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Response surface optimization of the cultivation conditions and medium components for the production of folate by Streptococcus thermophilus

Published online by Cambridge University Press:  19 May 2010

Ramya Iyer ,
Sudhir Kumar Tomar  and
Ashish Kumar Singh
Ramya Iyer
Affiliation:
Dairy Microbiology Division, National Dairy Research Institute, Karnal, India
Sudhir Kumar Tomar*
Affiliation:
Dairy Microbiology Division, National Dairy Research Institute, Karnal, India
Ashish Kumar Singh
Affiliation:
Dairy Technology Division, National Dairy Research Institute, Karnal, India
*
*For correspondence; e-mail: [email protected]
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Abstract

Optimization of the cultivation conditions and medium components for folate production by the highest folate producing Streptococcus thermophilus strain RD102 was carried out using a 23 central composite design and surface modelling method of response surface methodology. As folate production was observed to be growth-associated, hence the desired responses selected for the optimization were both folate production and growth. The selected factors based on preliminary investigations were incubation period, concentrations of p-amino benzoic acid (PABA) the growth precursor, and lactose as carbon source. The optimum concentrations of PABA and lactose were found to be 300 μm and 3% respectively at 72 h incubation. The optimized conditions resulted in an increase in folate production by 26% compared with control conditions (0% PABA and lactose at 37°C). Using the method of experimental factorial design and response surface analysis, it was possible to determine optimal operating conditions to obtain a higher folate production by Strep. thermophilus. Therefore, this study constitutes a step in developing strategies to modulate the folate level to a higher level.

Keywords

folateStreptococcus thermophilusresponse surface methodologyp-amino benzoic acidlactoseincubation period
Type
Research Article
Information
Journal of Dairy Research , Volume 77 , Issue 3 , August 2010 , pp. 350 - 356
Copyright
Copyright © Proprietors of Journal of Dairy Research 2010

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References

Bazaraa, WA & Hassan, EE 1996 Response surface optimization for the continuous glucose isomerization process. Journal of Industrial Microbiology 17 100–103CrossRefGoogle Scholar
Bongers, RS, Hoefnagel, MHN, Starrenburg, MJC, Siemerink, MAJ, Arends, JGA, Hugenholtz, J & Kleerebezem, M 2003 IS981-mediated adaptive evolution recovers lactate production by ldhB transcription activation in a lactate dehydrogenase-deficient strain of Lc. lactis. Journal of Bacteriology 185 4499–4507CrossRefGoogle Scholar
Crittenden, RG, Martinez, NR & Playne, MJ 2003 Synthesis and utilization of folate by yogurt starter cultures and probiotic bacteria. International Journal of Food Microbiology 80 217–222CrossRefGoogle ScholarPubMed
Dagbagli, S & Goksungur, Y 2008 Optimization of β-galactosidase production using Kluyveromyces lactis NRRL Y-8279 by response surface methodology. Process Biotechnology 11(4) 1–12Google Scholar
Duwat, P, Sourice, S, Cesselin, B, Lamberet, G, Vido, K, Gaudu, P, Le Loir, Y, Violet, F, Loubiere, P & Gruss, A 2001 Respiration capacity of the fermenting bacterium Lc. lactis and its positive effects on growth and survival. Journal of Bacteriology 183 4509–4516CrossRefGoogle Scholar
FAO/WHO 2002 Report of a joint FAO/WHO expert consultation. In: Human vitamin and mineral requirements, Thailand p. 290Google Scholar
Holasová, M, Fiedlerová, V, Roubal, P & Pechacova, M 2004 Biosynthesis of folates by lactic acid bacteria and propionibacteria in fermented milk. Czechoslovakian Journal of Food Science 22 175–181CrossRefGoogle Scholar
Hyun, TH & Tamura, T 2005 Trienzyme Extraction in Combination with Microbiologic Assay in Food Folate Analysis: An Updated Review. Experimental Biology and Medicine 230 444–454CrossRefGoogle ScholarPubMed
Iyer, R, Tomar, SK, Singh, R & Sharma, R 2009 Estimation of folate in milk by microbiological assay using trienzyme extraction method. Milchwissenschaft 64(2) 125–127Google Scholar
Keagy, PM 1985 Folacin-microbiological and animal assay. In Augustin, J, Klein, BP, Becker, D & Venugopal, PB (Eds.). Methods of vitamin assay. New York: Wiley p. 445–463Google Scholar
Kim, JK, Oh, BR, Shin, H, Eom, C & Kim, SW 2008 Statistical optimization of enzymatic saccharification and ethanol fermentation using food waste. Process Biochemistry 43(11) 1308–1312CrossRefGoogle Scholar
LeBlanc, JG, Giori, GSD, Smid, EJ, Hugenholtz, J & Sesma, F 2007 Folate production by lactic acid bacteria and other food-grade microorganisms. Communicating Current Research and Educational Topics and Trends in Applied Microbiology 329–339Google Scholar
Li, C, Bai, J, Cai, Z & Ouyang, F 2002 Optimization of a cultural medium for bacteriocin production by Lc. lactis using a response surface methodology. Journal of Biotechnology 93 27–34CrossRefGoogle Scholar
Lin, MY & Young, CM 2000 Folate levels in cultures of lactic acid bacteria. International Dairy Journal 10 409–414CrossRefGoogle Scholar
Liu, CQ, Chen, QH, Tang, B, Ruan, H & He, GQ 2007 Response surface methodology for optimizing the fermentation medium of alpha-galactosidase in solid-state fermentation. Letters in Applied Microbiology 45(2) 206–212CrossRefGoogle ScholarPubMed
Montgomery, DC 1997 Response surface methods and other approaches to process optimization. In: Montgomery, DC (Ed.) Design and analysis of experiments. New York: John Wiley and Sons p. 427–510Google Scholar
Rodrigues, LR, Teixeira, J, Oliveira, R & van der Mei, HC 2006 Response surface optimization of the medium components for the production of biosurfactants by probiotic bacteria. Process Biochemistry 41 1–10CrossRefGoogle Scholar
Siegumfeldt, H, Rechinger, KB & Jakobsen, M 2000 Dynamic changes of intracellular pH in individual lactic acid bacterium cells in response to a rapid drop in extracellular pH. Applied Environmental Microbiology 66 2330–2335CrossRefGoogle ScholarPubMed
Smid, EJ, Starrenburg, MJC, Mierau, I, Sybesma, W & Hugenholtz, J 2001 Increase of folate levels in fermented foods. Innovations in Food Technology 10 13–15Google Scholar
Sybesma, W, Starrenburg, M, Tijsseling, L, Hoefnagel, MHN & Hugenholtz, J 2003 Effects of cultivation conditions on folate production by lactic acid bacteria. Applied Environmental Microbiology 69 4542–4548CrossRefGoogle ScholarPubMed
Tomar, SK, Srivatsa, N, Iyer, R & Singh, R 2009 Estimation of Folate Production by Strep. themophilus using Modified Microbiological Assay. Milchwissenshaft 64(3) 260–263Google Scholar
Vohra, A & Satyanarayana, T 2002 Statistical optimization of the medium components by response surface methodology to enhance phytase production by Pichia anomala. Process Biochemistry 37 999–1004.CrossRefGoogle Scholar
Wang, YH, Feng, JT, Zhang, Q & Zhang, X 2008 Optimization of fermentation condition for antibiotic production by Xenorhabdus nematophila with response surface methodology. Journal of Applied Microbiology 104(3) 735–744CrossRefGoogle ScholarPubMed
Wegkamp, A 2008 Modulation of Folate Production in Lactic Acid Bacteria. The Netherlands: Wageningen University. Ph.D. thesis p. 53–73Google Scholar
Wright, J, Dainty, J & Finglas, P 2007 Folic acid metabolism in human subjects revisited: potential implications for proposed mandatory folic acid fortification in the UK. British Journal of Nutrition 98 665–666CrossRefGoogle ScholarPubMed