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Use of serum amyloid A and milk amyloid A in the diagnosis of subclinical mastitis in dairy cows

Published online by Cambridge University Press:  29 July 2009

Gabriele Gerardi ,
Daniele Bernardini ,
Carla Azzurra Elia ,
Vanni Ferrari ,
Luciano Iob  and
Severino Segato
Gabriele Gerardi*
Affiliation:
Department of Veterinary Clinical Science, University of Padua – 35020 Legnaro (PD), Italy
Daniele Bernardini
Affiliation:
Department of Veterinary Clinical Science, University of Padua – 35020 Legnaro (PD), Italy
Carla Azzurra Elia
Affiliation:
Department of Animal Science, University of Padua – 35020 Legnaro (PD), Italy
Vanni Ferrari
Affiliation:
Department of Veterinary Clinical Science, University of Padua – 35020 Legnaro (PD), Italy
Luciano Iob
Affiliation:
Istituto Zooprofilattico delle Venezie – 35020 Legnaro (PD), Italy
Severino Segato
Affiliation:
Department of Animal Science, University of Padua – 35020 Legnaro (PD), Italy
*
*For correspondence; e-mail: [email protected]
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Abstract

Mastitis is the most frequent and costly disease in dairy herds, as it negatively affects yield and milk quality. The presence of clinical mastitis is quite easy to asses, whereas the diagnosis of the subclinical form can be more difficult and requires laboratory assays. Somatic cell count (SCC) is widely used as a rapid and low-cost indicator of mastitis, even if is not useful in discriminating between the clinical and subclinical form. As amyloid A has been investigated as a marker of mastitis, the aim of this study was to assess the potential value of measuring amyloid A in serum and milk and the correlation with SCC in the diagnosis of subclinical mastitis. The reliability of two different ELISA kits for the measurement of amyloid A in milk was also tested. During a 1-month trial period, 21 cows were assigned to three experimental groups according to their health status: 6 cows with clinical mastitis (CM), 10 cows with subclinical mastitis (SM) and 5 healthy cows (HE). Amyloid A was measured both in serum (SAA) and in quarter milk samples (mAA) with a serum ELISA kit, and in quarter milk samples (MAA) with a milk ELISA kit. SCC, total microbial count (TMC) and bacterial examination of the milk were also carried out. After a log transformation, the data were submitted to ANOVA and linear regression. TMC was significantly higher in cows with clinical mastitis, while no differences were observed between the other two experimental groups. SCC and MAA levels were significantly different among the three groups. mAA concentrations were similar between cows with subclinical and clinical mastitis, and SAA was not affected by mastitis. A significant correlation between SCC and MAA or mAA was detected, while no correlation was recorded between SAA and mAA. A close relationship between MAA and mAA was noticeable even at low concentrations, suggesting MAA as a potential physiological marker of subclinical mastitis.

Keywords

Subclinical mastitisserum amyloid Amilk amyloid A
Type
Research Article
Information
Journal of Dairy Research , Volume 76 , Issue 4 , November 2009 , pp. 411 - 417
Copyright
Copyright © Proprietors of Journal of Dairy Research 2009

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References

Adkinson, RW, Ingawa, KH, Blouin, DC & Nickerson, SC 1993 Distribution of clinical mastitis among quarters of the bovine udder. Journal of Dairy Science 76 3453–3459CrossRefGoogle ScholarPubMed
Åkerstedt, M, Persson Waller, K & Sternesjö, Å 2007 Haptoglobin and serum amyloid A in relation to the somatic cell count in quarter, cow composite and bulk tank milk sample. Journal of Dairy Research 74 198–203CrossRefGoogle Scholar
Åkerstedt, M, Persson Waller, K, Bach Larsen, L, Forsbäck, L & Sternesjö, Å 2008 Relationship between haptoglobin and serum amyloid A in milk and milk quality. International Dairy Journal 18 669–674CrossRefGoogle Scholar
Alsemgeest, SP, Kalsbeek, HC, Wensing, T, Koeman, JP, van Ederen, AM & Gruys, E 1994 Concentrations of serum amyloid-A (SAA) and haptoglobin (HP) as parameters of inflammatory diseases in cattle. Veterinary Quarterly 16 21–23CrossRefGoogle ScholarPubMed
Barkema, HW, Schukken, YH, Lam, TJGM, Galligan, DT, Beiboer, ML & Brand, A 1997 Estimation of interdipendence among quarters of the bovine udder with subclinical mastitis and implications for analysis. Journal of Dairy Science 80 1592–1599CrossRefGoogle Scholar
Biggadike, HJ, Ohnstad, I, Laven, RA & Hillerton, JE 2002 Evaluation of measurements of the conductivity of quarter milk samples for the early diagnosis of mastitis. Veterinary Record 150 655–658CrossRefGoogle ScholarPubMed
Boosman, R, Niewold, TA, Mutsaers, CW & Gruys, E 1989 Serum amyloid A concentrations in cows given endotoxin as an acute-phase stimulant. American Journal of Veterinary Research 50 1690–1694Google ScholarPubMed
Bramley, AJ, Cullor, JS, Erskine, RJ, Fox, LK, Harmon, RJ, Hogan, JS, Nickerson, SC, Oliver, SP, Smith, KL & Sordillo, LM 1996 Current Concepts of Bovine Mastitis. 4th Edn. Madison WI, USA: National Mastitis Council Inc.Google Scholar
Brolund, L 1985 Cell counts in bovine milk. Causes of variation and applicability for diagnosis of subclinical mastitis. Acta Veterinaria Scandinavica 80 1–123Google ScholarPubMed
Eckersall, PD, Young, FJ, McComb, C, Hogarth, CJ, Safi, S, Weber, A, McDonald, T, Nolan, AM & Fitzpatrick, JL 2001 Acute phase proteins in serum and milk from dairy cows with clinical mastitis. Veterinary Record 148 35–41CrossRefGoogle ScholarPubMed
Grönlund, U, Hulten, C, Eckersall, PD, Hogarth, C & Persson Waller, K 2003 Haptoglobin and serum amyloid A in milk and serum during acute and chronic experimentally induced Staphylococcus aureus mastitis. Journal of Dairy Research 70 379–386CrossRefGoogle ScholarPubMed
Grönlund, U, Hallén Sandgren, C & Persson Waller, K 2005 Haptoglobin and serum amyloid A in milk from dairy cows with chronic sub-clinical mastitis. Veterinary Research 36 191–198CrossRefGoogle ScholarPubMed
Hamann, J 2002 Relationships between somatic cell count and milk composition. Bulletin of the International Dairy Federation 372 56–59Google Scholar
Harmon, RJ, Eberhart, RJ, Jasper, DE, Langlois, BE & Wilson, RA 1990 Microbiological Procedures for the Diagnosis of Bovine Udder Infection. Arlington VA, USA: National Mastitis Council Inc.Google Scholar
Hillerton, IE & Berry, EA 2003 The management and treatment of enviromental streptococcal mastitis. Veterinary linics of North America. Food Animal Practice 19 157–169CrossRefGoogle Scholar
Hirvonen, J, Eklund, K, Teppo, AM, Huszenicza, G, Kulcsar, M, Saloniemi, H & Pyörälä, S 1999 Acute phase response in dairy cows with experimentally induced Escherichia coli mastitis. Acta Veterinaria Scandinavica 40 35–46CrossRefGoogle ScholarPubMed
Hoe, EG & Ruegg, PL 2005 Relationship between antimicrobial susceptibility of clinical mastitis pathogens and treatment outcome in cows. Journal of American Veterinary Medical Association 227 1461–1468CrossRefGoogle ScholarPubMed
Hogan, JS, González, RN, Harmon, RJ, Nickerson, SC, Oliver, SP, Pankey, JW & Smith, KL 1999 Laboratory Handbook on Bovine Mastitis. Madison WI, USA: National Mastitis Council Inc.Google Scholar
Hogarth, CJ, Fitzpatrick, JL, Nolan, AM & Eckersall, PD 2002 The acute phase response in bovine mastitis. Proceedings of the XXII World Buiatrics Congress, 18–23 August 2002, Hannover, Germany, p. 121CrossRefGoogle Scholar
Jacobsen, S, Niewold, TA, Kornalijnslijper, E, Toussaint, MJM & Gruys, E 2005 Kinetics of local and systemic isoforms of serum amyloid A in bovine mastitic milk. Veterinary Immunology and Immunopathology 104 21–23CrossRefGoogle ScholarPubMed
Kawasaki, M, Kawamura, S, Tsukahara, M, Morita, S, Komiya, M & Natsuga, M 2008 Near-infrared spectroscopic sensing system for on-line lik quality assessment in a milking robot. Computers and Electronics in Agriculture 63 22–27CrossRefGoogle Scholar
Kossaibati, MA, Hovi, M & Esslemont, RJ 1998 Incidence of clinical mastitis in dairy herds in England. Veterinary Record 143 649–653CrossRefGoogle ScholarPubMed
Le Roux, Y, Laurent, F & Moussaoui, F 2003 Polymorphonuclear proteolytic activity and milk composition change. Veterinary Research 34 629–645CrossRefGoogle ScholarPubMed
Lindmark-Månsson, H, Bränning, C, Aldén, G & Paulsson, M 2006 Relationship between somatic cell count, individual leukocyte populations and milk components in bovine udder quarter milk. International Dairy Journal 16 717–727CrossRefGoogle Scholar
Malle, E, Steinmetz, A & Raynes, JG 1993 Serum amyloid A (SAA): an acute phase protein and apolipoprotein: a review. Atherosclerosis 102 131–146CrossRefGoogle Scholar
McDonald, TL, Weber, A & Smith, JW 1991 A monoclonal antibody sandwich immunoassay for serum amyloid A (SAA) protein. Journal of Immunological Methods 144 149–155CrossRefGoogle ScholarPubMed
McDonald, TL, Larson, MA, Mack, DR & Weber, A 2001 Elevated extra-hepatic expression and secretion of mammary-associated serum amyloid A 3 (M-SAA3) into colostrum. Veterinary Immunology and Immunopathology 83 203–211CrossRefGoogle Scholar
Molenaar, A, Rajan, G, Pearson, M, Miles, M, Petrova, R, Davis, S & Stelwagen, K 2002 A serum amyloid protein homologue is expressed by the mammary gland in a similar pattern to lactoferrin. Proceedings of the 3rd European Colloquium on Food Safety and Acute Phase Proteins, 23–25 May 2002, Doorn, The NetherlandsGoogle Scholar
Murata, H, Shimada, N & Yoshioka, M 2004 Current research on acute phase proteins in veterinary diagnosis: an overview. Veterinary Journal 168 28–40CrossRefGoogle ScholarPubMed
Nielsen, BH, Iacobsen, S, Andersen, PH, Niewold, TA & Heegard, PMH 2004 Acute phase protein concentrations in serum and milk from healthy cows, cows with clinical mastitis and cows with extramammary inflammatory conditions. Veterinary Record 154 361–365CrossRefGoogle ScholarPubMed
O'Mahony, MC, Healy, AM, Harte, D, Walshe, KG, Torgenson, PR & Doherty, ML 2006 Milk amyloid A: Correlation with cellular indices of mammary inflammation in cows with normal and raised serum amyloid A. Research in Veterinary Science 80 155–161CrossRefGoogle ScholarPubMed
Pedersen, LH, Aalbaeck, B, Røntved, CM, Ingvartsen, KL, Sorensen, NS, Heegard, PM & Jensen, HE 2003 Early pathogenesis and inflammatory response in experimental bovine mastitis due to Streptococcus uberis. Journal of Comparative Pathology 128 156–164CrossRefGoogle ScholarPubMed
Pepys, MB & Baltz, ML 1983 Acute phase proteins with special reference to C-reactive protein and related proteins (Pentaxins) and serum amyloid A protein. Advances in Immunology 34 141–212CrossRefGoogle ScholarPubMed
Pyörälä, S & Taponen, S 2009 Coagulase-negative staphylococci—Emerging mastitis pathogens. Veterinary Microbiology 134 3–8CrossRefGoogle ScholarPubMed
Radostits, OM, Gay, CC, Hinchcliff, KW & Constable, PD 2007 Veterinary Medicine. A Textbook of the Diseases of Cattle, Horses, Sheep, Pigs and Goats. 10th Edn. St Louis MO, USA: Saunders Elsevier p. 675Google Scholar
SAS/STAT 2002 User's Guide. Release, 9.1 Statistics. SAS Inst. Inc., Cary NC, USAGoogle Scholar
Schepers, AJ, Lam, TJ, Schukken, YH, Wilmink, JB & Hanekamp, WJ 1997 Estimation of variance components for somatic cell counts to determine thresholds for uninfected quarters. Journal of Dairy Science 80 1833–1840CrossRefGoogle ScholarPubMed
Sears, PM, Smith, BS, English, PB, Herer, PS & Gonzalez, RN 1990 Shedding pattern of Staphylococcus aureus from bovine intramammary infections. Journal of Dairy Science 73 2785–2795CrossRefGoogle ScholarPubMed
Tsenkova, R, Atanassova, S, Kawano, S & Toyoda, K 2001 Somatic cell count determination in cow's milk by near-infrared spectroscopy: a new diagnostic tool. Journal of Animal Science 79 2550–2557CrossRefGoogle ScholarPubMed
Uhlar, CM & Whitehead, AS 1999 Serum amyloid A, the major vertebrate acute-phase reactant. European Journal of Biochemistry 265 501–523CrossRefGoogle ScholarPubMed