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Use of liposomes for proteinase addition to Cheddar cheese

Published online by Cambridge University Press:  01 June 2009

Barry A. Law  and
Joseph S. King
Barry A. Law
Affiliation:
*National Institute for Research in Dairying (University of Reading), Shinfield, Reading RG2 9AT, UK
Joseph S. King
Affiliation:
*National Institute for Research in Dairying (University of Reading), Shinfield, Reading RG2 9AT, UK
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Summary

A commercial neutral proteinase was encapsulated in liposomes (multilamellar) and incorporated into cheese curd via the cheese milk. The milk proteins were protected from proteinase attack during curd manufacture. A significant proportion of the liposome-entrapped enzyme was retained in the curd and its subsequent release into the cheese matrix was inferred from an increased rate of β-casein breakdown.

Type
Original Articles
Information
Journal of Dairy Research , Volume 52 , Issue 1 , February 1985 , pp. 183 - 188
Copyright
Copyright © Proprietors of Journal of Dairy Research 1985

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References

REFERENCES

Anderson, M. & Andrews, A. T. 1977 Progressive changes in individual milk protein concentrations associated with high somatic cell counts. Journal of Dairy Research 44 223–235CrossRefGoogle ScholarPubMed
Braidman, I. P. & Gregoriadis, G. 1977 Rapid partial purification of placental glucocerebroside β-glucosidase and its entrapment in liposomes. Biochemical Journal 164 439–445CrossRefGoogle ScholarPubMed
Chapman, H. R. & Burnett, J. 1972 Seasonal changes in the physical properties of milk for cheesemaking. Dairy Industries 37 207–211Google Scholar
Cliffe, A. J. & Law, B. A. 1982 A new method for the detection of microbial proteolytic enzymes in milk. Journal of Dairy Research 49 209–219CrossRefGoogle Scholar
Gregoriadis, G. 1976 Entrapment of enzymes in liposomes. Methods in Enzymology 44 218–227CrossRefGoogle ScholarPubMed
Law, B. A. 1984 Cheeses. In Advances in the Microbiology and Biochemistry of Cheese and Fermented Milks (EdsDavies, F. L. and Law, B. A.). London: Applied Science Publishers.Google Scholar
Law, B. A., Sharpe, M. E., Chapman, H. R. & Reiter, B. 1973 Relationship of milk fat globule membrane material to flavor development in Cheddar cheese. Journal of Dairy Science 56 716–723CrossRefGoogle Scholar
Law, B. A. & Wigmore, A. S. 1982 a Accelerated cheese ripening with food grade proteinases. Journal of Dairy Research 49 137–146Google Scholar
Law, B. A. & Wigmore, A. S. 1982 b Microbial proteinases as agents for accelerated cheese ripening. Journal of the Society of Dairy Technology 35 75–76CrossRefGoogle Scholar
Law, B. A. & Wigmore, A. S. 1983 Accelerated ripening of Cheddar cheese with a commercial proteinase and intracellular enzymes from starter streptococci. Journal of Dairy Research 50 519–525CrossRefGoogle Scholar
Olson, F., Hunt, C. A., Szoka, F. C., Vail, W. J. & Papahadjopoulos, D. 1979 Preparation of liposomes of defined size distribution by extrusion through polycarbonate membranes. Biochimica el Biophysica Acta 557 9–23Google Scholar