Hostname: page-component-669899f699-tzmfd Total loading time: 0 Render date: 2025-04-25T21:28:40.150Z Has data issue: false hasContentIssue false
  • English
  • Français

Untargeted lipidomics of ovine milk to analyse the influence of different diet regimens

Published online by Cambridge University Press:  10 August 2021

Cristina Manis ,
Margherita Addis ,
Maria Sitzia ,
Paola Scano ,
Viviana Garau ,
Andrea Cabiddu ,
Marco Caredda ,
Antonio Pirisi ,
Antonio Pulina  and
Pierpaolo Roggero
...Show all authors
Cristina Manis
Affiliation:
Dipartimento di Scienze della Vita e dell'Ambiente, Cittadella Universitaria di Monserrato, 09042, Monserrato, Italy
Margherita Addis
Affiliation:
Agris Sardegna, Servizio Ricerca Prodotti di Origine Animale, Loc. Bonassai, 07040Olmedo, Italy
Maria Sitzia
Affiliation:
Agris Sardegna, Servizio Ricerca Prodotti di Origine Animale, Loc. Bonassai, 07040Olmedo, Italy
Paola Scano
Affiliation:
Dipartimento di Scienze della Vita e dell'Ambiente, Cittadella Universitaria di Monserrato, 09042, Monserrato, Italy
Viviana Garau
Affiliation:
Dipartimento di Chimica e Farmacia, Scuola di specializzazione in Farmacia Ospedaliera, Università di Sassari, via Muroni 23a, 07100, Sassari, Italy
Andrea Cabiddu
Affiliation:
Agris Sardegna, Servizio Ricerca Prodotti di Origine Animale, Loc. Bonassai, 07040Olmedo, Italy
Marco Caredda
Affiliation:
Agris Sardegna, Servizio Ricerca Prodotti di Origine Animale, Loc. Bonassai, 07040Olmedo, Italy
Antonio Pirisi
Affiliation:
Agris Sardegna, Servizio Ricerca Prodotti di Origine Animale, Loc. Bonassai, 07040Olmedo, Italy
Antonio Pulina
Affiliation:
Dipartimento di Agraria and Nucleo di Ricerca sulla Desertificazione, Università di Sassari, viale Italia 39A, 07100Sassari, Italy
Pierpaolo Roggero
Affiliation:
Dipartimento di Agraria and Nucleo di Ricerca sulla Desertificazione, Università di Sassari, viale Italia 39A, 07100Sassari, Italy
Pierluigi Caboni*
Affiliation:
Dipartimento di Scienze della Vita e dell'Ambiente, Cittadella Universitaria di Monserrato, 09042, Monserrato, Italy
*
Author for correspondence: Pierluigi Caboni, Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

In this work we report a lipidomics approach to study the effects of two diet systems on the composition of ovine milk. Milk from two groups of Sarda sheep grazing on 40% (P40) and 60% (P60) of pasture were analyzed by a UHPLC-QTOF-MS analytical platform and data submitted to multivariate statistical analysis. Pairwise partial least square discriminant analysis of the lipid profile of the data was carried out to classify samples and to find discriminant lipids. The two dietary groups were characterized by differences in triacylglycerols, phosphocholines and phosphatidylethanolamines levels. Discriminants of the P40 group were TG and PC containing in their backbone saturated medium chain FA thus suggesting greater de novo fatty synthesis in the mammary gland. On the other hand, the P60 group was characterized by TG and PC formed by unsaturated long chain FA originating from the diet or from lipid mobilization.

Keywords

Animal diet managementdiscriminant analysislipidomicsmass spectrometry
Type
Research Article
Information
Journal of Dairy Research , Volume 88 , Issue 3 , August 2021 , pp. 261 - 264
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

Addis, M, Cabiddu, A, Pinna, G, Decandia, M, Piredda, G, Pirisi, A and Molle, G 2005. Milk and cheese fatty acid composition in sheep fed Mediterranean forages with reference to conjugated linoleic acid Cis-9, Trans-11. Journal of Dairy Science 88: 3443–3454.CrossRefGoogle ScholarPubMed
Argov-Argaman, N, Glasser, T, Muklada, H, Hadaya, O, Mesilati-Stahy, R, Raz, C and Yan Landau, S (2021) Lipidome changes, with a focus on phospholipids, due to feeding systems and processing in goat milk. Food Chemistry 340, 127938.10.1016/j.foodchem.2020.127938CrossRefGoogle ScholarPubMed
Buccioni, A, Decandia, M, Minieri, S, Molle, G and Cabiddu, A (2012) Lipid metabolism in the rumen: new insights on lipolysis and biohydrogenation with an emphasis on the role of endogenous plant factors. Animal Feed Science and Technology 174, 1–25.CrossRefGoogle Scholar
Dervishi, E, Joy, M, Sanz, A, Alvarez-Rodriguez, J, Molino, F and Calvo, JH (2012) Forage preservation (grazing vs. hay) fed to ewes affects the fatty acid profile of milk and CPT1b gene expression in the sheep mammary gland. BMC Veterinary Research 8, 106.CrossRefGoogle ScholarPubMed
Leroux, C, Le Provost, F, Petti, E, Bernard, L, Chilliard, Y and Martin, P (2003) Real-time RT-PCR and CDNA macroarray to study the impact of the genetic polymorphism at the αS1-casein locus on the expression of genes in the goat mammary gland during lactation. Reproduction Nutrition Development 43, 459–469.10.1051/rnd:2003032CrossRefGoogle Scholar
Liu, Z, Ezernieks, V, Wang, J, Wanni Arachchillage, N, Garner, JB, Wales, WJ, Cocks, BG and Rochfort, S (2017) Heat stress in dairy cattle alters lipid composition of milk. Scientific Reports 7, 1–10.Google ScholarPubMed
Manis, C, Scano, P, Nudda, A, Carta, S and Pulina, G (2021) LC-QTOF/MS untargeted metabolomics of sheep milk under cocoa husks enriched diet. Dairy 2, 112–121.CrossRefGoogle Scholar
Molle, G, Cabiddu, A, Decandia, M, Acciaro, M, Scanu, G, Addis, M, Fiori, M and Caredda, M (2021) A note on the tracing of herbage contribution to grazing sheep diet using milk and feces biomarkers. Frontiers in Veterinary Science 8, 623784.CrossRefGoogle ScholarPubMed
Morand-Fehr, P, Fedele, V, Decandia, M and Le Frileux, Y (2007) Influence of farming and feeding systems on composition and quality of goat and sheep milk. Small Ruminant Research 68, 20–34.CrossRefGoogle Scholar
Renna, M, Ferlay, A, Lussiana, C, Bany, D, Graulet, B, Wyss, U, Enri, SR, Battaglini, LM and Coppa, M (2020) Relative hierarchy of farming practices affecting the fatty acid composition of permanent grasslands and of the derived bulk milk. Animal Feed Science and Technology 267, 114561.CrossRefGoogle Scholar
Scano, P (2019) Effects of season on ovine milk composition. Journal of Dairy Research & Technology 2, 1–6.CrossRefGoogle Scholar
Schönfeldt, HC, Hall, NG and Smit, LE (2012) The need for country specific composition data on milk. Food Research International 47, 207–209.CrossRefGoogle Scholar
Tautenhahn, R, Patti, GJ, Rinehart, D and Siuzdak, G (2012) XCMS Online: a web-based platform to process untargeted metabolomic data. Analytical Chemistry 84, 5035–5039.CrossRefGoogle ScholarPubMed
Villalba, JJ and Manteca, X (2019) A case for eustress in grazing animals. Frontiers in Veterinary Science 6, 1–9.CrossRefGoogle ScholarPubMed
Supplementary material: PDF

Manis et al. supplementary material

Figure S1 and Table S1

Download Manis et al. supplementary material(PDF)
PDF 166.9 KB