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The action of rennin on casein: the effect of modifying functional groups on the casein

Published online by Cambridge University Press:  01 June 2009

R. D. Hill  and
Raione R. Laing
R. D. Hill
Affiliation:
Division of Dairy Research, C.S.I.R.O., Melbourne, Australia
Raione R. Laing
Affiliation:
Division of Dairy Research, C.S.I.R.O., Melbourne, Australia
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Summary

k-Casein and whole casein when photo-oxidized in the presence of methylene blue lose the ability to clot when treated with rennin. Two effects are involved—first the photo-oxidation alters the k-casein fraction so that the rennin is unable to split off the glycopeptide fragment, and secondly, in whole casein the photo-oxidation interferes with the aggregation in the presence of Ca++ that normally follows rennin action. As a result of amino acid analysis and specific treatments which affect other photo-oxidizable side chains, it is concluded that both of these effects are caused by the alteration of histidines.

Type
Original Articles
Information
Journal of Dairy Research , Volume 32 , Issue 2 , June 1965 , pp. 193 - 201
Copyright
Copyright © Proprietors of Journal of Dairy Research 1965

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