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Xanthine oxidase/dehydrogenase in mammary gland of mouse: relationship to mammogenesis and lactogenesis in vivo and in vitro

Published online by Cambridge University Press:  01 June 2009

Thomas J. Hayden ,
Denise Brennan ,
Katherine Quirke  and
Paddie Murphy
Thomas J. Hayden
Affiliation:
Department of Zoology, University College Dublin, Belfield, Dublin 4, Irish Republic
Denise Brennan
Affiliation:
Department of Zoology, University College Dublin, Belfield, Dublin 4, Irish Republic
Katherine Quirke
Affiliation:
Department of Zoology, University College Dublin, Belfield, Dublin 4, Irish Republic
Paddie Murphy
Affiliation:
Department of Zoology, University College Dublin, Belfield, Dublin 4, Irish Republic
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Summary

Xanthine oxidase/dehydrogenase (XO/XDH) increases at mid gestation in mammary gland but not in liver of the mouse and remains elevated until the pups are weaned at 20 d post partum. The increase in enzyme activity is due neither to alteration in activators or inhibitors nor to a production of a variant enzyme with altered catalytic properties. The increase is preceded in vivo by a surge of prolactin-like activity (placental lactogen) in plasma, and prolactin is required for induction of XO/XDH in explant culture in vitro. Induction of XO/XDH in vivo and in vitro precedes the full histological differentiation of the gland. In addition, induction of XO/XDH in vitro occurs more rapidly and at lower concentrations of prolactin than does histological differentiation. Thus although XO/XDH is present in milk, increased XO/XDH activity is an early event in mammogenesis in vivo and in vitro rather than a terminal component of differentiation.

Type
Original Articles
Information
Journal of Dairy Research , Volume 58 , Issue 4 , November 1991 , pp. 401 - 409
Copyright
Copyright © Proprietors of Journal of Dairy Research 1991

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