with the teat skin bacterial counts of Str. uberis, Esch. coli and other coliform bacteria. An association was also found between the bedding material and the teat canal bacterial counts of coliform bacteria other than Esch. coli. For Staph. aureus, no associations with the bedding material were found. In general, the addition of a hydrated lime-based conditioner to sawdust reduces the population sizes of environmental pathogens on teat skin and in teat canals.

Keywords

Mastitisbedding materialmastitis causing pathogensteat flora
Type
Research Article
Information
Journal of Dairy Research , Volume 80 , Issue 2 , May 2013 , pp. 159 - 164
Copyright
Copyright © Proprietors of Journal of Dairy Research 2013

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References

Bey, RF, Reneau, JK & Farnsworth, RJ 2002 The role of bedding management in udder health. In Proceedings National Mastitis Council Annual Meeting, Orlando, Florida, USA, pp. 45–55Google Scholar
Bramley, AJ 1984 Streptococcus uberis udder infection – a major barrier to reducing mastitis incidence. British Veterinary Journal 140 328–335CrossRefGoogle Scholar
Bramley, AJ & Neave, FK 1975 Studies on the control of coliform mastitis in dairy cows. British Veterinary Journal 131 160–169CrossRefGoogle ScholarPubMed
Bramley, AJ, King, JS, Higgs, TM & Neave, FK 1979 Colonization of the bovine teat duct following inoculation with Staphylococcus aureus and Escherichia coli. British Veterinary Journal 135 149–162Google Scholar
Capuco, AV, Mein, GA, Nickerson, SC, Jack, LJW, Wood, DL, Bright, SA, Aschenbrenner, RA, Miller, RH & Bitman, J 1994 Influence of pulsationless milking on teat canal keratin and mastitis. Journal of Dairy Science 77 64–74CrossRefGoogle ScholarPubMed
Du Preez, JH 1985 Teat canal infections. Kieler Milchwirtschaftliche Forschungsberichte 37 267–273Google Scholar
Fairchild, TP, McArthur, BJ, Moore, JH & Hylton, WE 1982 Coliform counts in various bedding materials. Journal of Dairy Science 65 1029–1035CrossRefGoogle Scholar
German Veterinary Medical Society 2002 Leitlinien zur Bekämpfung der Mastitis des Rindes als Bestandsproblem [Guidelines for bovine mastitis control in dairy herds]. 4th edition. Gießen, Germany: German Veterinary Medical SocietyGoogle Scholar
Grunert, E 1990 Weiblicher Geschlechtsapparat und Euter [Female genital tract and udder]. pp. 472–548 (Eds Rosenberger, G, Dirksen, G, Gründer, HD & Stöber, M). Berlin, Hamburg, Germany: Die klinische Untersuchung des Rindes, Verlag Paul PareyGoogle Scholar
Haveri, M, Hovinen, M, Roslöf, A & Pyörälä, S 2008 Molecular types and genetic profiles of Staphylococcus aureus strains isolated from bovine intramammary infections and extramammary sites. Journal of Clinical Microbiology 46 3728–3735CrossRefGoogle ScholarPubMed
Hogan, JS & Smith, KL 1997 Bacteria counts in sawdust bedding. Journal of Dairy Science 80 1600–1605CrossRefGoogle ScholarPubMed
Hogan, JS, Smith, KL, Hoblet, KH, Todhunter, DA, Schoenberger, PS, Hueston, WD, Pritchard, DE, Bowman, GL, Heider, LE, Brockett, BL & Conrad, HR 1989 Bacterial counts in bedding materials used on nine commercial dairies. Journal of Dairy Science 72 250–258Google Scholar
Hogan, JS, Bogacz, VL, Thompson, LM, Romig, S, Schoenberger, PS, Weiss, WP & Smith, KL 1999 Bacterial counts associated with sawdust and recycled manure bedding treated with commercial conditioners. Journal of Dairy Science 82 1690–1695CrossRefGoogle ScholarPubMed
Hughes, J 1999 Bedding systems and mastitis. In Proceedings British Mastitis Conference, Axient/Institute for Animal Health/Milk Development Council/Novartis Animal Health, Stoneleigh, pp. 73–78Google Scholar
Jain, NC 1979 Common mammary pathogens and factors in infection and mastitis. Journal of Dairy Science 62 128–134CrossRefGoogle ScholarPubMed
Kagkli, DM, Vancanneyt, M, Vandamme, P, Hill, C & Cogan, TM 2007 Contamination of milk by enterococci and coliforms from bovine faeces. Journal of Applied Microbiology 103 1393–1405CrossRefGoogle ScholarPubMed
Mein, GA, Neijenhuis, F, Morgan, WF, Reinemann, DJ, Hillerton, JE, Baines, JR, Ohnstad, I, Rasmussen, MD, Timms, L, Britt, JS, Farnsworth, R, Cook, N & Hemling, T 2001 Evaluation of bovine teat condition in commercial dairy herds: 1. Non-infectious factors. In Proceedings 2nd International Symposium on Mastitis and Milk Quality, Vancouver, Canada, pp. 347–351Google Scholar
Munoz, MA, Welcome, FL, Schukken, YH & Zadoks, RN 2007 Molecular epidemiology of two Klebsiella pneumoniae mastitis outbreaks on a dairy farm in New York State. Journal of Clinical Microbiology 45 3964–3971Google Scholar
Nickerson, SC 1987 Resistance mechanisms of the bovine udder: new implications for mastitis control at the teat end. Journal of the American Veterinary Medical Association 191 1484–1488Google ScholarPubMed
Paduch, JH & Krömker, V 2011 Colonization of the teat skin and the teat canal by mastitis pathogens in dairy cattle. Tierärztliche Praxis 39(G) 71–76Google Scholar
Paduch, JH, Mohr, E & Krömker, V 2012 The association between teat end hyperkeratosis and teat canal microbial load in lactating dairy cattle. Veterinary Microbiology 158 353–359Google Scholar
Paulrud, CO 2005 Basic concepts of the bovine teat canal. Veterinary Research Communincations 29 215–245Google Scholar
Piccinini, R, Cesaris, L, Daprà, V, Borromeo, V, Picozzi, C, Sechhi, C & Zecconi, A 2009 The role of teat skin contamination in the epidemiology of Staphylococcus aureus intramammary infections. Journal of Dairy Research 76 36–41CrossRefGoogle ScholarPubMed
Pryor, SM 2008 Bovine mastitis and ecology of Streptococcus uberis. Dissertation, University of Waikato, New ZealandGoogle Scholar
Quirk, T, Fox, LK, Hancock, DD, Capper, J, Wenz, J & Park, J 2012 Intramammary infections and teat canal colonization with coagulase-negative staphylococci after postmilking teat disinfection: species-specific responses. Journal of Dairy Science 95 1906–1912Google Scholar
Rendos, JJ, Eberhart, RJ & Kesler, EM 1975 Microbial populations of teat ends of dairy cows, and bedding materials. Journal of Dairy Science 58 1492–1500CrossRefGoogle Scholar
Smith, KL, Todhunter, DA & Schoenberger, PS 1985 Environmental mastitis: cause, prevalence, prevention. Journal of Dairy Science 68 1531–1553Google Scholar
Watts, JL, Salmon, SA & Yancey, RJ 1993 Use of modified Rambach agar to differentiate Streptococcus uberis from other mastitis streptococci. Journal of Dairy Science 76 1740–1743Google Scholar
Wellek, S & Blettner, M 2012 On the proper use of the crossover design in clinical trials: part 18 of a series on evaluation of scientific publications. Deutsches Ärzteblatt International 109 276–281Google Scholar
Williams, DM & Mein, GA 1985 The role of machine milking in the invasion of mastitis organisms and implications for maintaining low infection rates. Kieler Milchwirtschaftliche. Forschungsberichte 37 415–425Google Scholar
Zadoks, RN, Allore, HG, Barkema, HW, Sampimon, OC, Gröhn, YT & Schukken, YH 2001 Analysis of an outbreak of Streptococcus uberis mastitis. Journal of Dairy Science 84 590–599Google Scholar
Zdanowicz, M, Shelford, JA, Tucker, CB, Weary, DM & von Keyserlingk, MAG 2004 Bacterial populations on teat ends of dairy cows housed in free stalls and bedded with either sand or sawdust. Journal of Dairy Science 87 1694–1701CrossRefGoogle ScholarPubMed
Zecconi, A, Hamann, J, Bronzo, V & Ruffo, G 1992 Machine-induced teat tissue reactions and infection risk in a dairy herd free from contagious mastitis pathogens. Journal of Dairy Research 59 265–271Google Scholar