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Functional analysis of the dairy cow mammary transcriptome between early lactation and mid-dry period

Published online by Cambridge University Press:  07 February 2019

Ye Lin ,
He Lv ,
Minghui Jiang ,
Jinyu Zhou ,
Shuyuan Song  and
Xiaoming Hou
Ye Lin
Affiliation:
Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin 150030, China
He Lv
Affiliation:
Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin 150030, China
Minghui Jiang
Affiliation:
Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, China
Jinyu Zhou
Affiliation:
Key Laboratory of Dairy Science of Education Ministry, Northeast Agricultural University, Harbin 150030, China
Shuyuan Song
Affiliation:
Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, China
Xiaoming Hou*
Affiliation:
Key Laboratory of Animal Cellular and Genetics Engineering of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, China
*
Author for correspondence: Xiaoming Hou, Email: [email protected]
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Abstract

In this research communication we used digital gene expression (DGE) analysis to identify differences in gene expression in the mammary glands of dairy cows between early lactation and the mid-dry period. A total of 741 genes were identified as being differentially expressed by DGE analysis. Compared with their expression in dry cows, 214 genes were up-regulated and 527 genes were down-regulated in lactating cow mammary glands. Gene Ontology analysis showed that lactation was supported by increased gene expression related to metabolic processes and nutrient transport and was associated with decreased gene expression related to cell proliferation. Pathway mapping using the Kyoto Encyclopedia of Genes and Genomes showed that 579 differentially expressed genes had pathway annotations related to 204 pathways. Metabolic pathway-related genes were the most significantly enriched. Genes and pathways identified by the present study provide insights into molecular events that occur in the mammary gland between early lactation and mid-dry period, which can be used to facilitate further investigation of the mechanisms underlying lactation and mammary tissue remodeling in dairy cows.

Keywords

Differential gene expressiondry periodlactationmammary gland
Type
Research Article
Information
Journal of Dairy Research , Volume 86 , Issue 1 , February 2019 , pp. 63 - 67
Copyright
Copyright © Hannah Dairy Research Foundation 2019 

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