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Immunological identification of milk-clotting enzymes

Published online by Cambridge University Press:  01 June 2009

Jean-Claude Collin ,
Graciela Muset de Retta  and
Patrice Martin
Jean-Claude Collin
Affiliation:
Institut National de la Recherche Agronomique, CNRZ
Graciela Muset de Retta
Affiliation:
Institut National de la Recherche Agronomique, CNRZ
Patrice Martin
Affiliation:
Institut National de la Recherche Agronomique, CNRZ
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Summary

The 6 most widely used milk-clotting enzymes, i.e. chymosin, bovine and porcine pepsins and proteinases from Mucor miehei, M. pusillus and Endothia parasitica, have been purified and used to prepare rabbit antisera against each of them. The antisera were adsorbed with appropriate cross-linked antigens to remove non-specific antibodies. Monospecific antisera thus obtained were used to identify enzymes eventually added to calf or bovine rennets, using the double radial immunodiffusion technique. The threshold of sensitivity was c. 1%, expressed as clotting activity. This qualitative method complements the quantitative procedure recently proposed for determining chymosin and bovine pepsin A in commercial extracts of bovine veils.

Type
Original Articles
Information
Journal of Dairy Research , Volume 49 , Issue 2 , May 1982 , pp. 221 - 230
Copyright
Copyright © Proprietors of Journal of Dairy Research 1982

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References

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