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Relationship between the level of N-acetyl-β-D-glucosaminidase (NAGase) in bovine milk and the presence of mastitis pathogens

Published online by Cambridge University Press:  01 June 2009

Barry J. Kitchen ,
W. Seng Kwee ,
Gordon Middleton  and
R. Jeffrey Andrews
Barry J. Kitchen
Affiliation:
Otto Madsen Dairy Research Laboratory, Department of Primary Industries, Hamilton, Brisbane 4007, Australia
W. Seng Kwee
Affiliation:
Otto Madsen Dairy Research Laboratory, Department of Primary Industries, Hamilton, Brisbane 4007, Australia
Gordon Middleton
Affiliation:
Otto Madsen Dairy Research Laboratory, Department of Primary Industries, Hamilton, Brisbane 4007, Australia
R. Jeffrey Andrews
Affiliation:
Otto Madsen Dairy Research Laboratory, Department of Primary Industries, Hamilton, Brisbane 4007, Australia
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Summary

Changes in the level of the tissue damage marker enzyme, N-acetyl-β-D-glucosaminidase (NAGase) in quarter fore milks were found to be related to the presence and types of pathogenic bacteria present and to somatic cell counts (SCC). Minor pathogens (coagulase-negative staphylococci, Corynebacterium bovis) elicited a mild SCC increase (from a mean of 243 × 103/ml in healthy quarters to 504 × 103/ml in infected quarters) with marginal tissue damage (mean NAGase activity increased from 21 in healthy quarters to 28 in infected quarters). Major pathogens (i.e. Staphylococcus aureus, Streptococcus agalactiae, Str. dysgalactiae and Str. uberis) caused more severe tissue damage (mean NAGase of 48) and SCC increases (mean, 2803 × 103/ml). The NAGase test could also be used effectively on composite milk samples where regular monthly NAGase analysis was able to identify correctly 74% of animals having infected quarters. The possibility of combining SCC and NAGase data in order to give a more definite diagnosis of bovine mastitis is discussed.

Type
Original Articles
Information
Journal of Dairy Research , Volume 51 , Issue 1 , February 1984 , pp. 11 - 16
Copyright
Copyright © Proprietors of Journal of Dairy Research 1984

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References

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