Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-19T22:56:00.327Z Has data issue: false hasContentIssue false
  • English
  • Français

Leucocyte expression of genes implicated in the plasminogen activation cascade is modulated by yoghurt peptides

Published online by Cambridge University Press:  07 September 2016

Georgios Theodorou  and
Ioannis Politis
Georgios Theodorou*
Affiliation:
Department of Animal Science and Aquaculture, Agricultural University of Athens, Athens, Greece
Ioannis Politis
Affiliation:
Department of Animal Science and Aquaculture, Agricultural University of Athens, Athens, Greece
*
*For correspondence; e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The urokinase-plasminogen activator (u-PA), its receptor (u-PAR) and the inhibitors of u-PA (PAI-1 and PAI-2) provide a multi-molecular system in leucocytes that exerts pleiotropic functions influencing the development of inflammatory and immune responses. The objective of the present study was to examine the ability of water soluble extracts (WSE) obtained from traditional Greek yoghurt made from bovine or ovine milk to modulate the expression of u-PA, u-PAR, PAI-1 and PAI-2 in ovine monocytes and neutrophils. WSE were obtained from 8 commercial traditional type Greek yoghurts made from ovine or bovine milk. WSE upregulated the expression of all 4 u-PA related genes in monocytes but the upregulation was much higher in the PAI-1 (10-fold) than in u-PA and u-PAR (3–4 fold) thus, shifting the system towards inhibition. In line with this observation, WSE reduced total and membrane-bound u-PA activity in monocytes. In neutrophils, WSE caused small (50–60%) but significant (P < 0·05) reductions in expression of u-PAR and PAI-2 but had no effect on expression of u-PA, PAI-1 and on total cell-associated and membrane-bound u-PA activity. WSE from yoghurts made from bovine or ovine milk were essentially equally effective in affecting the u-PA system except for the u-PAR gene in ovine neutrophils that was affected (reduced) by the ovine and not the bovine WSE. In conclusion, peptides present in WSE modulated the expression of u-PA related genes but the effect was much more prominent in monocytes than in neutrophils.

Keywords

Yoghurtplasminogen activation systemneutrophilsmonocytes
Type
Research Article
Information
Journal of Dairy Research , Volume 83 , Issue 3 , August 2016 , pp. 360 - 365
Copyright
Copyright © Proprietors of Journal of Dairy Research 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

AOAC 1990 Official Methods of Analysis. Arlington, VA: Association of Official Analytical Chemists, Inc Google Scholar
Baldi, A, Politis, I, Pecorini, C, Fusi, E, Chronopoulou, R & Dell'Orto, V 2005 Biological effects of milk proteins and their peptides with emphasis on those related to the gastrointestinal ecosystem. Journal of Dairy Research 72 66–72 CrossRefGoogle Scholar
Dale, MA, Ruhlman, MK & Baxter, BT 2015 Inflammatory cell phenotypes in AAAs: their role and potential as targets for therapy. Arteriosclerosis, Thrombosis, and Vascular Biology 35 1746–1755 CrossRefGoogle ScholarPubMed
Degryse, B, Sier, CF, Resnati, M, Conese, M & Blasi, F 2001 PAI-1 inhibits urokinase-induced chemotaxis by internalizing the urokinase receptor. FEBS letters 505 249–254 CrossRefGoogle ScholarPubMed
Del Rosso, M, Fibbi, G, Pucci, M, Margheri, F & Serrati, S 2008 The plasminogen activation system in inflammation. Frontiers in Bioscience 13 4667–4686 CrossRefGoogle ScholarPubMed
Del Rosso, M, Margheri, F, Serrati, S, Chilla, A, Laurenzana, A & Fibbi, G 2011 The urokinase receptor system, a key regulator at the intersection between inflammation, immunity, and coagulation. Current Pharmaceutical Design 17 1924–1943 CrossRefGoogle ScholarPubMed
Hellemans, J, Mortier, G, De Paepe, A, Speleman, F & Vandesompele, J 2007 qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data. Genome Biology 8 R19 CrossRefGoogle ScholarPubMed
Korhonen, H & Pihlanto, A 2007 Technological options for the production of health-promoting proteins and peptides derived from milk and colostrum. Current Pharmaceutical Design 13 829–843 CrossRefGoogle ScholarPubMed
Kucharewicz, I, Kowal, K, Buczko, W & Bodzenta-Lukaszyk, A 2003 The plasmin system in airway remodeling. Thrombosis Research 112 1–7 CrossRefGoogle ScholarPubMed
Kuchroo, CN & Fox, PF 1982 Soluble nitrogen in Cheddar cheese: comparison of extraction procedures. Milchwissenschaft 37 331–335 Google Scholar
Law, J & Haandrikman, A 1997 Proteolytic enzymes of lactic acid bacteria. International Dairy Journal 7 1–11 CrossRefGoogle Scholar
Marshall, OJ 2004 PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR. Bioinformatics 20 2471–2472 CrossRefGoogle ScholarPubMed
Pfaffl, MW 2001 A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Research 29 e45 CrossRefGoogle ScholarPubMed
Plesner, T, Behrendt, N & Ploug, M 1997 Structure, function and expression on blood and bone marrow cells of the urokinase-type plasminogen activator receptor, uPAR. Stem Cells (Dayton, Ohio) 15 398–408 CrossRefGoogle ScholarPubMed
Politis, I 1996 Plasminogen activator system: implications for mammary cell growth and involution. Journal of Dairy Science 79 1097–1107 CrossRefGoogle ScholarPubMed
Politis, I & Chronopoulou, R 2008 Milk peptides and immune response in the neonate. Advances in Experimental Medicine and Biology 606 253–269 CrossRefGoogle ScholarPubMed
Politis, I, Hidiroglou, N, White, JH, Gilmore, JA, Williams, SN, Scherf, H & Frigg, M 1996 Effects of vitamin E on mammary and blood leukocyte function, with emphasis on chemotaxis, in periparturient dairy cows. American Journal of Veterinary Research 57 468–471 CrossRefGoogle ScholarPubMed
Politis, I, Hidiroglou, N, Cheli, F & Baldi, A 2001 Effects of vitamin E on urokinase-plasminogen activator receptor expression by bovine neutrophils. American Journal of Veterinary Research 62 1934–1938 CrossRefGoogle ScholarPubMed
Politis, I, Zavizjon, B, Cheli, F & Baldi, A 2002 Expression of urokinase plasminogen activator receptor in resting and activated bovine neutrophils. Journal of Dairy Research 69 195–204 CrossRefGoogle ScholarPubMed
Politis, I, Theodorou, G, Lampidonis, AD, Chronopoulou, R & Baldi, A 2012 Soya protein hydrolysates modify the expression of various pro-inflammatory genes induced by fatty acids in ovine phagocytes. British Journal of Nutrition 108 1246–1255 CrossRefGoogle ScholarPubMed
Reichel, CA, Kanse, SM & Krombach, F 2012 At the interface of fibrinolysis and inflammation: the role of urokinase-type plasminogen activator in the leukocyte extravasation cascade. Trends in Cardiovascular Medicine 22 192–196 CrossRefGoogle ScholarPubMed
Sitrin, RG, Pan, PM, Blackwood, RA, Huang, J & Petty, HR 2001 Cutting edge: evidence for a signaling partnership between urokinase receptors (CD87) and L-selectin (CD62L) in human polymorphonuclear neutrophils. Journal of Immunology 166 4822–4825 CrossRefGoogle Scholar
Theodorou, G, Daskalopoulou, M, Chronopoulou, R, Baldi, A, Dell'orto, V & Politis, I 2010 Acute mastitis induces upregulation of expression of plasminogen activator-related genes by blood monocytes and neutrophils in dairy ewes. Veterinary Immunology and Immunopathology 138 124–128 CrossRefGoogle ScholarPubMed