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Accelerated cheese ripening with heat treated cells of Lactobacillus helveticus and a commercial proteolytic enzyme

Published online by Cambridge University Press:  01 June 2009

Ylva Ardö  and
Hans-Erik Pettersson
Ylva Ardö
Affiliation:
Swedish Dairies' Association, Central Laboratory, Box 205, S-201 22 Malmö, Sweden
Hans-Erik Pettersson
Affiliation:
Swedish Dairies' Association, Central Laboratory, Box 205, S-201 22 Malmö, Sweden
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Summary

Synergic effects of proteolytic enzymes from two different microbial sources on the ripening of Swedish hard cheese were studied. When extracellular proteolytic enzymes from Bacillus subtilis (Neutrase) and/or heat treated cells of Lactobacillus helveticus (now L. delbrueckii subsp. helveticus) were added to the cheese milk, cheese ripening was accelerated; Neutrase effectively hydrolysed casein to give a softer body. Addition of heat treated lactobacilli did not accelerate hydrolysis of casein, but accelerated the breakdown of peptides which increased the amount of amino acid N in the cheese and also enhanced the intensity of cheese flavour. A bitter taste which developed in cheeses with added Neutrase could be eliminated by the simultaneous addition of heat treated lactobacilli.

Type
Original articles
Information
Journal of Dairy Research , Volume 55 , Issue 2 , May 1988 , pp. 239 - 245
Copyright
Copyright © Proprietors of Journal of Dairy Research 1988

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References

REFERENCES

Anon. 1983 Accelerated ripening of cheese and cheese products. IDF Bulletin Document 157 33–39Google Scholar
Ardö, Y. 1986 Separation of peptidases using FPLC. 22nd International Dairy Congress, The Hague Posters, p. 191Google Scholar
Bartels, H. J., Johnson, M. E. & Olson, N. F. 1985 Accelerated ripening of Gouda cheese. 1. Effect and evaluation of thermophilic lactobacilli and streptococci on proteolysis and flavor development. Journal of Dairy Science 68 (Suppl. 1) 69Google Scholar
Cheeseman, G. C. 1963 Action of rennet and other proteolytic enzymes on casein in casein-agar gels. Journal of Dairy Research 30 17–22CrossRefGoogle Scholar
El Soda, M., Desmazeaud, M. J., Aboudonia, S. & Badran, A. 1982 Acceleration of cheese ripening by the addition of extracts from Lactobacillus helveticus, Lactobacillus bulgaricus and Lactobacillus lactis to the cheese curd. Milchwissenschaft 37 325–327Google Scholar
International Dairy Federation 1980 Starters in the manufacture of cheese. IDF Bulletin Document 129Google Scholar
Law, B. A. & Wigmore, A. S. 1982 Accelerated cheese ripening with food grade proteinases. Journal of Dairy Research 49 137–146CrossRefGoogle Scholar
Law, B. A. & Wigomore, A. S. 1983 Accelerated ripening of Cheddar cheese with a commercial proteinase and intracellular enzymes from starter streptococci. Journal of Dairy Research 50 519–525CrossRefGoogle Scholar
Lewis, W. H. P. & Harris, H. 1967 Human red cell peptidases. Nature 215 351–355CrossRefGoogle ScholarPubMed
Mogensen, M. T. Sode 1947 Determination of the degree of proteolytic decomposition in cheese with special reference to the formol titration. Meddelande Nr 21 fran Statens Mejeriförsök no. 21Google Scholar
Novo Industri A/S 1978 Modified Anson-Hemoglobin-method for the determination of proteolytic activity. Analytical Method AF 4/5-GBGoogle Scholar
Pettersson, H.-E. & Sjöström, G. 1975 Accelerated cheese ripening: a method for increasing the number of lactic starter bacteria in cheese without detrimental effect to the cheese-making process, and its effect on the cheese ripening. Journal of Dairy Research 42 313–326CrossRefGoogle Scholar
Scott, R. 1981 Cheesemaking Practice, 1st edn.London: Applied Science PublishersGoogle Scholar
Sneath, P. H. A. (Ed.) 1986 Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2. Baltimore, MD: Williams & WilkinsGoogle Scholar