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Antimicrobial resistance profile of non-aureus Staphylococci isolates from buffalo, goat and sheep mastitis in the Northeast region of Brazil

Published online by Cambridge University Press:  07 September 2020

André de Souza Santos ,
Débora Costa Viegas de Lima ,
Atzel Candido Acosta Abad ,
Pollyanne Raysa Fernandes de Oliveira ,
José Givanildo da Silva ,
Guilherme Santana de Moura ,
Amanda Thaís Ferreira Silva ,
Vinícius da Silva Amorim ,
Mateus Matiuzzi da Costa  and
Rinaldo Aparecido Mota
André de Souza Santos
Affiliation:
Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, Brazil
Débora Costa Viegas de Lima
Affiliation:
Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, Brazil
Atzel Candido Acosta Abad
Affiliation:
Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, Brazil
Pollyanne Raysa Fernandes de Oliveira
Affiliation:
Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, Brazil
José Givanildo da Silva
Affiliation:
Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, Brazil
Guilherme Santana de Moura
Affiliation:
Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, Brazil
Amanda Thaís Ferreira Silva*
Affiliation:
Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, Brazil
Vinícius da Silva Amorim
Affiliation:
Department of Veterinary Medicine, Federal University of Vale do São Francisco, Petrolina, Brazil
Mateus Matiuzzi da Costa
Affiliation:
Department of Veterinary Medicine, Federal University of Vale do São Francisco, Petrolina, Brazil
Rinaldo Aparecido Mota
Affiliation:
Department of Veterinary Medicine, Federal Rural University of Pernambuco, Recife, Brazil
*
Author for correspondence: Amanda Thaís Ferreira Silva, Email: [email protected]
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Abstract

The study described in this Research Communication investigated the genotypic and phenotypic profiles of resistance to beta-lactams and other antimicrobials in non-aureus Staphylococci (NAS) isolated from buffalo, goat and sheep mastitis in the Northeast region of Brazil. A total of 190 isolates were analyzed and 42.3, 43.9 and 23.6% of them were positive for blaZ gene in buffalo, goat and sheep, respectively. Regarding the animal groups, in goats, amoxicillin was the antimicrobial with highest resistance index (72.7%), followed by penicillin G in buffaloes (51.9%) and ampicillin in sheep (43.1%). With regard to multiple antimicrobial resistance, 30.8% of NAS isolates from buffalo milk samples, 25.8% from goats and 25.0% from sheep presented multidrug-resistance. In the minimum inhibitory concentration (MIC) technique, amoxicillin MIC50 and MIC90 were 64 and 128 μg/ml, respectively, among isolates of the three animal species. In conclusion, high rates of resistance to beta-lactams are presented among NAS isolated from mastitis cases in buffaloes, goats and sheep in Northeast region of Brazil. These results provide an alert to animal and human health researchers, suggesting that the frequency of NAS needs to be reduced because they carry resistance genes which might increase the existing levels of antimicrobial resistance.

Keywords

Beta-lactamaseblaZ geneintramammary infectionminimal inhibitory concentrationmultidrug resistance
Type
Research Article
Information
Journal of Dairy Research , Volume 87 , Issue 3 , August 2020 , pp. 290 - 294
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation.

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