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Synbiotic effect of Lactobacillus helveticus M92 and prebiotics on the intestinal microflora and immune system of mice

Published online by Cambridge University Press:  05 January 2009

Jadranka Frece ,
Blaženka Kos ,
Ivan Krešimir Svetec ,
Zoran Zgaga ,
Jasna Beganović ,
Andreja Leboš  and
Jagoda Šušković
Jadranka Frece*
Affiliation:
Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia
Blaženka Kos
Affiliation:
Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia
Ivan Krešimir Svetec
Affiliation:
Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia
Zoran Zgaga
Affiliation:
Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia
Jasna Beganović
Affiliation:
Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia
Andreja Leboš
Affiliation:
Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia
Jagoda Šušković
Affiliation:
Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia
*
*For correspondence; e-mail: [email protected]
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Abstract

The synbiotic effect of the oral treatment of Swiss albino mice with milk-based diets supplemented with Lactobacillus helveticus M92 and various kinds of prebiotics was investigated. Survival, competition, adhesion and colonization, as well as, immunomodulating capability of Lb. helveticus M92, in synbiotic combination, in the gastrointestinal tract (GIT) of mice, were monitored. After the mice were fed with synbiotics, the lactic acid bacteria (LAB) counts in faeces were increased and reduction of enterobacteria and sulphite-reducing clostridia was observed. Similar results were obtained in homogenates of small and large intestine of mice on the 1st and 14th day, after feeding with synbiotics. After the mice were orally given viable Lb. helveticus M92 cells, alone or in combination with prebiotic, the concentration of faecal SIgA and total serum IgA antibodies from all immunized mice were higher compared with the control. The specific humoral immune response was not evoked after oral administration, therefore their synbiotic application is suitable. Among inulin, lactulose and raffinose, Lb. helveticus M92 in combination with inulin, has shown the best synbiotic effect on intestinal and faecal microflora and immune system of mice.

Keywords

Lactobacillus helveticusintestinal microfloraprobioticprebioticsynbioticmice
Type
Research Article
Information
Journal of Dairy Research , Volume 76 , Issue 1 , February 2009 , pp. 98 - 104
Copyright
Copyright © Proprietors of Journal of Dairy Research 2009

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