Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-26T07:49:21.537Z Has data issue: false hasContentIssue false
  • English
  • Français

Transduction in Streptococcus lactis

Published online by Cambridge University Press:  01 June 2009

Lois K. Allen ,
W. E. Sandine  and
P. R. Elliker
Lois K. Allen
Affiliation:
Oregon Agricultural Experiment Station, Corvallis, Oregon, U. S. A.*
W. E. Sandine
Affiliation:
Oregon Agricultural Experiment Station, Corvallis, Oregon, U. S. A.*
P. R. Elliker
Affiliation:
Oregon Agricultural Experiment Station, Corvallis, Oregon, U. S. A.*
Get access Rights & Permissions [Opens in a new window]

Summary

Transduction experiments were carried out using recipient strain Streptococcus lactis C2Ss (streptomycin-sensitive) and virulent phages prepared on donor strain Str. lactis C2Sr (streptomycin-resistant). At a high multiplicity of infection of 10, transduction to streptomycin resistance occurred at a frequency approximately 1 in 30 surviving cells and the recombinant character exhibited a delayed expression. Transduction did not occur when phages propagated on Str. lactis C2Ss were used or when phage antiserum was added to the transducing phage suspension. The transducing phage manifested an altered lytic cycle following passage through the Str. lactis C2Sr donor which rendered it incapable of reinfecting the donor but did not alter its virulence for the Str. lactis C2Ss recipient.

Type
Original Articles
Information
Journal of Dairy Research , Volume 30 , Issue 3 , October 1963 , pp. 351 - 357
Copyright
Copyright © Proprietors of Journal of Dairy Research 1963

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Adams, N. H. (1959). Bacteriophages, 1st edn. New York: Interscience Publishers, Inc.CrossRefGoogle Scholar
Brown, W. C. (1962). Thesis, Oregon State University, Corvallis, Oregon.Google Scholar
Elliker, P. R., Anderson, A. W. & Hannesson, G. (1956). J. Dairy Sci. 39, 1611.Google Scholar
Freeman, V. J. (1951). J. Bact. 61, 675.CrossRefGoogle Scholar
Groman, N. B. (1960). Microbial Genetics, p. 41. Oregon State University Press.Google Scholar
Jacob, F. & Wollman, E. L. (1961). Sexuality and the Genetics of Bacteria, 1st edn. London: Academic Press.Google Scholar
Lederberg, J. & Lederberg, E. M. (1951). J. Bact. 63, 399.Google Scholar
Lennox, E. S. (1955). Virology, 1, 190.Google Scholar
Møller-Madsen, A. (1962). 16th Int. Dairy Congr. 2, 238.Google Scholar
Morse, M. L. (1959). Proc. Nat. Acad. Sci., Wash., 45, 722.Google Scholar
Reiter, B. (1949). Nature, Lond., 164, 667.Google Scholar
Ritz, H. L. & Baldwin, J. N. (1958). Bad. Proc. p. 40.Google Scholar
Sandine, W. E., Elliker, P. R., Allen, Lois K. & Brown, W. C. (1962). J. Dairy Sci. 45, 1266.Google Scholar
Thorne, C. B. (1961). J. Bact. 83, 106.Google Scholar
United States Department Of Agriculture (1962). Publ. No. CA-E-30. Washington, D.C..Google Scholar