Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-26T11:03:05.491Z Has data issue: false hasContentIssue false
  • English
  • Français

Occurrence of methicillin-resistant Staphylococcus aureus (MRSA) in ‘coalho’ cheese produced in Brazil

Published online by Cambridge University Press:  21 February 2022

Felipe R. M. de Aguiar ,
Larissa Q. Rocha ,
Morgana M. O. Barboza ,
Tiago L. Sampaio ,
Nádia A. P. Nogueira  and
Ramon R. P. P. B. de Menezes
Felipe R. M. de Aguiar
Affiliation:
Department of Pharmaceutical Sciences, Federal University of Ceará, Fortaleza, Brazil
Larissa Q. Rocha
Affiliation:
Department of Pharmaceutical Sciences, Federal University of Ceará, Fortaleza, Brazil
Morgana M. O. Barboza
Affiliation:
Department of Biotechnology, Federal University of Ceará, Fortaleza, Brazil
Tiago L. Sampaio
Affiliation:
Department of Clinical and Toxicological Analysis, Federal University of Ceará, Fortaleza, Brazil
Nádia A. P. Nogueira
Affiliation:
Department of Clinical and Toxicological Analysis, Federal University of Ceará, Fortaleza, Brazil
Ramon R. P. P. B. de Menezes*
Affiliation:
Department of Clinical and Toxicological Analysis, Federal University of Ceará, Fortaleza, Brazil
*
Author for correspondence: Ramon RPPB de Menezes, Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The experiments reported in this research communication analysed the presence of methicillin-resistant Staphylococcus aureus (MRSA) in 112 samples of ‘coalho’ cheese, from 56 dairy producing farms in 28 cities in all mesoregions of the State of Ceará, Brazil. To assess antimicrobial resistance we also examined the presence of genes encoding enterotoxins and toxic shock syndrome toxin, as well as the presence of the blaZ gene for β-lactamases, and resistance to oxacillin. The research found 69 isolates of S. aureus, of which 13.04% had the mecA gene encoding the penicillin-binding protein, which confers resistance to methicillin, in cheese samples from 6 different cities. This included the state capital, Fortaleza, which had the largest prevalence (23.19%) of mecA positive isolates. It was also found that 55.07% of the isolates of S. aureus had the blaZ gene, and 7.25% demonstrated resistance to oxacillin in the plate disc diffusion tests. We did not show the presence of isolates carrying toxigenic genes. The findings suggest that strict supervision of production processes in the dairy industry is necessary in all production scale processes, thus preventing contamination and possible problems for consumers.

Keywords

Dairy productsEnterotoxinsMRSAStaphylococcus aureus
Type
Research Article
Information
Journal of Dairy Research , Volume 89 , Issue 1 , February 2022 , pp. 100 - 103
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation.

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aragão, BB, Trajano, SC, Silva, JG, Oliveira, RP, Pinheiro Júnior, JW, Peixoto, RM and Mota, RA (2019) Short communication: high frequency of β-lactam-resistant Staphylococcus aureus in artisanal coalho cheese made from goat milk produced in northeastern Brazil. Journal of Dairy Science 102, 6923–6927.CrossRefGoogle ScholarPubMed
Archer, NK, Mazaitis, MJ, Costerton, JW, Leid, JG, Powers, ME and Shirtliff, ME (2011) Staphylococcus aureus biofilms: properties, regulation, and roles in human disease. Virulence 2, 445–459.CrossRefGoogle ScholarPubMed
Brazil (2003) Ministry of Agriculture, Livestock and Supply. Normative Instruction no 62, of 26 August 2003. Official Gazette [of] the Federative Republic of Brazil 1 Section I 16–17.Google Scholar
Chen, C, Zhao, Q, Guo, J, Li, Y and Chen, Q (2017) Identification of methicillin-resistant Staphylococcus aureus (MRSA) using simultaneous detection of mecA, Nuc, and femB by loop-mediated isothermal amplification (LAMP). Current Microbiology 74, 965–971.CrossRefGoogle ScholarPubMed
Clinical Laboratory Standards Institute (2016) Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals. Approved Standard M31-A3.Google Scholar
Costa, FN, Belo, NO, Costa, EA, Andrade, GI, Pereira, LS, Carvalho, IA and Santos, RL (2018) Frequency of enterotoxins, toxic shock syndrome toxin-1, and biofilm formation genes in Staphylococcus aureus isolates from cows with mastitis in the Northeast of Brazil. Tropical Animal Health Production 50, 1089–1097.CrossRefGoogle ScholarPubMed
Danelli, T, Duarte, FC, Oliveira, TA, Silva, RS, Alfieri, DF, Gonçalves, GB, Oliveira, CF, Tavares, ER, Yamauchi, LM, Perugini, MRE and Yamada-Ogatta, SF (2020) Nasal carriage by Staphylococcus aureus among healthcare workers and students attending a university hospital in southern Brazil: prevalence, phenotypic, and molecular characteristics. Interdisciplinary Perspectives on Infectious Diseases 2020, 3808036.CrossRefGoogle ScholarPubMed
Gonzalez, AGM, Marques, LMP, Gomes, MDSA, Beltrão, JCDC, Pinheiro, MG, Esper, LMR, Paula, GR, Teixeira, LA and Aguiar-Alves, F (2017) Methicillin-resistant Staphylococcus aureus in minas frescal cheese: evaluation of classic enterotoxin genes, antimicrobial resistance and clonal diversity. FEMS Microbiology Letters 364, 1–7.CrossRefGoogle ScholarPubMed
Guimarães, FF, Manzi, MP, Joaquim, SF, Richini-Pereira, VB and Langoni, H (2017) Short communication: outbreak of methicillin-resistant Staphylococcus aureus (MRSA)-associated mastitis in a closed dairy herd. Journal of Dairy Science 100, 723–730.CrossRefGoogle Scholar
Kateete, DP, Kimani, CN, Katabazi, FA, Okeng, A, Okee, MS, Nanteza, A, Joloba, ML and Najjuka, FC (2010) Identification of Staphylococcus aureus: DNase and Mannitol salt agar improve the efficiency of the tube coagulase test. Annals of Clinical Microbiology and Antimicrobials 13 9–23.Google Scholar
McKay, AM (2008) Antimicrobial resistance and heat sensitivity of oxacillin-resistant, mecA-positive Staphylococcus spp. From unpasteurized milk. Journal of Food Protection 71, 186–190.CrossRefGoogle ScholarPubMed
Papadopoulos, P, Papadopoulos, T, Angelidis, AS, Boukouvala, E, Zdragas, A, Papa, A, Hadjichristodoulou, C and Sergelidis, D (2018) Prevalence of Staphylococcus aureus and of methicillin-resistant S. aureus (MRSA) along the production chain of dairy products in north-western Greece. Food Microbiology 69, 43–50.CrossRefGoogle ScholarPubMed
Riva, A, Borghi, E, Cirasola, D, Colmegna, S, Borgo, F, Amato, E, Pontello, MM and Morace, G (2015) Methicillin-resistant Staphylococcus aureus in raw milk: prevalence, SCCmec typing, enterotoxin characterization, and antimicrobial resistance patterns. Journal of Food Protection 78, 1142–1146.CrossRefGoogle ScholarPubMed
Silva, SS, Cidral, TA, Soares, MJ and de Melo, MC (2015) Enterotoxin-encoding genes in Staphylococcus spp. From food handlers in a university restaurant. Foodborne Pathogens and Disease 12, 921–925.CrossRefGoogle Scholar
Soares, EKB, Esmerino, EA, Ferreira, MVS, Silva, MAAP, Freitas, MQ and Cruz, AG (2017) What are the cultural effects on consumers’ perceptions? A case study covering coalho cheese in the Brazilian northeast and southeast area using word association. Food Research International 102, 553–558.CrossRefGoogle ScholarPubMed
Song, JW, Yang, SJ, Shin, S, Seo, KS, Park, YH and Park, KT (2016) Genotypic and phenotypic characterization of methicillin-resistant Staphylococcus aureus isolated from bovine mastitic milk in Korea. Journal of Food Protection 79, 1725–1732.CrossRefGoogle ScholarPubMed
Supplementary material: PDF

de Aguiar et al. supplementary material

de Aguiar et al. supplementary material

Download de Aguiar et al. supplementary material(PDF)
PDF 516.3 KB