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Isoelectric focusing of milk-clotting enzymes

Published online by Cambridge University Press:  01 June 2009

P. G. Righetti ,
Bruna M. Molinari  and
G. Molinari
P. G. Righetti
Affiliation:
Department of Biochemistry, University of Milano, Via Celoria 2, Milano 20133, Italy
Bruna M. Molinari
Affiliation:
La Biotecnica, Via Torre d'Augusto 51, Trento 38100, Italy
G. Molinari
Affiliation:
La Biotecnica, Via Torre d'Augusto 51, Trento 38100, Italy
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Summary

Isoelectric focusing in thin-layer polyacrylamide gel has been applied successfully to the characterization and identification of calf rennet and its substitutes. The use of acidic pH gradients (pH range 2·5–6) allows the identification of calf and microbial rennets and bovine and pig pepsins.

A new, very rapid and sensitive zymogram technique has been developed by overlaying the gel with a highly porous, transparent cellulose acetate film impregnated with casein.

Type
Original Articles
Information
Journal of Dairy Research , Volume 44 , Issue 1 , February 1977 , pp. 69 - 72
Copyright
Copyright © Proprietors of Journal of Dairy Research 1977

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References

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