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Lipase redistribution in cows' milk during induced lipolysis II. Activation by milk pH adjustment

Published online by Cambridge University Press:  01 June 2009

Philippe Cartier ,
Yves Chilliard  and
Josiane Bout
Philippe Cartier
Affiliation:
Laboratoire de la Lactation, INRA, Theix 63122 – Saint-Genès-Champanelle, France
Yves Chilliard
Affiliation:
Laboratoire de la Lactation, INRA, Theix 63122 – Saint-Genès-Champanelle, France
Josiane Bout
Affiliation:
Laboratoire de la Lactation, INRA, Theix 63122 – Saint-Genès-Champanelle, France
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Summary

Cold-stored milk lipolysis was enhanced from 5- to 50-fold when milk pH was adjusted to 7–8 with NaOH, while it was greatly decreased or stopped by an adjustment to pH 6–5·5 with citric acid. Small adjustments in pH (≤ 0·5 pH unit) also affected lipolysis, but the pH of native milk was not related to spontaneous lipolysis. The binding of lipoprotein lipase to cream was a pH-dependent process with an optimum near pH 7·0. Activity of the cream lipase on cold-stored milk fat continuously increased from pH 6·6 to pH 8·5. The activating effect of heparin on cold-stored milk lipolysis reached a maximum at pH 7·0–8·0 and a minimum in native milk, while addition of blood serum gave an opposite response.

Type
Original articles
Information
Journal of Dairy Research , Volume 56 , Issue 5 , November 1989 , pp. 711 - 718
Copyright
Copyright © Proprietors of Journal of Dairy Research 1989

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References

REFERENCES

Ahrné, L. & Björck, L. 1985 Lipolysis and the distribution of lipase activity in bovine milk in relation to stage of lactation and time of milking. Journal of Dairy Research 52 55–64CrossRefGoogle ScholarPubMed
Anderson, M. 1983 Milk lipase and off-flavour development. Journal of the Society of Dairy Technology 36 3–7CrossRefGoogle Scholar
Bachman, K. C. 1982 Effect of exogenous estradiol and progesterone upon lipase activity and spontaneous lipolysis in bovine milk. Journal of Dairy Science 65 907–914CrossRefGoogle ScholarPubMed
Bengtsson, G. & Olivecrona, T. 1982 On the pH dependency of lipoprotein lipase activity. Biochimica et Biophysica Acta 712 196–199CrossRefGoogle Scholar
Bengtsson, G. & Olivecrona, T. 1983 The effects of pH and salt on the lipid binding and enzyme activity of lipoprotein lipase. Biochimica et Biophysica Acta 751 254–259CrossRefGoogle ScholarPubMed
Cartier, P. & Chilliard, Y. 1989 Lipase redistribution in cows' milk during induced lipolysis. I. Activation by agitation, temperature change, blood serum and heparin. Journal of Dairy Research 56 699–709CrossRefGoogle ScholarPubMed
Chazal, M. P. & Chilliard, Y. 1986 Effect of stage of lactation, stage of pregnancy, milk yield and herd management on seasonal variation in spontaneous lipolysis in bovine milk. Journal of Dairy Research 53 529–538CrossRefGoogle ScholarPubMed
Chilliard, Y. 1982 [Physiological variations in lipase activities and spontaneous lipolysis in bovine, caprine and human milk: a review.] Lait 62 1–31: 126–154CrossRefGoogle Scholar
Chilliard, Y. & Lamberet, G. 1984 [Milk lipolysis: different types, mechanisms, variation factors and practical significance.] Lait 64 544–578CrossRefGoogle Scholar
Deeth, H. C. & Fitz-Gerald, C. H. 1975 Factors governing the susceptibility of milk to spontaneous lipolysis. International Dairy Federation Annual Bulletin Document No. 86 24–34Google Scholar
Downey, W. K. 1975 Identity of the major lipolytic enzyme activity of bovine milk in relation to spontaneous and induced lipolysis. International Dairy Federation Annual Bulletin Document No. 86 80–89Google Scholar
Driessen, F. M. & Stadhouders, J. 1974 A study of spontaneous rancidity. Netherlands Milk and Dairy Journal 28 130–145Google Scholar
Egelrud, T. & Olivecrona, T. 1973 Purified bovine milk (lipoprotein) lipase: activity against lipid substrates in the absence of exogenous serum factors. Biochimica et Biophysica Acta 306 115–127CrossRefGoogle ScholarPubMed
Jenness, R., Shipe, W. F. & Sherbon, J. W. 1974 Physical properties of milk. In Fundamentals of Dairy Chemistry 2nd edn pp. 402–441 (Eds Webb, B. H., Johnson, A. H. and Alford, J. A.). Westport, CT: Avi Publishing Co. Inc.Google Scholar
Murphy, J. J., Connolly, J. F. & Headon, D. R. 1979 A study of factors associated with free fatty acid development in milk. Irish Journal of Food Science and Technology 3 131–149Google Scholar
Olivecrona, T. 1980 Biochemical aspects of lipolysis in bovine milk. International Dairy Federation Bulletin Document No. 118 19–25Google Scholar
Olivecrona, T. & Bengtsson, G. 1984 Lipases in milk. In Lipases pp. 205–261 (Eds Borgström, B. and Brockman, H. L.). Amsterdam: ElsevierGoogle Scholar
Quinn, D., Shirai, K. & Jackson, R. L. 1982 Lipoprotein lipase: mechanism of action and role in lipoprotein metabolism. Progress in Lipid Research 22 35–78CrossRefGoogle Scholar
Rapp, D. & Olivecrona, T. 1978 Kinetics of milk lipoprotein lipase. Studies with tributyrin. European Journal of Biochemistry 91 379–385CrossRefGoogle ScholarPubMed
Sundheim, G. & Bengtsson-Olivecrona, G. 1985 Lipolysis in milk induced by cooling or by heparin: comparisons of amount of lipoprotein lipase in the cream fraction and degree of lipolysis. Journal of Dairy Science 68 589–593CrossRefGoogle Scholar
Sundheim, G. & Bengtsson-Olivecrona, G. 1986 Iodine-125-labeled lipoprotein lipase as a tool to detect and study spontaneous lipolysis in bovine milk. Journal of Dairy Science 69 1776–1783CrossRefGoogle ScholarPubMed