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Tributyrate as a substrate for the determination of lipase activity in milk

Published online by Cambridge University Press:  01 June 2009

Helge B. Castberg ,
P. Solberg  and
T. Egelrud
Helge B. Castberg
Affiliation:
Dairy Research Institute, Chemistry and Bacteriology Branch, Agricultural University of Norway
P. Solberg
Affiliation:
Dairy Research Institute, Chemistry and Bacteriology Branch, Agricultural University of Norway
T. Egelrud
Affiliation:
Department of Chemistry, Section on Physiological Chemistry, University of Umeå, Sweden
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Summary

A procedure is described for the determination of the lipolytic activity in milk using tributyrate as substrate. The method is based on the titration of liberated butyric acid in a diethyl ether: light petroleum (2·75:1, v/v) extract of the incubation mixture. Some of the fundamental factors involved in the procedure are discussed.

Type
Research Article
Information
Journal of Dairy Research , Volume 42 , Issue 2 , June 1975 , pp. 247 - 253
Copyright
Copyright © Proprietors of Journal of Dairy Research 1975

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References

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