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Identification of fibroblast growth factors in bovine cheese whey

Published online by Cambridge University Press:  01 June 2009

Mary-Louise Rogers ,
David A. Belford ,
Geoffrey L. Francis  and
F. John Ballard
Mary-Louise Rogers
Affiliation:
Cooperative Research Centre for Tissue Growth and Repair, PO Box 10065, Gouger Street, Adelaide, SA 5000, Australia
David A. Belford
Affiliation:
Cooperative Research Centre for Tissue Growth and Repair, PO Box 10065, Gouger Street, Adelaide, SA 5000, Australia
Geoffrey L. Francis
Affiliation:
Cooperative Research Centre for Tissue Growth and Repair, PO Box 10065, Gouger Street, Adelaide, SA 5000, Australia
F. John Ballard
Affiliation:
Cooperative Research Centre for Tissue Growth and Repair, PO Box 10065, Gouger Street, Adelaide, SA 5000, Australia
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Summary

Acidic and basic fibroblast growth factors (FGF) were identified in bovine cheese whey after partial purification using a two step procedure. Cation-exchange chromatography produced a mitogen-rich extract which was loaded on to a heparin-sepharose column and eluted stepwise with 0·8, 1·2 and 2·0 M-NH4HC03. Mitogenic activity was found in all three fractions by cell growth assays using Balb/c-3T3 fibroblasts. Immunoblotting identified acidic FGF in the 1·2 M-eluate and basic FGF in the 2·0 M-eluate, but neither acidic nor basic FGF was detected in the 0·8 M-fraction. Quantitative radioreceptor assays indicated 5·8 ng of acidic FGF-like activity and 19·8 ng of basic FGF-like activity per 1 whey in the appropriate eluates. This study represents the first direct demonstration of FGF in milk.

Type
Original Articles
Information
Journal of Dairy Research , Volume 62 , Issue 3 , August 1995 , pp. 501 - 507
Copyright
Copyright © Proprietors of Journal of Dairy Research 1995

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