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Ultrasound effects on the assembly of casein micelles in reconstituted skim milk

Published online by Cambridge University Press:  19 December 2013

Zheng Liu ,
Pablo Juliano ,
Roderick PW Williams ,
Julie Niere  and
Mary Ann Augustin
Zheng Liu
Affiliation:
CSIRO Animal, Food and Health Sciences, 671 Sneydes Road, Werribee, Victoria 3030, Australia School of Applied Sciences, College of Science, Engineering and Health, RMIT University, Victoria 3001, Australia
Pablo Juliano
Affiliation:
CSIRO Animal, Food and Health Sciences, 671 Sneydes Road, Werribee, Victoria 3030, Australia
Roderick PW Williams
Affiliation:
CSIRO Animal, Food and Health Sciences, 671 Sneydes Road, Werribee, Victoria 3030, Australia
Julie Niere
Affiliation:
School of Applied Sciences, College of Science, Engineering and Health, RMIT University, Victoria 3001, Australia
Mary Ann Augustin*
Affiliation:
CSIRO Animal, Food and Health Sciences, 671 Sneydes Road, Werribee, Victoria 3030, Australia
*
*For correspondence; e-mail: [email protected]
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Abstract

Reconstituted skim milks (10 % w/w total solids, pH 6·7–8·0) were ultrasonicated (20, 400 or 1600 kHz at a specific energy input of 286 kJ/kg) at a bulk milk temperature of <30 °C. Application of ultrasound to milk at different pH altered the assembly of the casein micelle in milk, with greater effects at higher pH and lower frequency. Low frequency ultrasound caused greater disruption of casein micelles causing release of protein from the micellar to the serum phase than high frequency. The released protein re-associated to form aggregates of smaller size but with surface charge similar to the casein micelles in the original milk. Ultrasound may be used as a physical intervention to alter the size of the micelles and the partitioning of caseins between the micellar and serum phases in milk. The altered protein equilibria induced by ultrasound treatment may have potential for the development of milk with novel functionality.

Keywords

Ultrasoundmilkcasein micelleparticle sizezeta-potentialsoluble aggregates
Type
Research Article
Information
Journal of Dairy Research , Volume 81 , Issue 2 , May 2014 , pp. 146 - 155
Copyright
Copyright © Proprietors of Journal of Dairy Research 2013 

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