Hostname: page-component-745bb68f8f-5r2nc Total loading time: 0 Render date: 2025-01-09T03:34:07.331Z Has data issue: false hasContentIssue false
  • English
  • Français

Activation of milk lipase by serum proteins: possible role in the occurrence of lipolysis in raw bovine milk

Published online by Cambridge University Press:  01 June 2009

Roger A. Clegg
Roger A. Clegg
Affiliation:
The Hannah Research Institute, Ayr, Scotland, K A6 5HL
Get access Rights & Permissions [Opens in a new window]

Summary

The increase in unesterified fatty acid content of unpasteurized bulk milk in storage at 4 °C in the presence of known effectors of bovine milk lipoprotein lipase originating from bovine serum was studied. Bovine serum and high density lipoprotein (HDL) caused an increase in developed unesterified fatty acid levels whilst lipoprotein-free serum, apo-HDL, all individual apo-HDL tested, and the unfractionated C-peptide fraction were without lipolytic effect. In the presence of HDL-lipids, 2 C-peptides stimulated considerable lipolysis, as did the combination of HDL-lipid with unfractionated C-peptides. These characteristics of unesterified fatty acid development could be duplicated in milk whose endogenous lipolytic activity had been destroyed by heat treatment (75 °C for 5 min) and then restored by addition of purified bovine milk lipoprotein lipase. Radioactively labelled glycerol trioleate in milk was not hydrolysed in the same way as milk fat on the addition of serum liproproteins.

Type
Original Articles
Information
Journal of Dairy Research , Volume 47 , Issue 1 , February 1980 , pp. 61 - 70
Copyright
Copyright © Proprietors of Journal of Dairy Research 1980

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Castberg, H. B., Egelrud, I., Solberg, P. & Olivecrona, T. (1975). Journal of Dairy Research 42, 255–266.CrossRefGoogle Scholar
Chandan, R. C. & Shahani, K. M. (1964). Journal of Dairy Science 47, 471–480.CrossRefGoogle Scholar
Clegg, R. A. (1978). Biochemical Society Transactions 6, 1207–1210.CrossRefGoogle Scholar
Deeth, H. C. & Fitz-Gerald, C. H. (1975). International Dairy Federation Bulletin (Doc. 86), 24–34.Google Scholar
Dole, V. P. (1956). Journal of Clinical Investigation 35, 150–154.CrossRefGoogle Scholar
Downey, W. K. & Murphy, R. F. (1975). International Dairy Federation Bulletin, (Doc. 86), 19–23.Google Scholar
Driessen, F. M. & Stadhouders, J. (1974). Netherlands Milk and Dairy Journal 28, 130–145.Google Scholar
Egelrud, T. & Olivecrona, T. (1972). Journal of Biological Chemistry 247, 6212–6217.CrossRefGoogle Scholar
Fainaru, M., Havel, R. J. & Imaizumi, K. (1977). Biochimica et Biophysica Acta 490, 144–155.CrossRefGoogle Scholar
Fielding, C. J. & Fielding, P. E. (1976). Journal of Lipid Research 17, 248–256.CrossRefGoogle Scholar
Havel, R. J., Fielding, C. J., Olivecrona, T., Shore, V. G., Fielding, P. E. & Egelrud, T. (1973). Biochemistry 12, 1828–1833.CrossRefGoogle Scholar
Herbert, P. N., Shulman, R. S., Levy, R. I. & Fredrickson, D. S. (1973). Journal of Biological Chemistry 248, 4941–4946.CrossRefGoogle Scholar
Herrinoton, B. L. (1954). Journal of Dairy Science 37, 775–789.CrossRefGoogle Scholar
Iverius, P-H. & Ostlund-Lindqvist, A-M. (1976). Journal of Biological Chemistry 251, 7791–7795.CrossRefGoogle Scholar
Jensen, R. G. & Pitas, R. E. (1976). Journal of Dairy Science 59, 1203–1214.CrossRefGoogle Scholar
Jonas, A. (1972). Journal of Biological Chemistry 247, 7767–7772.CrossRefGoogle Scholar
Kinnunen, P. K. J., Huttunen, J. K. & Ehnholm, C. (1976). Biochimica et Biophysica Acta 450, 342–351.CrossRefGoogle Scholar
Korn, E. D. (1962). Journal of Lipid Research 3, 246–250.CrossRefGoogle Scholar
Lim, C. T. & Scanu, A. M. (1976). Artery 2, 483–496.Google Scholar
Nilsson-Ehle, P. & Schotz, M. C. (1976). Journal of Lipid Research 17, 536–541.CrossRefGoogle Scholar
Ostlund-Lindqvist, A-M. & Iverius, P-H. (1975). Biochemical and Biophysical Research Communications 65, 1447–1455.CrossRefGoogle Scholar
Shahani, K. M., Harper, W. J., Jensen, R. G., Parry, R. M. & Zittle, C. A. (1973). Journal of Dairy Science 56, 531–543.CrossRefGoogle Scholar
Sjöström, G. (1974). International Dairy Federation Bulletin (Doc. 82), 5–6.Google Scholar
Wang, C. S. & Smith, R. L. (1975). Analytical Biochemistry 63, 414–417.CrossRefGoogle Scholar