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Metabolism of [U-14C]D-fructose by the isolated perfused udder

Published online by Cambridge University Press:  01 June 2009

R. Verbeke ,
Anne-Marie Massart-Leën  and
G. Peeters
R. Verbeke
Affiliation:
Physiological Department of the Veterinary College, University of Ghent, Belgium
Anne-Marie Massart-Leën
Affiliation:
Physiological Department of the Veterinary College, University of Ghent, Belgium
G. Peeters
Affiliation:
Physiological Department of the Veterinary College, University of Ghent, Belgium
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Summary

A lactating mammary gland of a sheep and a goat were perfused for several hours in the presence of [U-14C]D-fructose and received adequate quantities of acetate, glucose and amino acids.

In both experiments, there was a small incorporation of 14C in the expired CO2. Smaller radioactivities were measured in milk citric acid, lactose, casein and fat, the activities decreasing in that order. The specific activities of the amino acids from one casein hydrolysate were determined. The highest radioactivities were found in alanine and serine; methionine, glutamic acid and aspartic acid showed a smaller incorporation of 14C.

These results indicate that fructose is metabolized only to a very limited extent by the mammary gland. The relative distribution of 14C observed among the different substances isolated may be explained by a direct splitting of fructose into two C3-fragments, glycolysis and metabolism via the Krebs cycle.

Type
Original Articles
Information
Journal of Dairy Research , Volume 36 , Issue 2 , June 1969 , pp. 233 - 239
Copyright
Copyright © Proprietors of Journal of Dairy Research 1969

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References

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