Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-06T04:15:52.578Z Has data issue: false hasContentIssue false
  • English
  • Français

A modified method for the determination of glyceride glycerol

Published online by Cambridge University Press:  01 June 2009

J. H. Moore
J. H. Moore
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading
Get access Rights & Permissions [Opens in a new window]

Summary

A routine method giving accurate results was required for the determination of the glyceride content of eluate fractions obtained in the silicic acid chromatography of lipid mixtures. The technique of determining glyceride glycerol by saponification, oxidation of the liberated glycerol to formaldehyde, reaction of the formaldehyde with chromotropic acid and measurement of the resulting chromogen at 570 mμ has been investigated. Procedures based on this principle have appeared in the literature but none of these has been found to be entirely satisfactory. Accordingly, a modification of the method has been developed that is capable of giving results of a high degree of accuracy.

Type
Original Articles
Information
Journal of Dairy Research , Volume 29 , Issue 2 , June 1962 , pp. 141 - 147
Copyright
Copyright © Proprietors of Journal of Dairy Research 1962

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Albrink, M. J. (1959). J. Lipid Res. 1, 53.CrossRefGoogle Scholar
Barron, E. J. & Hanahan, D. J. (1958). J. biol. Chem. 231, 493.CrossRefGoogle Scholar
Brown, W. D. (1959). Aust. J. exp. Biol. med. Sci. 37, 523.CrossRefGoogle Scholar
Carlson, L. A. & Wadström, L. B. (1959). Clin. chim. acta, 4, 197.CrossRefGoogle Scholar
Eegriwe, E. (1937). Z. anal. Chem. 110, 22.CrossRefGoogle Scholar
Hehner, O. (1887). Analyst, 12, 65.CrossRefGoogle Scholar
MacFadyen, D. A. (1945). J. biol. Chem. 158, 107.CrossRefGoogle Scholar
Rhodes, D. N. (1959). Biochem. J. 71, 268.Google Scholar
Van Handel, E. & Zilversmit, D. B. (1957). J. Lab. clin. Med. 50, 152.Google Scholar