Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-23T04:34:24.478Z Has data issue: false hasContentIssue false
  • English
  • Français

Relationship between somatic cell count, polymorphonuclear leucocyte count and quality parameters in bovine bulk tank milk

Published online by Cambridge University Press:  13 March 2009

Erik Wickström ,
Karin Persson-Waller ,
Helena Lindmark-Månsson ,
Karin Östensson  and
Åse Sternesjö
Erik Wickström
Affiliation:
Department of Food Science, Swedish University of Agricultural Sciences, PO Box 7051, SE-750 07 Uppsala, Sweden
Karin Persson-Waller
Affiliation:
Department of Clinical Sciences, Swedish University of Agricultural Sciences, PO Box 7054, SE-750 07 Uppsala, Sweden Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute, SE-751 89 Uppsala, Sweden
Helena Lindmark-Månsson
Affiliation:
Swedish Dairy Association, Ideon Science Park, SE-223 70 Lund, Sweden
Karin Östensson
Affiliation:
Department of Clinical Sciences, Swedish University of Agricultural Sciences, PO Box 7054, SE-750 07 Uppsala, Sweden
Åse Sternesjö*
Affiliation:
Department of Food Science, Swedish University of Agricultural Sciences, PO Box 7051, SE-750 07 Uppsala, Sweden
*
*For correspondence; e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The somatic cell count (SCC) in bovine bulk tank milk is presently used as an indicator of raw milk quality, reflecting the udder health status of the herd. During mastitis, SCC increases, mostly owing to an influx of polymorphonuclear leucocytes (PMN) from blood into milk, with a concomitant change in milk composition. Bulk tank milk samples were categorized according to their SCC, as well as polymorphonuclear leucocyte count (PMNC), to study relationships between SCC, PMNC and various raw milk quality traits, i.e. contents of total protein, whey protein, casein, fat and lactose, casein number, proteolysis and rheological properties. The proportion of PMN, obtained by direct microscopy, was significantly higher in samples with high SCC compared with low SCC samples. SCC and PMNC were strongly correlated, yielding a correlation coefficient of 0·85. High SCC samples had lower lactose and casein contents, lower casein number and more proteolysis than low SCC samples. Samples with high PMNC had a lower casein number than low PMNC samples. Samples with high and low SCC or PMNC did not differ in respect to rheological properties. Our results do not indicate that PMNC is a better biomarker than SCC for raw bulk tank milk quality, as previously proposed.

Keywords

Neutrophilsmilk compositionbulk tank milk proteolysis
Type
Research Article
Information
Journal of Dairy Research , Volume 76 , Issue 2 , May 2009 , pp. 195 - 201
Copyright
Copyright © Proprietors of Journal of Dairy Research 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Åkerstedt, M, Waller, KP, Larsen, LB, Forsbäck, L & Sternesjö, Å 2008 Relationship between haptoglobin and serum amyloid A in milk and milk quality. International Dairy Journal 18 669–674CrossRefGoogle Scholar
Auldist, MJ & Hubble, IB 1998 Effects of mastitis on raw milk and dairy products. Australian Journal of Dairy Technology 53 28–36Google Scholar
Azzara, CD & Dimick, PS 1985 Lipoprotein lipase activity of milk from cows with prolonged subclinical mastitis. Journal of Dairy Science 68 3171–3175CrossRefGoogle ScholarPubMed
Ballou, LU, Pasquini, M, Bremel, RD, Everson, T & Sommer, D 1995 Factors affecting herd milk composition and milk plasmin at four levels of somatic cell counts. Journal of Dairy Science 78 2186–2195CrossRefGoogle ScholarPubMed
Barbano, DM, Ma, Y & Santos, MV 2006 Influence of raw milk quality on fluid milk shelf life. Journal of Dairy Science 89 E15–E19CrossRefGoogle ScholarPubMed
Bastian, ED & Brown, RJ 1996 Plasmin in milk and dairy products: An update. International Dairy Journal 6 435–457CrossRefGoogle Scholar
Berning, LM & Shook, GE 1992 Prediction of mastitis using milk somatic cell count, N-acetyl-(beta)-D-glucosaminidase, and lactose. Journal of Dairy Science 75 1840–1848CrossRefGoogle ScholarPubMed
Caroprese, M, Marzano, A, Schena, L, Marino, R, Santillo, A & Albenzio, M 2007 Contribution of macrophages to proteolysis and plasmin activity in ewe bulk milk. Journal of Dairy Science 90 2767–2772CrossRefGoogle ScholarPubMed
Dosogne, H, Vangroenweghe, F, Mehrzad, J, Massart-Leën, AM & Burvenich, C 2003 Differential leukocyte count method for bovine low somatic cell count milk. Journal of Dairy Science 86 828–834CrossRefGoogle ScholarPubMed
Gargouri, A, Hamed, H & El Feki, A 2008 Total and differential bulk cow milk somatic cell counts and their relation with lipolysis. Livestock Science 113 274–279CrossRefGoogle Scholar
Haddadi, K, Prin-Mathieu, C, Moussaoui, F, Faure, GC, Vangroenweghe, F, Burvenich, C & Le Roux, Y 2006 Polymorphonuclear neutrophils and Escherichia coli proteases involved in proteolysis of casein during experimental E. coli mastitis. International Dairy Journal 16 639–647CrossRefGoogle Scholar
Hallén, E, Allmere, T, Naslund, J, Andren, A & Lunden, A 2007 Effect of genetic polymorphism of milk proteins on rheology of chymosin-induced milk gels. International Dairy Journal 17 791–799CrossRefGoogle Scholar
Harayani, S, Datta, N, Elliott, AJ & Deeth, HC 2003 Production of proteinases by psychrotrophic bacteria in raw milk stored at low temperature. Australian Journal of Dairy Technology 58 15–20Google Scholar
Harmon, RJ 1994 Symposium: Mastitis and genetic evaluation for somatic-cell count—physiology of mastitis and factors affecting somatic-cell counts. Journal of Dairy Science 77 2103–2112CrossRefGoogle Scholar
Hurley, MJ, Larsen, LB, Kelly, AL & McSweeney, PLH 2000 The milk acid proteinase cathepsin D: a review. International Dairy Journal 10 673–681CrossRefGoogle Scholar
International Dairy Federation (IDF) 2006 Milk-enumeration of somatic cells-part 1. Microscopic method (reference method). IDF Standard 148-1, International Dairy Federation, Brussels.Google Scholar
Kehrli, ME Jr & Shuster, DE 1994 Factors affecting milk somatic cells and their role in health of the bovine mammary gland. Journal of Dairy Science 77 619–627CrossRefGoogle ScholarPubMed
Kelly, AL, O'Flaherty, F & Fox, PF 2006 Indigenous proteolytic enzymes in milk: A brief overview of the present state of knowledge. International Dairy Journal 16 563–572CrossRefGoogle Scholar
Kelly, AL, Tiernan, D, O'Sullivan, C & Joyce, P 2000 Correlation between bovine milk somatic cell count and polymorphonuclear leucocyte level for samples of bulk milk and milk from individual cows. Journal of Dairy Science 83 300–304CrossRefGoogle ScholarPubMed
Larsen, LB, Rasmussen, MD, Bjerring, M & Nielsen, JH 2004 Proteases and protein degradation in milk from cows infected with Streptococcus uberis. International Dairy Journal 14 899–907CrossRefGoogle Scholar
Le, TX, Datta, N & Deeth, HC 2006 A sensitive method for measuring bacterial proteolysis and proteinase activity in UHT milk. Food Research International 39 823–830CrossRefGoogle Scholar
Le Roux, Y 2003 Polymorphonuclear proteolytic activity and milk composition change. Veterinary Research 34 629–645CrossRefGoogle ScholarPubMed
Leitner, G, Krifucks, O, Merin, U, Lavi, Y & Silanikove, N 2006 Interactions between bacteria type, proteolysis of casein and physico-chemical properties of bovine milk. International Dairy Journal 16 648–654CrossRefGoogle Scholar
Leitner, G, Silanikove, N, Jacobi, S, Weisblit, L, Bernstein, S & Merin, U 2008 The influence of storage on the farm and in dairy silos on milk quality for cheese production. International Dairy Journal 18 109–113CrossRefGoogle Scholar
Mehrzad, J, Desrosiers, C, Lauzon, K, Robitaille, G, Zhao, X & Lacasse, P 2005 Proteases involved in mammary tissue damage during endotoxin-induced mastitis in dairy cows. Journal of Dairy Science 88 211–222CrossRefGoogle ScholarPubMed
O'Brien, B, Fitzpatrick, C, Meaney, WJ & Joyce, PM 2003 Relationship between somatic cell count and neutrophils in milk. Mastitis Newsletter 25 13Google Scholar
O'Sullivan, CA, Joyce, PJ, Sloan, T & Shattock, AG 1992 Capture immunoassay for the diagnosis of bovine mastitis using a monoclonal antibody to polymorphonuclear granulocytes. Journal of Dairy Research 59 123–133CrossRefGoogle ScholarPubMed
Paape, MJ, Bannerman, DD, Zhao, X & Lee, JW 2003 The bovine neutrophil: structure and function in blood and milk. Veterinary Research 34 597–627Google ScholarPubMed
Pillai, SR, Kunze, E, Sordillo, LM & Jayarao, BM 2001 Application of differential inflammatory cell count as a tool to monitor udder health. Journal of Dairy Science 84 1413–1420CrossRefGoogle ScholarPubMed
Politis, I 1996 Plasminogen activator system: implications for mammary cell growth and involution. Journal of Dairy Science 79 1079–1107CrossRefGoogle ScholarPubMed
Politis, I & Ng-Kwai-Hang, KF 1988 Association between somatic cell count of milk and cheese-yielding capacity. Journal of Dairy Science 71 1720–1727CrossRefGoogle Scholar
Pyörälä, S 2003 Indicators of inflammation in the diagnosis of mastitis. Veterinary Research 34 565–578CrossRefGoogle Scholar
Saad, AM & Östensson, K 1990 Flow cytofluorometric studies on the alteration of leukocyte populations in blood and milk during endotoxin-induced mastitis in cows. American Journal of Veterinary Research 51 1603–1607CrossRefGoogle ScholarPubMed
Sandholm, M, Honkanen-Buzalski, T, Kaartinen, L & Pyörälä, S 1995 The bovine udder and mastitis. University of Helsinki, Faculty of Veterinary Medicine, Helsinki, FinlandGoogle Scholar
Schröder, AC & Hamann, J 2005 The influence of technical factors on differential cell count in milk. Journal of Dairy Research 72 153–158CrossRefGoogle ScholarPubMed
Schukken, YH, Wilson, DJ, Welcome, F, Garrison-Tikofsky, L & Gonzalez, RN 2003 Monitoring udder health and milk quality using somatic cell counts. Veterinary Research 34 579–596CrossRefGoogle ScholarPubMed
Shuster, DE, Harmon, RJ, Jackson, JA & Hemken, RW 1991 Suppression of milk production during endotoxin-induced mastitis. Journal of Dairy Science 74 3763–3774CrossRefGoogle ScholarPubMed
Somers, JM, O'Brien, B, Meaney, WJ & Kelly, AL 2003 Heterogeneity of proteolytic enzyme activities in milk samples of different somatic cell count. Journal of Dairy Research 70 45–50CrossRefGoogle ScholarPubMed
Vangroenweghe, F, Dosogne, H & Burvenich, C 2002 Composition and milk cell characteristics in quarter milk fractions of dairy cows with low cell count. Veterinary Journal 164 254–260CrossRefGoogle ScholarPubMed
Wiking, L, Frost, MB, Larsen, LB & Nielsen, JH 2002 Effects of storage conditions on lipolysis, proteolysis and sensory attributes in high quality raw milk. Milchwissenschaft-Milk Science International 57 190–194Google Scholar