Hostname: page-component-78c5997874-lj6df Total loading time: 0 Render date: 2024-11-06T10:52:23.214Z Has data issue: false hasContentIssue false
  • English
  • Français

Immunomodulatory effects of dietary whey proteins in mice

Published online by Cambridge University Press:  01 June 2009

Chun W. Wong  and
Dennis L. Watson
Chun W. Wong
Affiliation:
CSIRO Division of Animal Health, Armidale, NSW 2350, Australia
Dennis L. Watson
Affiliation:
CSIRO Division of Animal Health, Armidale, NSW 2350, Australia
Get access Rights & Permissions [Opens in a new window]

Summary

Studies on the immunomodulatory properties of dietary whey proteins in mice are reported. Ingestion of bovine milk whey proteins, either as a supplement in an adequately balanced commercial diet or as the only protein source in a balanced diet, consistently enhanced secondary humoral antibody responses following systemic immunization with ovalbumin, when compared with other protein sources such as soyabean protein isolate and ovine colostral whey proteins. After 5–8 weeks of feeding, dietary milk whey proteins enhanced cell-mediated immune responses as revealed by footpad delayed-type hypersensitivity responses, and concanavalin A-induced spleen cell proliferative responses. To monitor nutritional effects of milk whey proteins, live weight, leucocyte counts and clinical changes of diet-fed mice were examined. The present results confirm other previous results that dietary bovine milk whey proteins have immunoenhancing properties in mice and these properties are unlikely to be related solely to the nutritional effects.

Type
Original Articles
Information
Journal of Dairy Research , Volume 62 , Issue 2 , May 1995 , pp. 359 - 368
Copyright
Copyright © Proprietors of Journal of Dairy Research 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Barta, O., Barta, V. D., Crisman, M. V. & Akers, R. M. 1991 Inhibition of lymphocyte blastogenesis by whey. American Journal of Veterinary Research 52 247–253CrossRefGoogle ScholarPubMed
Belokrylov, G. A., Popova, O. Ya., Molchanova, I. V., Sorochinskaya, E. I. & Anokhina, V. V. 1992 Peptides and their constituent amino acids influence the immune response and phagocytosis in different ways. International Journal of Immunopharmacology 14 1285–1292CrossRefGoogle ScholarPubMed
Bounous, G., Baruchel, S., Falutz, J. & Gold, P. 1993 Whey proteins as a food supplement in HIV-seropositive individuals. Clinical and Investigative Medicine 16 204–209Google ScholarPubMed
Bounous, G., Batist, G. & Gold, P. 1989 Immunoenhancing property of dietary whey protein in mice: role of glutathione. Clinical and Investigative Medicine 12 154–161Google ScholarPubMed
Bounous, G. & Gold, P. 1991 The biological activity of undenatured dietary whey proteins: role of glutathione. Clinical and Investigative Medicine 14 296–309Google ScholarPubMed
Bounous, G. & Kongshavn, P. A. L. 1982 Influence of dietary proteins on the immune system of mice. Journal of Nutrition 112 1747–1755CrossRefGoogle ScholarPubMed
Bounous, G. & Kongshavn, P. A. L. 1985 Differential effect of dietary protein type on the B-cell and T-cell immune responses in mice. Journal of Nutrition 115 1403–1408CrossRefGoogle ScholarPubMed
Bounous, G., Kongshavn, P. A. L. & Gold, P. 1988 a The immunoenhancing property of dietary whey protein concentrate. Clinical and Investigative Medicine 11 271–278Google ScholarPubMed
Bounous, G., Papenburg, R., Kongshavn, P. A. L., Gold, P. & Fleiszer, D. 1988 b Dietary whey protein inhibits the development of dimethylhydrazine induced malignancy. Clinical and Investigative Medicine 11 213–217Google ScholarPubMed
Bounous, G., Shenouda, N., Kongshavn, P. A. L. & Osmond, D. G. 1985 Mechanism of altered B-cell response induced by changes in dietary protein type in mice. Journal of Nutrition 115 1409–1417CrossRefGoogle ScholarPubMed
Chaplin, D. D. & Wedner, H. J. 1978 Inhibition of lectin-induced lymphocyte activation by diamide and other sulfhydryl reagents. Cellular Immunology 36 303–311CrossRefGoogle ScholarPubMed
Eigel, W. N., Butler, J. E., Ernstrom, C. A., Farrell, H. M., Harwalkar, V. R., Jenness, R. & Whitney, R. Mcl. 1984 Nomenclature of proteins of cow's milk: fifth revision. Journal of Dairy Science 67 1599–1631CrossRefGoogle Scholar
Fidelus, R. K. & Tsan, M. F. 1987 Glutathione and lymphocyte activation: a function of ageing and autoimmune disease. Immunology 61 503–508Google Scholar
Fischman, C. M., Udey, M. C., Kurtz, M. & Wedner, H. J. 1981 Inhibition of lectin-induced lymphocyte activation by 2-cyclohexene-l-one: decreased intracellular glutathione inhibits an early event in the activation sequence. Journal of Immunology 127 2257–2262CrossRefGoogle ScholarPubMed
Juto, P. 1985 Human milk stimulates B cell function. Archives of Disease in Childhood 60 610–613CrossRefGoogle ScholarPubMed
Liew, F. Y. 1977 Regulation of delayed-type hypersensitivity. I. T suppressor cells for delayed-type hypersensitivity to sheep erythrocytes in mice. European Journal of Immunology 7 714–718CrossRefGoogle Scholar
Mciktosh, G. H. 1993 Dairy proteins: their influence on colon cancer risk. Dairy Research and Development Corporation Nutrition Workshop Biographical Data (Abstract). Melbourne, 18–19 02. 14–15Google Scholar
Meister, A. & Anderson, M. E. 1983 Glutathione. Annual Review of Biochemistry 52 711–760CrossRefGoogle ScholarPubMed
Mincheva-Nilsson, L., Hammarström, M. L., Juto, P. & Hammarström, S. 1990 Human milk contains proteins that stimulate and suppress T lymphocyte proliferation. Clinical and Experimental Immunology 79 463–469CrossRefGoogle ScholarPubMed
Noelle, R. J. & Lawrence, D. A. 1981 Determination of glutathione in lymphocytes and possible association of redox state and proliferative capacity of lymphocytes. Biochemical Journal 198 571–579CrossRefGoogle ScholarPubMed
Ogra, S. S. & Ogra, P. L. 1978 Immunologic aspects of human colostrum and milk. II. Characteristics of lymphocyte reactivity and distribution of E-rosette forming cells at different times after onset of lactation. Journal of Pediatrics 92 550–555CrossRefGoogle ScholarPubMed
Schalm, O. W., Jain, N. C. & Carroll, E. J. (Eds) 1986 Veterinary Hematology, 4th edn. Philadelphia, PA: Lea & FebigerGoogle Scholar
Stoeok, M., Ruegg, C., Miescher, S., Carrel, S., Cox, D., Von Fliedner, V. & Alkan, S. 1989 Comparison of the immunosuppressive properties of milk growth factor and transforming growth factors β1 and β2. Journal of Immunology 143 3258–3265CrossRefGoogle Scholar
Watson, D. L. 1990 Immunological activity of factors in colostrum and milk. In Joint Convention Papers, Gold Coast, QLD, 6th–10thMay, pp. 81–83 (Eds Halais, C., Deeth, H., Fedrick, I., Jehne, C., Leith, G., Macfarlane, J. and Paroz, P.). The QLD Branch of The Australian and New Zealand Institutes of Food Science and Technology LtdGoogle Scholar
Watson, D. L., Francis, G. L. & Ballard, F. J. 1992 Factors in ruminant colostrum that influence cell growth and murine IgE antibody responses. Journal of Dairy Research 59 369–380CrossRefGoogle ScholarPubMed