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Use of cheese whey for biomass production and spray drying of probiotic lactobacilli

Published online by Cambridge University Press:  26 March 2014

Luisina Lavari ,
Roxana Páez ,
Alejandra Cuatrin ,
Jorge Reinheimer  and
Gabriel Vinderola
Luisina Lavari
Affiliation:
INTA EEA Rafaela, Ruta 34 km 227, Santa Fe, Argentina
Roxana Páez
Affiliation:
INTA EEA Rafaela, Ruta 34 km 227, Santa Fe, Argentina
Alejandra Cuatrin
Affiliation:
INTA EEA Rafaela, Ruta 34 km 227, Santa Fe, Argentina
Jorge Reinheimer
Affiliation:
Facultad de Ingeniería Química, Instituto de Lactología Industrial (INLAIN, UNL-CONICET), Universidad Nacional del Litoral, Santiago del Estero 2829, Santa Fe (3000), Argentina
Gabriel Vinderola*
Affiliation:
Facultad de Ingeniería Química, Instituto de Lactología Industrial (INLAIN, UNL-CONICET), Universidad Nacional del Litoral, Santiago del Estero 2829, Santa Fe (3000), Argentina
*
*For correspondence; e-mail: [email protected]
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Abstract

The double use of cheese whey (culture medium and thermoprotectant for spray drying of lactobacilli) was explored in this study for adding value to this wastewater. In-house formulated broth (similar to MRS) and dairy media (cheese and ricotta whey and whey permeate) were assessed for their capacity to produce biomass of Lactobacillus paracasei JP1, Lb. rhamnosus 64 and Lb. gasseri 37. Simultaneously, spray drying of cheese whey-starch solution (without lactobacilli cells) was optimised using surface response methodology. Cell suspensions of the lactobacilli, produced in in house-formulated broth, were spray-dried in cheese whey-starch solution and viability monitored throughout the storage of powders for 2 months. Lb. rhamnosus 64 was able to grow satisfactorily in at least two of the in-house formulated culture media and in the dairy media assessed. It also performed well in spray drying. The performance of the other strains was less satisfactory. The growth capacity, the resistance to spray drying in cheese whey-starch solution and the negligible lost in viability during the storage (2 months), makes Lb. rhamnosus 64 a promising candidate for further technological studies for developing a probiotic dehydrated culture for foods, utilising wastewaters of the dairy industry (as growth substrate and protectant) and spray drying (a low-cost widely-available technology).

Keywords

Cheese wheywhey permeatericotta wheybiomassprobioticsspray drying
Type
Research Article
Information
Journal of Dairy Research , Volume 81 , Issue 3 , August 2014 , pp. 267 - 274
Copyright
Copyright © Proprietors of Journal of Dairy Research 2014 

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References

Aguirre-Ezkauriatza, EJ, Aguilar Yáñez, JM, Ramírez Medrano, A & Alvarez, MM 2010 Production of probiotic biomass (Lactobacillus casei) in goat milk whey: comparison of batch, continuous and fed-batch cultures. Bioresource Technology 101 2837–2844CrossRefGoogle ScholarPubMed
Ananta, E, Volkert, M & Knorr, D 2005 Cellular injuries and storage stability of spray dried Lactobacillus rhamnosus GG. International Dairy Journal 15 399–409Google Scholar
A.O.A.C. 1995 Ash of milk – gravimetric method, in Official Methods of A.O.A.C. International, method 945.46 (33.2.10)Google Scholar
APHA 1998 Standard Methods for the Examination of the Water and Wastewater, 20th edition. APHA, AWWA, WEF, Washington, DCGoogle Scholar
Brusch Brinques, G, Do Carmo Peralba, M & Záchia Ayub, MA 2010 Optimization of probiotic and lactic acid production by Lactobacillus plantarum in submerged bioreactor systems. Journal of Industrial Microbiology and Biotechnology 37 205–212Google Scholar
Burns, P, Molinari, F, Vinderola, G & Reinheimer, J 2008 Suitability of whey and buttermilk for the production and frozen storage of cultures of probiotic lactobacilli. International Dairy Journal 61 156–164Google Scholar
Chávez, BE & Ledeboer, AM 2007 Drying of probiotics: optimization of formulation and process to enhance storage survival. Drying Technology 25 1193–1201Google Scholar
Corcoran, BM, Ross, RP, Fitzgerald, GF & Stanton, C 2004 Comparative survival of probiotic lactobacilli spray-dried in the presence of prebiotic substances. Journal of Applied Microbiology 96 1024–1039Google Scholar
Curda, L, Rudolfová, J, Stetina, J & Dryák, B 2006 Dried buttermilk containing galactooligosaccharides – process layout and its verification. Journal of Food Engineering 77 468–471Google Scholar
De Castro Cislaghi, FP, Dos Reis E Silva, C, Fritzen Freire, CB, Goulart Lorenz, J & Sant'Anna, E 2012 Bifidobacterium Bb-12 microencapsulated by spray drying with whey: survival under simulated gastrointestinal conditions, tolerance to NaCl, and viability during storage. Journal of Food Engineering 113 186–193Google Scholar
Desmond, C, Stanton, C, Fitzgerald, GF, Collins, K & Ross, RP 2002 Environmental adaptation of probiotic lactobacilli towards improvement of performance during spray drying. International Dairy Journal 12 183–190CrossRefGoogle Scholar
Dominguez, JM 2011 Drying. In Comprehensive Biotechnology, 2nd edition, Vol. 2, pp. 727–735 (Ed. Moo-Young, M). Elsevier, AmsterdamCrossRefGoogle Scholar
Fajardo Bernárdez, P, Rodríguez Amado, I, Pastrana Castro, L & Pérez Guerra, N 2008 Production of a potentially probiotic culture of Lactobacillus casei subsp. casei CECT 4043 in whey. International Dairy Journal 18 1057–1065Google Scholar
FAO/WHO 2002 Guidelines for the evaluation of probiotics in food. ftp://ftp.fao.org/es/esn/food/wgreport2.pdfGoogle Scholar
Fávaro Trindade, CS & Grosso, CRF 2002 Microencapsulation of L. acidophilus (La-05) and B. lactis (Bb-12) and evaluation of their survival at the pH values of the stomach and in bile. Journal of Microencapsulation 19 485–494Google Scholar
Fritzen Freire, CB, Prudêncio, ES, Ambonia, RDMC, Pinto, SS, Negrão Murakami, AN & Murakami, FS 2012 Microencapsulation of bifidobacteria by spray drying in the presence of prebiotics. Food Research International 45 306–312Google Scholar
Gardiner, GE, O'Sullivan, E, Kelly, J, Auty, MA, Fitzgerald, GF, Collins, JK, Ross, RP & Stanton, C 2000 Comparative survival rates of human-derived probiotic Lactobacillus paracasei and L. salivarius strains during heat treatment and spray drying. Applied and Environmental Microbiology 66 2605–2612Google Scholar
Gardiner, GE, Bouchier, P, O'Sullivan, E, Kelly, J, Collins, K, Fitzgerald, G, Ross, RP & Stanton, C 2002 A spray-dried culture for probiotic Cheddar cheese manufacture. International Dairy Journal 12 749–756CrossRefGoogle Scholar
Gharsallaoui, A, Roudaut, G, Chambin, O, Voilley, A & Saurel, R 2007 Applications of spray-drying in microencapsulation of food ingredients: an overview. Food Research International 40 1107–1121Google Scholar
Golowczyc, MA, Silva, J, Teixeira, P, De Antoni, G & Abraham, AG 2011 Cellular injuries of spray-dried Lactobacillus spp. isolated from kefir and their impact on probiotic properties. International Journal of Food Microbiology 144 556–560Google Scholar
Gregoret, V, Perezlindo, MJ, Vinderola, G, Reinheimer, J & Binetti, AA 2013 A comprehensive approach to determine the probiotic potential of human-derived Lactobacillus for industrial use. Food Microbiology 34 19–28Google Scholar
Guimarães, PM, Teixeira, JA & Domingues, L 2010 Fermentation of lactose to bioethanol by yeasts as part of integrated solutions for the valorization of cheese whey. Biotechnology Advances 28 375–384Google Scholar
IDF 1987a Determination of the Fat Content of Skim Milk, Whey and Buttermilk by the Rose-Gottlieb Gravimetric Method (reference method). Brussels: IDF (FIL-IDF Standard 22B)Google Scholar
IDF 1987b Milk, Cream and Evaporated milk – Dry Matter Oven Method (reference method). Brussels: IDF (FIL-IDF Standard 21B)Google Scholar
IDF 1993 Dried milk and dried cream. Determination of water content. Brussels: IDF (FIL-IDF Standard 26 A)Google Scholar
IDF 2001 Milk – Determination of Nitrogen Content – Part 1: Kjeldahl Method. Brussels: IDF (FIL-IDF Standard 20-2 ISO 8968-2)Google Scholar
Jantzen, M, Göpel, A & Beermann, C 2013 Direct spray drying and microencapsulation of probiotic Lactobacillus reuteri from slurry fermentation with whey. Journal of Applied Microbiology 115 1029–1036Google Scholar
Knorr, D 1998 Technological aspects related to microorganisms in functional foods. Trends in Food Science and Technology 9 295–306CrossRefGoogle Scholar
Lian, WC, Hsiao, HC & Chou, CC 2002 Survival of bifidobacteria after spray-drying. International Journal of Food Microbiology 74 79–86Google Scholar
Maragkoudakis, P, Nardi, T, Bovo, B, Corich, V & Giacomini, A 2010 Valorization of a milk industry by-product as substrate for microbial growth. Journal of Biotechnology 150S S1–S576Google Scholar
Muller, JA, Ross, RP, Fitzgerald, GF & Stanton, C 2009 Manufacture of probiotic bacteria. In Prebiotics and Probiotics Science and Technology, pp. 727–762 (Eds Charalampopoulos, D & Rastall, R). New York: Springer-VerlagGoogle Scholar
O'Riordan, K, Andrews, D, Buckle, K & Conway, P 2001 Evaluation of microencapsulation of a Bifidodobacterium strain with starch as an approach to prolonging viability during storage. Journal of Applied Microbiology 91 1059–1066Google Scholar
Páez, R, Lavari, L, Vinderola, G, Audero, G, Cuatrin, A, Zariztky, N & Reinheimer, J 2012 Effect of spray drying on lactobacilli viability and resistance to simulated gastrointestinal digestion. Food Research International 48 748–754Google Scholar
Páez, R, Lavari, L, Audero, G, Cuatrin, A, Zariztky, N, Reinheimer, J & Vinderola, G 2013 Study of the effects of spray drying on the functionality of probiotic lactobacilli. International Journal of Dairy Technology 66 155–161Google Scholar
Parente, E & Zottola, EA 1991 Growth of thermophilic starters in whey permeate media. Journal of Dairy Science 74 20–28Google Scholar
Peighambardoust, SH, Tafti, AG & Hesari, J 2011 Application of spray drying for preservation of lactic acid starter cultures: a review. Trends in Food Science and Technology 22 215–224Google Scholar
Pérez Guerra, N, Fajardo Bernárdez, P, Méndez, J, Cachaldor, P & Pastrana Castro, L 2007 Production of four potentially probiotic lactic acid bacteria and their evaluation as feed additives for weaned piglets. Animal Feed Science and Technology 134 89–107Google Scholar
Peters, RH 2005 Economic aspects of cheese making as influenced by whey processing options. International Dairy Journal 15 537–545Google Scholar
Prazeres, AR, Carvalho, F & Rivas, JJ 2012 Cheese whey management: a review. Journal of Environmental Management 110 48–68Google Scholar
Ricci, G, Borgo, F, Ferrario, C & Fortina, MG 2011 Cocoa powder as delivery medium for probiotic Lactobacillus strains. Advances in Microbiology 1 1–6CrossRefGoogle Scholar
Rodrigues, D, Sousa, S, Rocha Santos, T, Silva, JP, Sousa Lobo, JM, Costa, P, Amaral, MH, Pintado, MM, Gomes, AM, Malcata, FX & Freitas, AC 2011 Influence of L-cysteine, oxygen and relative humidity upon survival throughout storage of probiotic bacteria in whey protein-based microcapsules. International Dairy Journal 21 869–876CrossRefGoogle Scholar
Simpson, PJ, Stanton, C, Fitzgerald, GF & Ross, RP 2005 Intrinsic tolerance of Bifidobacterium species to heat and oxygen and survival following spray drying and storage. Journal of Applied Microbiology 99 493–501CrossRefGoogle ScholarPubMed
Siso, GMI 1996 The biotechnological utilization of cheese whey: a review. Bioresource Technology 57 1–11CrossRefGoogle Scholar
Soukoulis, C, Behboudi-Jobbehdar, S, Yonekura, L, Parmenter, C & Fisk, I 2013 Impact of milk protein type on the Viability and storage stability of microencapsulated Lactobacillus acidophilus NCIMB 701748 using spray drying. Food and Bioprocess Technology DOI: 10.1007/s11947-013-1120-XGoogle Scholar
Teixeira, PC, Castro, MH, Malcata, X & Kirby, RM 1995 Survival of Lactobacillus delbrueckii subsp. bulgaricus following spray drying. Journal of Dairy Science 78 1025–1031Google Scholar
Vinderola, G, Capellini, B, Villarreal, F, Suárez, V, Quiberoni, A & Reinheimer, J 2008 Usefulness of a set of simple in vitro tests for the screening and identification of probiotic candidate strains for dairy use. LWT- Food Science and Technology 41 1678–1688Google Scholar
Ying, DY, Sun, J, Sanguansri, L, Weerakkody, R & Augustin, MA 2012 Enhanced survival of spray-dried microencapsulated Lactobacillus rhamnosus GG in the presence of glucose. Journal of Food Engineering 109 597–602CrossRefGoogle Scholar
Zayed, G & Roos, YH 2004 Influence of trehalose and moisture content on survival of Lactobacillus salivarius subjected to freeze-drying and storage. Process Biochemistry 39 1081–1086Google Scholar