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Factors in ruminant colostrum that influence cell growth and murine IgE antibody responses

Published online by Cambridge University Press:  01 June 2009

Dennis L. Watson ,
Geoffrey L. Francis  and
F. John Ballard
Dennis L. Watson
Affiliation:
CSLRO Division of Animal Health, Armidale, New South Wales 2350, Australia
Geoffrey L. Francis
Affiliation:
CSIRO Division of Human Nutrition, Kintore Avenue, Adelaide, South Australia 5000, Australia
F. John Ballard
Affiliation:
CSIRO Division of Human Nutrition, Kintore Avenue, Adelaide, South Australia 5000, Australia
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Summary

Bovine colostrum was investigated as a source of biologically active molecules capable of stimulating the growth of mammalian cells in culture and modifying the immune response in a murine model. An extract prepared from bovine colostral whey by cation exchange and re versed-phase chromatography stimulated the growth of L6 rat myoblasts, Balb/c-3T3 mouse fibroblasts and BHK-21 baby hamster kidney cells with equal or greater potency than fetal bovine serum. Fractionation of the bovine colostral extract by gel-permeation chromatography in M-acetic acid identified a number of cell-growth factors for each cell type. Bovine colostral extract was compared with an ovine colostral whey preparation for its ability to modulate IgE antibody responses in mice. Doses of 8 and 4 mg/d of ovine colostral whey or bovine colostral extract specifically suppressed IgE antibody responses, whereas at lower doses suppression did not occur. We conclude that bovine colostrum contains cell-growth factors as well as immunomodulatory factors that are able to regulate the IgE response in a heterologous species.

Type
Original Articles
Information
Journal of Dairy Research , Volume 59 , Issue 3 , August 1992 , pp. 369 - 380
Copyright
Copyright © Proprietors of Journal of Dairy Research 1992

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References

REFERENCES

Balfour, W. E. & Comline, R. S. 1962 Acceleration of the absorption of unchanged globulin in the new-born calf by factors in colostrum. Journal of Physiology 160 234–257CrossRefGoogle ScholarPubMed
Ballard, F. J., Nield, M. K., Francis, G. L., Dahlenburg, G. W. & Wallace, J. C. 1982 The relationship between the insulin content and inhibitory effects of bovine colostrum on protein breakdown in cultured cells. Journal of Cellular Physiology 110 249–254CrossRefGoogle ScholarPubMed
Ballard, F. J., Read, L. C., Francis, G. L., Bagley, C. J. & Wallace, J. C. 1986 Binding properties and biological potencies of insulin-like growth factors in L6 myoblasts. Biochemical Journal 233 223–230Google Scholar
Ballard, F. J., Ross, M., Upton, F. M. & Francis, G. L. 1988 Specific binding of insulin-like growth factors 1 and 2 to the type 1 and type 2 receptors respectively. Biochemical Journal 249 721–726CrossRefGoogle Scholar
Bradshaw, G. L., Sato, G. H., McClure, D. B. & Dubes, G. R. 1983 The growth requirements of BHK-21 cells in serum-free culture. Journal of Cellular Physiology 114 215–221CrossRefGoogle ScholarPubMed
Brambell, F. W. R. (Ed.) 1970 The Transmission of Passive Immunity from Mother to Young. London: North-Holland (Frontiers of Biology vol. 18)Google Scholar
Daniel, T. O., Tremble, P. M., Frackelton, A. R. & Williams, L. T. 1985 Purification of the platelet-derived growth factor receptor by using an anti-phosphotyrosine antibody. Proceedings of the National Academy of Sciences of the USA 82 2684–2687Google Scholar
Duncan, R. L. & McArthur, W. P. 1981 Lactoferrin-mediated modulation of mononuclear cell activities. 1. Suppression of the murine in vitro primary antibody response. Cellular Immunology 63 308–320Google Scholar
Francis, G. L., Read, L. C., Ballard, F. J., Bagley, C. J., Upton, F. M., Gravestock, P. M. & Wallace, J. C. 1986 Purification and partial sequence analysis of insulin-like growth factor-1 from bovine colostrum. Biochemical Journal 233 207–213CrossRefGoogle ScholarPubMed
Francis, G. L., Upton, F. M., Ballard, F. J., McNeil, K. A. & Wallace, J. C. 1988 Insulin-like growth factors 1 and 2 in bovine colostrum. Sequences and biological activities compared with those of a potent truncated form. Biochemical Journal 251 95–103CrossRefGoogle ScholarPubMed
Gooden, J. M. & Lascelles, A. K. 1971 Factor in colostrum causing mobilization of free fatty acid in newborn calves. Australian Journal of Experimental Biology and Medical Science 49 635–638CrossRefGoogle ScholarPubMed
Ishizaka, K. 1989 Regulation of immunoglobulin E biosynthesis. Advances in Immunology 47 1–44CrossRefGoogle ScholarPubMed
Jahnke, G. D. & Lazarus, L. H. 1984 A bombesin immunoreactive peptide in milk. Proceedings of the National Academy of Sciences of the USA 81 578–582CrossRefGoogle ScholarPubMed
Julius, M. H., Janusz, M. & Lisowski, J. 1988 A colostral protein that induces the growth and differentiation of resting B lymphocytes. Journal of Immunology 140 1366–1371Google Scholar
Juto, P. 1985 Human milk stimulates B cell function. Archives of Disease in Childhood 60 610–613CrossRefGoogle ScholarPubMed
Katona, I. M., Urban, J. F. & Finkelman, F. D. 1988 The role of L3T4+ and LYT-2+ T cells in the IgE response and immunity to Nippostrongylus brasiliensis. Journal of Immunology 140 3206–3211CrossRefGoogle ScholarPubMed
Kemeny, D. M. & Diaz-Sanchez, D. 1990 Can persistent IgE responses be suppressed? Clinical and Experimental Immunology 82 423–426CrossRefGoogle ScholarPubMed
Klagsbrun, M. 1980 Bovine colostrum supports the serum-free proliferation of epithelial cells but not of fibroblasts in long-term culture. Journal of Cell Biology 84 808–814CrossRefGoogle Scholar
Klagsbrun, M., Neumann, J. & Tapper, D. 1979 The mitogenic activity of human breast milk. Journal of Surgical Research 26 417–422CrossRefGoogle ScholarPubMed
Laemmli, U. K. 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 680–685CrossRefGoogle ScholarPubMed
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. 1951 Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193 265–275CrossRefGoogle ScholarPubMed
Oliver, M. H., Harrison, N. K., Bishop, J. E., Cole, P. J. & Laurent, G. J. 1989 A rapid and convenient assay for counting cells cultured in microwell plates: application for the assessment of growth factors. Journal of Cell Science 92 513–518CrossRefGoogle ScholarPubMed
Pabst, H. F. & Spady, D. W. 1990 Effect of breast-feeding on antibody response to conjugate vaccine. Lancet 336 269–270CrossRefGoogle ScholarPubMed
Ramirez, O. T., Sureshkumar, G. K. & Mutharasan, R. 1990 Bovine colostrum or milk as a serum substitute for the cultivation of a mouse hybridoma. Biotechnology and Bioengineering 35 882–889CrossRefGoogle ScholarPubMed
Roberts, S. A. & Freed, D. L. J. 1977 Neonatal IgA secretion enhanced by breast feeding. Lancet ii 1131CrossRefGoogle Scholar
Roldan, A., Charreau, E., Schillaci, R., Eugui, E. M. & Allison, A. C. 1989 Insulin-like growth factor- 1 increases the mitogenic response of human peripheral blood lymphocytes to phytohemagglutinin. Immunology Letters 20 5–8CrossRefGoogle ScholarPubMed
Sarfati, M., Vanderbeeken, Y., Rubio-Trujillo, M., Duncan, D. & Delespesse, O. 1986 Presence of IgE suppressor factors in human colostrum. European Journal of Immunology 16 1005–1008Google Scholar
Shinmoto, H., Kawakami, H., Dosako, S. & Sogo, Y. 1986 IgA specific helper factor (αHF) in human colostrum. Clinical and Experimental Immunology 66 228–230Google Scholar
Steimer, K. S., Packard, R., Holden, D. & Klagsbrun, M. 1981 The serum-free growth of cultured cells in bovine colostrum and in milk obtained later in the lactation period. Journal of Cellular Physiology 109 223–234CrossRefGoogle ScholarPubMed
Stuart, C. A., Meehan, R. T., Neale, L. S., Cintron, N. M. & Furlanetto, R. W. 1991 Insulin-like growth factor-1 binds selectively to human peripheral blood monocytes and B-lymphocytes. Journal of Clinical Endocrinology and Metabolism 72 1117–1122CrossRefGoogle ScholarPubMed
Takeyama, M., Wakayama, K., Takayama, F., Kondo, K., Fujii, N. & Yajima, H. 1990 Micro-enzyme immunoassay of vasoactive intestinal polypeptide (VIP)-like immunoreactive substance in bovine milk. Chemical and Pharmaceutical Bulletin 38 960–962CrossRefGoogle ScholarPubMed
Tamm, T. & Kikuchi, T. 1990 Insulin-like growth factor-1 (IGF-1), insulin, and epidermal growth factor (EGF) are survival factors for density-inhibited, quiescent Balb/c-3T3 murine fibroblasts. Journal of Cellular Physiology 143 494–500CrossRefGoogle ScholarPubMed
Thurston, A. W., Cole, J. A., Hillman, L. S., Im, J. H., Thorne, P. K., Krause, W. J., Jones, J. R., Eber, S. L. & Forte, L. R. 1990 Purification and properties of parathyroid hormone-related peptide isolated from milk. Endocrinology 126 1183–1190CrossRefGoogle ScholarPubMed
Watson, D. L. 1980 Immunological functions of the mammary gland and its secretion - a comparative review. Australian Journal of Biological Science 33 403–422CrossRefGoogle ScholarPubMed
Wilson, W. E., Lazarus, L. H. & Tomer, K. B. 1989 Bradykinin and kininogens in bovine milk. Journal of Biological Chemistry 264 17777–17783CrossRefGoogle ScholarPubMed