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Pseudomonas fluorescens and Pseudomonas putida from refrigerated raw milk: genetic diversity and lipoproteolytic activity

Published online by Cambridge University Press:  11 February 2022

Rosana De Longhi ,
Selma de Souza Correia ,
Samera Rafaela Bruzaroski ,
Regina Celia Poli-Frederico ,
Rafael Fagnani  and
Elsa Helena Walter de Santana
Rosana De Longhi
Affiliation:
Universidade Pitágoras Unopar (UNOPAR), Mestrado em Ciência e Tecnologia de Leite e Derivados, Londrina, Paraná, Brazil
Selma de Souza Correia
Affiliation:
UNOPAR, Mestrado em Saúde e Produção Animal, Arapongas, Paraná, Brazil
Samera Rafaela Bruzaroski
Affiliation:
Universidade Pitágoras Unopar (UNOPAR), Mestrado em Ciência e Tecnologia de Leite e Derivados, Londrina, Paraná, Brazil
Regina Celia Poli-Frederico
Affiliation:
UNOPAR, Mestrado e Doutorado em Ciência da Reabilitação, Londrina, Paraná, Brazil
Rafael Fagnani
Affiliation:
Universidade Estadual de Londrina, Londrina, Paraná, Brazil
Elsa Helena Walter de Santana*
Affiliation:
Universidade Pitágoras Unopar (UNOPAR), Mestrado em Ciência e Tecnologia de Leite e Derivados, Londrina, Paraná, Brazil UNOPAR, Mestrado em Saúde e Produção Animal, Arapongas, Paraná, Brazil
*
Author for correspondence: Elsa Helena Walter de Santana, Email: [email protected]
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Abstract

In this research communication the genetic diversity of Pseudomonas fluorescens (n = 67) and Pseudomonas putida (n = 44) isolated from refrigerated raw milk from bulk tank trucks were verified. The relationship between the genetic profile of the isolates and their lipoproteolytic potential was evaluated using skim milk agar and tributyrin agar (21°C/72 h). The lipoproteolytic potential (low or high), evaluated by the diameter of the halos (cm), was correlated with the number of milk producing properties that contributed to each sample (one sample = one bulk tank truck; 8−80 producers/sample) and the distance between the dairy properties and the processing plant (21−370 km). P. fluorescens was confirmed in all samples, while P. putida in 60% samples. For both species, two clusters (I and II) were observed, and the first one showed lower genotypic diversity and the presence of isolates with 100% similarity. P. fluorescens isolates presenting at least 70% similarity were 83.9% in Cluster I (n = 31) and 44.4% in Cluster II. In both clusters (I and II) observed in the P. fluorescens dendrogram, the occurrence of high proteolytic and lipolytic potential were equivalent. The higher the number of farms per milk sample, the greater the lipoproteolytic intensity of P. fluorescens isolates. In relation to P. putida isolates, 74% presented at least 50% similarity in Cluster I (n = 27) and only 35% in Cluster II (n = 17). The occurrence of high proteolysis linked to P. putida was proportional between both Clusters, but the occurrence of high lipolysis was greater in Cluster II. No significant association was detected between P. putida isolates and the variables studied. The results indicate the circulation of P. putida and P. fluorescens with 100% similarity in different milk producing regions. The level of genetic diversity was related only to the lipolytic capacity of P. putida.

Keywords

EnzymespsychrotrophicqualityRep PCR
Type
Research Article
Information
Journal of Dairy Research , Volume 89 , Issue 1 , February 2022 , pp. 86 - 89
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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