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A method of maintaining a reference flora of constant bacteriological composition

Published online by Cambridge University Press:  01 June 2009

B. Reiter ,
T. F. Fryer  and
M. Elisabeth Sharpe
B. Reiter
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading
T. F. Fryer
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading
M. Elisabeth Sharpe
Affiliation:
National Institute for Research in Dairying, Shinfield, Reading
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Summary

A method is described by which the microbial flora of a raw milk sample was reconstituted on different occasions. After segregation on selective media into groups consisting of lactic acid bacteria, Gram-negative rods including coli-aerogenes, staphylococci, streptococci, and microbacteria, and propagation by replica plate techniques on optimal media, suspensions of these groups of organisms were then added in approximately the original proportions to 40 gal batches of heated milk of low bacterial count. When this was repeated on 18 different occasions over a period of 11 weeks, the milk flora was found to be almost identical with the original one.

Type
Original Articles
Information
Journal of Dairy Research , Volume 32 , Issue 1 , February 1965 , pp. 89 - 96
Copyright
Copyright © Proprietors of Journal of Dairy Research 1965

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References

REFERENCES

American Public Health Association (1953). Standard Methods for the Examination of Dairy Products, 10th ed. New York: American Public Health Association.Google Scholar
Barnes, E. M. (1956). J. appl. Bact. 19, 193.Google Scholar
Chapman, G. H. (1945). J. Bact. 50, 201.CrossRefGoogle Scholar
de Man, J. C, Rogosa, M. & Sharpe, M. E. (1960). J. appl. Bact. 23, 130.CrossRefGoogle Scholar
Franklin, J. G. & Sharpe, M. E. (1963). J. Dairy Res. 30, 87.CrossRefGoogle Scholar
Lederberg, J. & Lederberg, E. M. (1952). J. Bact. 63, 399.CrossRefGoogle Scholar
Mabbitt, L. A., Chapman, H. R. & Sharpe, M. E. (1959). J. Dairy Res. 26, 105.CrossRefGoogle Scholar
Reiter, B., Fewins, B. G., Fryer, T. F. & Sharpe, M. E. (1964). J. Dairy Res. 31, 261.CrossRefGoogle Scholar
Roberts, C. F. (1959). J. gen. Microbiol. 20, 540.CrossRefGoogle Scholar
Rogosa, M., Mitchell, J. A. & Wiseman, R. F. (1951). J. dent. Res. 30, 682.Google Scholar
Weinberg, E. D. & Arthur, H. R. (1960). Lab. Pract. 9, 231.Google Scholar