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Proteomic profiling of ovine milk after grading up

Published online by Cambridge University Press:  14 May 2021

Xiaohu Su ,
Zhong Zheng ,
Liguo Zhang ,
Urhan Bai ,
Ying Ma ,
Yingjie Dou ,
Xiaoran Zhang ,
Guanghua Su ,
Ningcong Zhou  and
Guangpeng Li
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Xiaohu Su*
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China
Zhong Zheng
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China
Liguo Zhang
Affiliation:
Ulanqab Animal Husbandry Workstation, Ulanqab Agriculture and Animal Husbandry Bureau, Ulanqab, Inner Mongolia Autonomous Region, 012000, PR China
Urhan Bai
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China
Ying Ma
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China
Yingjie Dou
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China
Xiaoran Zhang
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China
Guanghua Su
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China
Ningcong Zhou
Affiliation:
Ulanqab Animal Husbandry Workstation, Ulanqab Agriculture and Animal Husbandry Bureau, Ulanqab, Inner Mongolia Autonomous Region, 012000, PR China
Guangpeng Li
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China
Li Zhang*
Affiliation:
The State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia Autonomous Region, 010018, PR China
*
Author for correspondence: Li Zhang, Email: [email protected]; Xiaohu Su, Email: [email protected]
Author for correspondence: Li Zhang, Email: [email protected]; Xiaohu Su, Email: [email protected]
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Abstract

We have previously bred Chinese local dairy sheep through grading up with local Small-Tailed Han (STH) sheep as female parent and DairyMeade (DM) sheep as male parent. In this research communication we characterize the whey protein profile of STH sheep and their offspring (F1, F2) to reveal physiological differences and variation in milk traits. A total of 1032 whey proteins were identified through tandem mass tag labeling (TMT) proteome profiling. Three proteins were significantly differentially abundant between F1 and STH milk, six between F2 and STH milk and five between F1 and F2 milk. In terms of differential changes between generations, WASHC4 and CUTA of F1 and Ig-like domain-containing protein of F2 milk were dominant whey proteins. Overall, the results showed that the whey protein profiles of different generations varied little. The crossbreeds of STH and DM sheep would be suitable for the development of the Chinese local sheep milk industry, and the F2 may be a better population for sheep milk production.

Keywords

Milkovineproteomic
Type
Research Article
Information
Journal of Dairy Research , Volume 88 , Issue 2 , May 2021 , pp. 201 - 204
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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Footnotes

*

These authors contributed equally to this project.

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