Hostname: page-component-586b7cd67f-t8hqh Total loading time: 0 Render date: 2024-11-26T19:08:53.609Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic polymorphism of STAT5A protein: relationships with production traits and milk composition in Italian Brown cattle

Published online by Cambridge University Press:  29 July 2009

Maria Selvaggi ,
Cataldo Dario ,
Giovanni Normanno ,
Gaetano V Celano  and
Marco Dario
Maria Selvaggi
Affiliation:
Department of Animal Health and Welfare, University of Study of Bari, strada prov. le per Casamassima Km 3–70010 Valenzano (Ba)Italy
Cataldo Dario*
Affiliation:
Department of Animal Health and Welfare, University of Study of Bari, strada prov. le per Casamassima Km 3–70010 Valenzano (Ba)Italy
Giovanni Normanno
Affiliation:
Department of Animal Health and Welfare, University of Study of Bari, strada prov. le per Casamassima Km 3–70010 Valenzano (Ba)Italy
Gaetano V Celano
Affiliation:
Department of Animal Health and Welfare, University of Study of Bari, strada prov. le per Casamassima Km 3–70010 Valenzano (Ba)Italy
Marco Dario
Affiliation:
Department of Animal Health and Welfare, University of Study of Bari, strada prov. le per Casamassima Km 3–70010 Valenzano (Ba)Italy
*
*For correspondence; e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

STATs are a group of transcription factors that mediate actions of a variety of peptide hormones and cytokines within target cells (for example, prolactin and growth hormone). Therefore, STAT5A gene is a candidate marker for quantitative traits in farm animals with respect to milk production traits. In this study the STAT5A/AvaI polymorphism was investigated with PCR-RFLP in a sample of 233 Italian Brown cattle. This polymorphism is localized in the coding region of the bovine STAT5A gene. It is a substitution C→T at position 6853 within exon 7. All three possible genotypes for the C/T polymorphism were identified. The overall frequencies of alleles C and T were 0·83 and 0·17 respectively; the Hardy-Weinberg equilibrium was verified. In order to study the relationship between STAT5A/AvaI polymorphism and milk performance traits, the data for a 305-d milk production that included milk yield, protein and fat yield, fat and protein percentage were used. Significant differences between the two genotypes were found in yields of milk, fat and protein and protein percentage (P<0·01). CC cows produced more milk than CT (5418·68 v. 5149·54 kg). Protein content was higher in milk from CC compared with CT genotypes (3·40 v. 3·21%). No significant difference was found in fat content. Owing to the low number of TT cows in the studied population, this genotype was not included in the statistical analysis; in fact the number of TT cows was not enough to provide an accurate statistical analysis. Although more studies are needed to better clarify the role of this SNP on production traits, STAT5A/AvaI polymorphism appears to be a promising indirect marker to improve milk production traits in cattle.

Keywords

STAT5Agene polymorphismItalian Brown cattlemilk production traits
Type
Research Article
Information
Journal of Dairy Research , Volume 76 , Issue 4 , November 2009 , pp. 441 - 445
Copyright
Copyright © Proprietors of Journal of Dairy Research 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Antoniou, E, Hirts, BJ, Grosz, M & Skidmorec, J 1999 A single strand conformation polymorphism in the bovine gene STAT5A. Animal Genetics 30 225–244Google Scholar
Argetsinger, LS & Carter-Su, C 1996 Growth hormone signalling mechanisms: involvement of the tyrosine kinase JAK2. Hormone Research 45 22–24CrossRefGoogle ScholarPubMed
Bennewitz, J, Reinsch, N, Grohs, C, Leveziel, H, Malafosse, A, Thomsen, H, Xu, N, Looft, C, Kuhn, C, Brockmann, GA, Schwerin, M, Weimann, C, Hiendleder, S, Erhardt, G, Medjugorac, I, Russ, I, Forster, M, Brenig, B, Reinhardt, F, Reents, R, Averdunk, G, Blumel, J, Boichard, D & Kalm, E 2003 Combined analysis of data from two granddaughter designs: A simple strategy for QTL confirmation and increasing experimental power in dairy cattle. Genetics Selection Evolution 35 318–338Google Scholar
Boichard, D, Grohs, C, Bourgeois, F, Cerqueira, F, Faugeras, R, Neau, A, Rupp, R, Amigues, Y, Boscher, MY & Leveziel, H 2003 Detection of genes influencing economic traits in three French dairy cattle breeds. Genetics Selection Evolution 35 77–101Google Scholar
Boucheron, C, Dumon, S, Santos, SC, Moriggl, R, Hennighausen, L, Gisselbrecht, S & Gouilleux, F 1998 A single amino acid in the DNA binding regions of STAT5A and STAT5B confers distinct DNA binding specificities. Journal of Biological Chemistry 273 33936–33941CrossRefGoogle ScholarPubMed
Brym, P, Kamiński, S & Ruść, A 2004 New SSCP polymorphism within bovine STAT5A gene and its associations with milk performance traits in Black-and-White and Jersey cattle. Journal of Applied Genetics 45 445–452Google Scholar
Dario, C, Selvaggi, M, Carnicella, D & Bufano, G 2009 STAT5A/AvaI polymorphism in Podolica bulls and its effect on growth performances traits. Livestock Science 123 83–87Google Scholar
JrDarnell, JE, Kerr, IM & Stark, GR 1994 JAK-STAT pathways and transcriptional activation in response to IFNs and other extracellular signalling proteins. Science 264 1415–1421Google Scholar
Falconer, DS & Mackay, TFC 1996 Introduction to Quantitative Genetics. 4th Edn. Cheltenham, UK: Essex, UK: Longman Group LtdGoogle Scholar
Flisikowski, K, Szymanowska, M & Zwierzchowski, L 2003a The DNA binding capacity of genetic variants of the bovine STAT5A transcription factor. Cellular and Molecular Biology Letters 8 831–840Google ScholarPubMed
Flisikowski, K, Strzałkowska, N, Słoniewski, K, Krzyżewki, J & Zwierzchowski, L 2004 Association of a sequence nucleotide polymorphism in exon 16 of the STAT5A gene with milk production traits in Polish Black-and-White (Polish Friesian) cows. Animal Science Papers and Reports 22 515–522Google Scholar
Flisikowski, K & Zwierzchowski, L 2002 Single-strand conformation polymorphism within exon 7 of the bovine STAT5A gene. Animal Science Papers and Reports 20 133–137Google Scholar
Flisikowski, K & Zwierzchowski, L 2003 Polymerase chain reaction-heteroduplex (PCR-HD) polymorphism within the bovine STAT5A gene. Journal of Applied Genetics 44 185–189Google Scholar
Flisikowski, K, Oprzdek, J, Dymnicki, E & Zwierzchowski, L 2003b New polymorphism in bovine STAT5A gene and its association with meat production traits in beef cattle. Animal Science Papers and Reports 21 147–157Google Scholar
Goldammer, T, Meyer, L, Seyfert, H, Brunner, RM & Schwerin, M 1997 STAT5A encoding gene maps to chromosome 19 in cattle and goat and chromosome 11 in sheep. Mammalian Genome 8 705–706Google Scholar
Hou, J, Schindler, U, Henzel, WJ, Wong, SC & McKnight, SL 1995 Identification and purification of human STAT proteins activated in response to interleukin-2. Immunity 2 321–329Google Scholar
Kazansky, AV, Raught, B, Lindsey, SM, Wang, YF & Rosen, JM 1995 Regulation of mammary gland factor/STAT5A during mammary gland development. Molecular Endocrinology 9 1598–1609Google ScholarPubMed
Khatib, H, Monson, RL, Schutzkus, V, Kohl, DM, Rosa, GJ & Rutledge, JJ 2008 Mutations in the STAT5A gene are associated with embryonic survival and milk composition in cattle. Journal of Dairy Science 91 784–793Google Scholar
Lechner, J, Welte, T & Dopler, W 1997 Mechanism of interaction between the glucocorticoid receptor and STAT5: role of DNA-binding. Immunobiology 198 112–123CrossRefGoogle ScholarPubMed
Lin, JX, Mietz, J, Modi, WS, John, S & Leonard, WJ 1996 Cloning of human STAT5B. Reconstitution of interleukin-2-induced STAT5A and STAT5B DNA binding activity in COS-7 cells. Journal of Biological Chemistry 271 10738–10744CrossRefGoogle ScholarPubMed
Liu, X, Robinson, GW, Gouilleux, F, Groner, B & Hennighausen, L 1995 Cloning and expression of Stat5 and an additional homologue (Stat5b) involved in prolactin signal transduction in mouse mammary tissue. Proceeding of the National Academy of Sciences of the USA 92 8831–8835Google Scholar
McCracken, JY, Molenaar, AJ, Snell, RJ, Davey, HW & Wilkins, RJ 1997 A polymorphic TG repeat present within the bovine STAT5A gene. Animal Genetics 28 453–464CrossRefGoogle ScholarPubMed
Moleenar, A, Wheeler, TT, McCracken, JY & Seyfert, H 2000 The STAT3-encoding gene resides within the 40 kbp gap between the STAT5A- and STAT5B-encoding genes in cattle. Animal Genetics 31 333–346Google Scholar
Moriggl, R, Gouilleux-Gruart, V, Jahne, R, Berchtold, S, Gartmann, C, Liu, X, Hennighausen, L, Sotiropoulos, A, Groner, B & Gouilleux, F 1996 Deletion of the carboxyl-terminal transactivation domain of MGF-Stat5 results in sustained DNA binding and a dominant negative phenotype. Molecular and Cellular Biology 16 5691–5700Google Scholar
Morris, CA, Cullen, NG, Glass, BC, Hyndman, DL, Manley, TR, Hickey, SM, McEwan, JC, Pitchford, WS, Bottema, CD & Lee, MA 2007. Fatty acid synthase effects on bovine adipose fat and milk fat. Mammalian Genome 18 64–74CrossRefGoogle ScholarPubMed
Mui, AL, Wakao, H, O'Farrel, AM, Harada, N & Miyajima, A 1995 Interleukin-3, granulocyte-macrophage colony stimulating factor and interleukin-5 transduce signals through two STAT5 homologs. EMBO Journal 14 1166–1175Google Scholar
Pellegrini, S & Dusanter-Fourt, I 1997 The structure, regulation and function of the Janus kinases (JAKs) and signal transducers and activators of transcription (STATs). European Journal of Biochemistry 248 615–633Google Scholar
Ripperger, JA, Fritz, S, Richter, K, Hocke, GM, Lottspeich, F & Fey, GH 1995 Transcription factors Stat3 and Stat5b are present in rat liver nuclei late in an acute phase response and bind interleukin-6 response elements. Journal of Biological Chemistry 270 29998–30006CrossRefGoogle Scholar
Sadeghi, M, Moradi Shahrbabak, M, Rahimi Mianji, G & Nejati Javaremi, A 2009 Polymorphism at locus of STAT5A and its association with breeding values of milk production traits in Iranian Holstein bulls. Livestock Science 123 97–100CrossRefGoogle Scholar
Schindler, C & Darnell, JE Jr 1995 Transcriptional responses to polypeptide ligands. The JAK-STAT pathway. Annual Review of Biochemistry 64 621–651Google Scholar
Schrooten, C, Bovenhuis, H, Coppieters, W & van Arendonk, JAM 2000 Whole genome scan to detect quantitative trait loci for conformation and functional traits in dairy cattle. Journal of Dairy Science 83 795–806CrossRefGoogle ScholarPubMed
Seyfert, H, Pitra, C, Meyer, L, Brunner, RM, Wheeler, TT, Molenaar, A, McCracken, JY, Herrmann, J, Thiesen, H & Schwerin, M 2000 Molecular characterization of STAT5A- and STAT5B-encoding genes reveals extended intragenic sequence homogeneity in cattle and mouse and different degrees of divergent evolution of various domains. Journal of Molecular Evolution 50 550–561CrossRefGoogle ScholarPubMed
Silva, CM, Lu, H, Day, RN 1996 Characterization and cloning of STAT5 from IM-9 cells and its activation by growth hormone. Molecular Endocrinology 10 508–518Google ScholarPubMed
SAS User's Guide Statistics, Version 8.0 Edition 1999 SAS Inst. Inc. Cary NC, USAGoogle Scholar
Suguisawa, L, Souza, AA, Oliveira, HN, Curi, RA & Silveira, AC 2006. Relationship between growth hormone and STAT5A genes polymorphisms and beef cattle growth, carcass and meat quality traits. In: 8th World Congress on Genetics Applied to Livestock Production, August 13–18, 2006, Belo Horizonte, MG, BrazilGoogle Scholar
Suguisawa, L 2005 [Identification of superior genotypes for growth and carcass quality in beef cattle reared according to ‘young beef cattle production System’]. Tese (Doutorado em Zootecnia). Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, BotucatuGoogle Scholar
Verdier, F, Chretien, S, Muller, O, Varlet, P, Yoshimura, A, Gisselbrecht, S, Lacombe, C & Mayeux, P 1998 Proteasomes regulate erythropoietin receptor and signal transducer and activator of transcription 5 (STAT5) activation. Possible involvement of the ubiquitinated Cis protein. Journal of Biological Chemistry 273 28185–28190Google Scholar
Wakao, H, Gouilleux, F & Groner, B 1994 Mammary gland factor (MGF) is a novel member of the cytokine regulated transcription factor gene family and confers the prolactin response. EMBO Journal 13 2182–2191Google Scholar