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Heat stability of milk: influence of modification of lysine and arginine on the heat stability-pH profile

Published online by Cambridge University Press:  01 June 2009

Samweul I. Shalabi  and
Patrick F. Fox
Samweul I. Shalabi
Affiliation:
Department of Dairy and Food Chemistry, University College, Cork, Irish Republic
Patrick F. Fox
Affiliation:
Department of Dairy and Food Chemistry, University College, Cork, Irish Republic
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Summary

Several dicarbonyl compounds (glyoxal, substituted glyoxals, diacetyl and 1, 2-cyclohexadione) had a marked stabilizing effect on the heat stability of milk, especially in the presence of urea. These reagents are believed to modify arginine more or less specifically suggesting an important role for arginine residues in heat stability. In contrast, modification of lysine residiles with dansyl chloride, acetic anhydride or cyanoborohydride had little effect on maximum heat stability although it did alter the HCT-pH profile. Since diacetyl is a natural constituent of fermented milks and cheese, it may be acceptable as an additive to increase the heat stability of ṃilk.

Type
Original Articles
Information
Journal of Dairy Research , Volume 49 , Issue 4 , November 1982 , pp. 607 - 617
Copyright
Copyright © Proprietors of Journal of Dairy Research 1982

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References

REFERENCES

Davies, D. T. & White, J. C. D. 1966 The stability of milk protein to heat. I. Subjective measurement of heat stability of milk. Journal of Dairy Research 33 67–81CrossRefGoogle Scholar
Fox, P. F. & Hearn, C. M. 1978 Heat stability of milk: influence of denaturable proteins and detergents on pH sensitivity. Journal of Dairy Research 45 159–172CrossRefGoogle Scholar
Fox, P. F., Nash, B. M., Horan, T. J., O'Brien, J. & Morrissey, P. A. 1980 Effect of selected amides on heat-induced changes in milk. Journal of Dairy Research 47 211–219CrossRefGoogle Scholar
Holt, C., Muir, D. D. & Swebtsur, A. W. M. 1978 The heat stability of milk and concentrated milk containing added aldehydes and sugars. Journal of Dairy Research 45 47–52CrossRefGoogle Scholar
Itano, H. A. & Gottlieb, A. J. 1963 Blocking of tryptic cleavage of arginyl bonds by the chemical modification of the guanido group with benzil. Biochemical and Biophysical Research Communications 12 405–408CrossRefGoogle ScholarPubMed
Keen, A. R. & Walker, N. J. 1974 Diacetyl, acetoin, 2, 3-butyleneglycol, 2-butanone and 2-butanol concentrations in ripening Cheddar cheese. Journal of Dairy Research 41 65–71CrossRefGoogle Scholar
Kosikowski, F. V. 1944 The effect of high temperature and various chemical agents upon the heat stability, browning and apparent changes in lactose concentration of whole and evaporated milk. Ph.D. Thesis, Cornell University, Ithaca, N.Y.Google Scholar
Kudo, S. 1980 Influence of lactose and urea on the heat stability of artificial milk systems. New Zealand Journal of Dairy Science & Technology 15 197–200Google Scholar
Kurtz, A. C. 1938 A simple synthesis of dl-citrulline. Journal of Biological Chemistry 122 477–484CrossRefGoogle Scholar
Liu, W. H., Feinstein, G., Osuga, D. T., Haynes, R. & Feeney, R. E. 1968 Modification of arginines in trypsin inhibitors by 1, 2-cyclohexanedione. Biochemistry 7 2886–2892CrossRefGoogle ScholarPubMed
Means, G. E. & Feeney, R. E. 1971 Chemical modification of proteins. San Francisco, Calif.: Holden-Day.Google Scholar
Nelson, V. 1954 Effects of formaldehyde and copper salts on the heat stability of evaporated milk. Journal of Dairy Science 37 825–829CrossRefGoogle Scholar
Patthy, L. & Smith, E. L. 1975 Reversible modification of arginine residues. Application to sequence studies by restriction of tryptic hydrolysis to lysine residues. Journal of Biological Chemistry 250 557–564CrossRefGoogle ScholarPubMed
Pyne, G. T. & Mchenry, K. A. 1955 The heat coagulation of milk. Journal of Dairy Research 22 60–68CrossRefGoogle Scholar
Shalabi, S. I. & Fox, P. F. 1982 a Heat stability of milk: synergic action of urea and carbonyl compounds. Journal of Dairy Research 49 197–207CrossRefGoogle Scholar
Shalabi, S. I. & Fox, P. F. 1982b Heat stability of milk: influence of cationic detergents on pH sensitivity. Journal of Dairy Research 49 597–605CrossRefGoogle ScholarPubMed
Stark, G. R., Stein, W. H. & Moore, S. 1960 Reactions of the cyanate present in aqueous urea with amino acids and proteins. Journal of Biological Chemistry 235 3177–3181CrossRefGoogle Scholar
Stevens, C. M. & Ellman, P. B. 1950 Non-utilization of α-amino-ε-ureido-n-caproic acid, piperidine 2- carboxylic acid and α-aminoadipic acid for growth in rats on a lysine-deficient diet. Journal of Biological Chemistry 182 75–79CrossRefGoogle Scholar
Sweetsur, A. W. M. & Muir, D. D. 1981 Role of cyanate ions in the urea-induced stabilization of the caseinate complex in skim-milk. Journal of Dairy Research 48 163–166CrossRefGoogle Scholar
Szent-GyöRgyi, A., EgyüD, L. G. & Maclaughlin, J. A. 1967 Keto-aldehydes and cell division. Science 155 539–541CrossRefGoogle ScholarPubMed
Takahashi, K. 1977 a The reactions of phenylglyoxal and related reagents with amino acids. Journal of Biochemistry 81 395–402CrossRefGoogle ScholarPubMed
Takahashi, K. 1977 b Further study on the reactions of phenylglyoxal and related reagents with proteins. Journal of Biochemistry 81 403–414CrossRefGoogle Scholar