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Negative effect of insulin-induced gene 2 on milk fat synthesis in buffalo mammary epithelial cells

Published online by Cambridge University Press:  19 January 2022

Xinyang Fan ,
Yongyun Zhang ,
Lihua Qiu  and
Yongwang Miao
Xinyang Fan
Affiliation:
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming650201, Yunnan, China
Yongyun Zhang
Affiliation:
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming650201, Yunnan, China Teaching Demonstration Center of the Basic Experiments of Agricultural Majors, Yunnan Agricultural University, Kunming650201, Yunnan, China
Lihua Qiu
Affiliation:
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming650201, Yunnan, China
Yongwang Miao*
Affiliation:
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming650201, Yunnan, China
*
Author for correspondence: Yongwang Miao, Email: [email protected]
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Abstract

Insulin-induced gene 2 (INSIG2) is a recently identified gene that is implicated in the regulation of cholesterol metabolism and lipogenesis in mammals. Although the data in goats emphasizes a role for INSIG2 in milk fat synthesis, the regulatory mechanism in buffalo is not clear. In this study, we analyzed the protein abundance of INSIG2 at peak lactation and dry-off period in buffalo mammary tissue. The results indicated that, relative to the peak lactation, the protein abundance of INSIG2 in the dry-off period was higher. To determine the function of INSIG2 in milk fat synthesis, INSIG2 was overexpressed and knocked down by lentiviral transfection in buffalo mammary epithelial cells (BuMECs). The response to overexpressing INSIG2 included down-regulation of SREBP, PPARG, FASN, ELOVL6, SCD, APGAT6 and TIP47 coupled with a decrease in content of triacylglycerol (TAG). However, in response to knockdown of INSIG2, the significant increase in content of TAG along with marked up-regulation of SREBP, PPARG, FASN, ELOVL6, SCD, APGAT6 and TIP47 suggests that INSIG2 negatively affects milk fat synthesis in BuMECs. No significant difference in mRNA abundance of GPAM and DGAT2 in response to overexpression or interference of INSIG2 indicates that they might also be influenced by other regulatory factors. Taken together, our results provide strong support for the negative effect of INSIG2 on milk fat synthesis in BuMECs.

Keywords

BuffaloINSIG2interference and overexpressionmilk fat synthesis
Type
Research Article
Information
Journal of Dairy Research , Volume 88 , Issue 4 , November 2021 , pp. 401 - 406
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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Footnotes

*

These authors contributed equally to this paper.

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