Hostname: page-component-586b7cd67f-r5fsc Total loading time: 0 Render date: 2024-11-26T15:48:34.614Z Has data issue: false hasContentIssue false
  • English
  • Français

Lipoprotein lipase of goat mammary tissue: comparison of extraction methods

Published online by Cambridge University Press:  01 June 2009

Yves Chilliard
Yves Chilliard
Affiliation:
Animal Science Department Research Laboratory, INRA Institut National Agronomique Paris-Grignon, 16, rue Claude Bernard – 75231 Paris Cedex 05, France
Get access Rights & Permissions [Opens in a new window]

Summary

Mammary lipoprotein lipase of lactating goats was extracted by 3 methods: homogenization of tissue acetone-ether powders; aqueous homogenization of crude tissue using an Ultra-Turrax apparatus; aqueous homogenization of crude tissue using a Sorvall Omni-mixer microhomogenizer. Although there were differences between absolute values obtained by the 3 methods, each type of homogenate had a lipolytic activity with lipoprotein lipase characteristics (i.e. more than 90% inhibition by serum omission or NaCl addition). Furthermore, the 3 methods were highly correlated and presented similar variations with the stage of lactation, in parallel with long-chain fatty acid secretion into milk. Repeatability of the measure of homogenate lipolytic activities was about 8%, whereas day-to-day repeatability of enzyme extraction and assay was about 20% for each method.

Type
Original Articles
Information
Journal of Dairy Research , Volume 46 , Issue 4 , November 1979 , pp. 599 - 605
Copyright
Copyright © Proprietors of Journal of Dairy Research 1979

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Antonis, A. (1965). Journal of Lipid Research 6, 307.CrossRefGoogle Scholar
Askew, E. W. (1970). Enzymic aspects of fatty acid uptake and esterification by the bovine mammary gland. Ph.D. thesis. Michigan State Univ., East Lansing. USA.Google Scholar
Askew, E. W., Emery, R. S. & Thomas, J. W. (1970). Journal of Dairy Science 53, 1415.CrossRefGoogle Scholar
Barry, J. M., Bartley, W., Linzell, J. L. & Robinson, D. S. (1963). Biochemical Journal 89, 6.CrossRefGoogle Scholar
Bas, P. (1976). Influence du propionate de sodium et de la nicotinamide sur le métabolisme lipidique de la chèvre en lactation. Mémoire d'Ingénieur du Conservatoire National des Arts et Métiers, Paris.Google Scholar
Borensztajn, J., Otway, S. & Robinson, D. S. (1970). Journal of Lipid Research 11, 102.CrossRefGoogle Scholar
Brumby, P. E., Anderson, M., Tuckley, B., Storry, J. E. & Hibbitt, K. G. (1975). Biochemical Journal 146, 609.CrossRefGoogle Scholar
Chilliard, Y. & Fehr, P. M. (1976). Annales de Technologie agricole 25, 219.Google Scholar
Chilliard, Y., Dorléans, M. & Fehr, P. M. (1977). Annales de Biologie animale. Biochimie, Biophysique 17, 107.CrossRefGoogle Scholar
Chilliard, Y., Sauvant, D., Morand-Fehr, P. & Durand, G. (1978). Comptes rendus hebdomadaires des Séances de l'Académie des Sciences D 287, 871.Google Scholar
Deman, J. M. (1964). Journal of Dairy Science 47, 546.CrossRefGoogle Scholar
Emery, R. S. (1973). Journal of Dairy Science 56, 1187.CrossRefGoogle Scholar
Falconer, I. R. & Fiddler, T. J. (1970). Biochimica et Biophysica Acta 218, 508.CrossRefGoogle Scholar
Fielding, C. J. (1968). Biochimica et Biophysica Acta 159, 94.CrossRefGoogle Scholar
Fielding, C. J. & Fielding, P. E. (1976). Journal of Lipid Research 17, 248.CrossRefGoogle Scholar
Hamosh, M., Clary, T. R., Chernick, S. S. & Scow, R. O. (1970). Biochimica et Biophysica Acta 210, 473.CrossRefGoogle Scholar
Korn, E. D. (1955). Journal of Biological Chemistry 215, 1.CrossRefGoogle Scholar
Linzell, J. L. (1974). In Lactation, vol. I, p. 143 (Eds Larson, B. L. and Smith, V. R..) New York: Academic Press.Google Scholar
McBride, O. W. & Korn, E. D. (1963). Journal of Lipid Research 4, 17.CrossRefGoogle Scholar
Marx, G. D. & Caruolo, E. V. (1963). Journal of Dairy Science 46, 576.CrossRefGoogle Scholar
Mendelson, C. R., Zinder, O., Blanchette-Mackie, E. J., Chernick, S. S. & Scow, R. O. (1977). Journal of Dairy Science 60, 666.CrossRefGoogle Scholar
Okuda, H. & Fujii, S. (1973). Journal of Biochemistry 73, 1195.CrossRefGoogle Scholar
Plucinski, T. & Baldwin, R. L. (1976). Journal of Dairy Science 59, 157.CrossRefGoogle Scholar
Robinson, D. S. (1963a). Journal of Lipid Research 4, 21.CrossRefGoogle Scholar
Robinson, D. S. (1963b). Advances in Lipid Research 1, 133.CrossRefGoogle Scholar
Rogers, M. P., Barnett, D. & Robinson, D. S. (1976). Atherosclerosis 24, 551.CrossRefGoogle Scholar
Scow, R. O., Chernick, S. S. & Fleck, T. R. (1977). Biochimica et Biophysica Acta 487, 297.CrossRefGoogle Scholar
Shirley, J. E. (1973). Regulatory aspects of triglyceride uptake by bovine adipose and mammary tissue. Ph.D. Thesis, Michigan State University, East Lansing, USA.Google Scholar