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Transcriptomic analysis reveals that prolactin modulates the β-casein synthesis mechanism in bovine mammary epithelial cells

Published online by Cambridge University Press:  07 November 2024

Yanying Zhang ,
Ziru Xu ,
Xiaoxue Wang ,
Xiaoming Hou ,
Yang Yang ,
Ying Liu  and
Ye Lin
Yanying Zhang
Affiliation:
Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, China
Ziru Xu
Affiliation:
Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, China
Xiaoxue Wang
Affiliation:
Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, China
Xiaoming Hou
Affiliation:
Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, China
Yang Yang
Affiliation:
Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, China
Ying Liu*
Affiliation:
Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, China
Ye Lin*
Affiliation:
Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, China Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin 150030, China
*
Authors for correspondence: Ye Lin E-Mail: [email protected] Ying Liu E-Mail: [email protected]
Authors for correspondence: Ye Lin E-Mail: [email protected] Ying Liu E-Mail: [email protected]
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Abstract

This research communication screened and identified differentiated expressed genes in bovine mammary epithelial cells (BMECs) upon prolactin (PRL) stimulation. PRL of 5 μg/ml increased β-casein synthesis in BMECs with milk protein synthesis capacity. RNA sequencing (RNA-seq) was used to screen differentially expressed genes (DEGs). A total of 375 DEGs (165 up-regulated and 210 down-regulated) were identified between PRL-stimulated group and the control group. Gene ontology enrichment analysis showed that the up-regulated genes were primarily associated with cell functions, metabolic processes, and biological regulatory processes. Pathway enrichment analysis showed that the up-regulated genes were mainly enriched in JAK-STAT, Rap1, Ras and Notch signaling pathways, which are widely involved in cell proliferation, differentiation and milk component synthesis. This study provides an initial understanding of the changes in gene expression in BMECs with PRL-stimulation, as determined by RNA-seq transcriptomic analysis, thereby enhancing our knowledge of the molecular regulation of lactation metabolism.

Keywords

Bovine mammary epithelial celldifferentially expressed genesprolactinRNA-seq
Type
Research Article
Information
Journal of Dairy Research , Volume 91 , Issue 3 , August 2024 , pp. 319 - 321
Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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