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Significant genetic effects of JAK2 and DGAT1 mutations on milk fat content and mastitis resistance in Holsteins

Published online by Cambridge University Press:  29 November 2019

Muhammad Zahoor Khan ,
Di Wang ,
Lei Liu ,
Tahir Usman ,
Hui Wen ,
Ruiqiang Zhang ,
Shuli Liu ,
Liangyu Shi ,
Siyuan Mi  and
Wei Xiao
...Show all authors
Muhammad Zahoor Khan
Affiliation:
Key Laboratory of Agricultural Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District, 100193Beijing, P. R. China
Di Wang
Affiliation:
Key Laboratory of Agricultural Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District, 100193Beijing, P. R. China
Lei Liu
Affiliation:
Key Laboratory of Agricultural Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District, 100193Beijing, P. R. China
Tahir Usman
Affiliation:
College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University, Mardan, Pakistan
Hui Wen
Affiliation:
Key Laboratory of Agricultural Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District, 100193Beijing, P. R. China
Ruiqiang Zhang
Affiliation:
Key Laboratory of Agricultural Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District, 100193Beijing, P. R. China
Shuli Liu
Affiliation:
Key Laboratory of Agricultural Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District, 100193Beijing, P. R. China
Liangyu Shi
Affiliation:
Key Laboratory of Agricultural Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District, 100193Beijing, P. R. China
Siyuan Mi
Affiliation:
Key Laboratory of Agricultural Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Yuan Ming Yuan West Road No. 2, Haidian District, 100193Beijing, P. R. China
Wei Xiao
Affiliation:
Beijing Animal Husbandry Station, Beijing100029, P. R. China
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Abstract

Improving the production traits and resistance against mastitis in dairy cattle is a challenge for animal scientists across the globe. The present study was designed to investigate the genetic effects of single nucleotide polymorphisms (SNPs) in Janus kinase 2 (JAK2) and diacylglycerol acyltransferase (DGAT1) genes with production and mastitis-related traits. Four SNPs in JAK2 and one in DGAT1 were analyzed through Chinese Cow's SNPs Chip-I (CCSC-I) and genotyped in a population of 312 Chinese Holsteins. Our findings demonstrated that milk fat percentage, somatic cell count (SCC), somatic cell score (SCS), serum cytokines interleukin 6 (IL-6) and interferon gamma (IFN-γ) showed significant associations (P < 0.05) with at least one or more identified SNPs. Consequently, the analysis based on haplotypes amongst the SNPs in JAK2 revealed noteworthy (P < 0.05) association with SCC and IL-6. Collectively, our results verified the pleiotropic ability of detected SNPs in bovine JAK2 and DGAT1 for milk fat percentage as well as mastitis-related traits. The significant SNPs in both the genes could serve as powerful genetic markers to minimize mastitis risk. In addition, besides SCC and SCS, the IFN-γ and IL-6 could also be used as indicators of improved genetic resistance against mastitis.

Keywords

Dairy cattleDGAT1JAK2mastitis resistance traitsmilk fatSNPs
Type
Research Article
Information
Journal of Dairy Research , Volume 86 , Issue 4 , November 2019 , pp. 388 - 393
Copyright
Copyright © Hannah Dairy Research Foundation 2019

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