Hostname: page-component-cd9895bd7-gbm5v Total loading time: 0 Render date: 2024-12-23T10:23:15.706Z Has data issue: false hasContentIssue false
  • English
  • Français

Evaluation of Brix refractometer as an on-farm tool for colostrum IgG evaluation in Italian beef and dairy cattle

Published online by Cambridge University Press:  06 May 2021

Lorenzo Pisello ,
Claudio Forte ,
Nicoletta D'Avino ,
Rocco Pisano ,
Doreene Rose Hyatt ,
Fabrizio Rueca  and
Fabrizio Passamonti
Lorenzo Pisello*
Affiliation:
Department of Veterinary Medicine, University of Perugia, Via San Costanzo, 4, 06126, Perugia, Italy
Claudio Forte
Affiliation:
Department of Veterinary Sciences, University of Turin, Largo Paolo Braccini 2, 10095Grugliasco, Italy
Nicoletta D'Avino
Affiliation:
Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche ‘Togo Rosati’, Via Gaetano Salvemini, 1, 06126, Perugia, Italy
Rocco Pisano
Affiliation:
Department of Veterinary Medicine, University of Perugia, Via San Costanzo, 4, 06126, Perugia, Italy
Doreene Rose Hyatt
Affiliation:
Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA
Fabrizio Rueca
Affiliation:
Department of Veterinary Medicine, University of Perugia, Via San Costanzo, 4, 06126, Perugia, Italy
Fabrizio Passamonti
Affiliation:
Department of Veterinary Medicine, University of Perugia, Via San Costanzo, 4, 06126, Perugia, Italy
*
Author for correspondence: Lorenzo Pisello, Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

In this study it is hypothesized that there are differences between immunoglobulin G (IgG) content in colostrum from beef (Chianina, Podolica) and dairy (Holstein Friesian) cows and that variables such as breed, and parity can influence IgG content. The further objective was to determine if these factors may vary in terms of sensitivity, specificity and the cut point when data obtained with the digital Brix refractometer is compared with the gold standard radial immunodiffusion assay (RID). A total of 90 samples of first-milking colostrum were collected within 2 h after parturition. IgG concentration was determined indirectly by digital Brix refractometer and directly by RID. Results obtained by RID were compared among breed and parity. For the digital Brix refractometer, sensitivity and specificity to detect colostrum with an IgG concentration lower than 50 g/l were calculated and the optimal cut-point was selected for each breed. Samples containing less than <50 g/l IgG accounted for 15.9% of the total. Parity influenced colostral IgG concentration and beef cows had a higher mean concentration of IgG (101.1 g/l in Chianina and 90.6 g/l in Podolica) than dairy cows (71.1 g/l in Holstein Friesian) First parity Chianina cows had the highest IgG mean content (116.1 g/l). At the optimal cut-point for Brix refractometer (20%) sensitivity and specificity were 0.93 (0.84–0.97) and 0.81 (0.70–0.88), however, a breed-related cut-point could be used to reduce evaluation error. Linear regression modeling showed that refractometer data were related to RID (r = 0.78). Results obtained suggest that breed and parity can influence IgG content of colostrum and, despite the Brix refractometer being an excellent on-farm tool, a breed-based definition of optimal cut point is needed.

Keywords

Brixbeef cattlecut-pointimmunoglobulinradial immunodiffusion
Type
Research Article
Information
Journal of Dairy Research , Volume 88 , Issue 2 , May 2021 , pp. 189 - 193
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Baumrucker, CR, Burkett, AM, Magliaro-Macrina, AL and Dechow, CD (2010) Colostrogenesis: mass transfer of immunoglobulin G1 into colostrum. Journal of Dairy Science 93, 3031–3038.10.3168/jds.2009-2963CrossRefGoogle ScholarPubMed
Bielmann, V, Gillan, J, Perkins, NR, Skidmore, AL, Godden, S and Leslie, KE (2010) An evaluation of Brix refractometry instruments for measurement of colostrum quality in dairy cattle. Journal of Dairy Science 93, 3713–3721.10.3168/jds.2009-2943CrossRefGoogle ScholarPubMed
Conneely, M, Berry, DP, Sayers, R, Murphy, JP, Lorenz, I, Doherty, ML and Kennedy, E (2013) Factors associated with the concentration of immunoglobulin G in the colostrum of dairy cows. Animal: An International Journal of Animal Bioscience 7, 1824.10.1017/S1751731113001444CrossRefGoogle ScholarPubMed
Earley, B, Tiernan, K, Duffy, C and McGee, M (2018) Effect of suckler cow vaccination against glycoprotein E (gE)-negative bovine herpesvirus type 1 (BoHV-1) on passive immunity and physiological response to subsequent bovine respiratory disease vaccination of their progeny. Research in Veterinary Science 118, 43–51.CrossRefGoogle Scholar
Elsohaby, I, Windeyer, MC, Haines, DM and Keefe, GP (2018) Application of transmission infrared spectroscopy and partial least squares regression to predict immunoglobulin G concentration in dairy and beef cow colostrum. Journal of Animal Science 96, 771–782.10.1093/jas/sky003CrossRefGoogle ScholarPubMed
Fleenor, WA and Stott, GH (1980) Hydrometer test for estimation of immunoglobulin concentration in bovine colostrum. Journal of Dairy Science 63, 973–977.10.3168/jds.S0022-0302(80)83034-7CrossRefGoogle ScholarPubMed
Fox, PF, Uniacke-Lowe, T, McSweeney, PLH and O'Mahony, JA (2015) Chemistry and biochemistry of cheese. Dairy Chemistry and Biochemistry. Cham: Springer, pp. 499–546.CrossRefGoogle Scholar
Godden, S (2008) Colostrum management for dairy calves. Veterinary Clinics of North America – Food Animal Practice 24, 19–39.CrossRefGoogle ScholarPubMed
Gulliksen, SM, Lie, KI, Sølverød, L and Østerås, O (2008) Risk factors associated with colostrum quality in Norwegian dairy cows. Journal of Dairy Science 91, 704–712.10.3168/jds.2007-0450CrossRefGoogle ScholarPubMed
Guy, MA, McFadden, TB, Cockrell, DC and Besser, TE (1994) Regulation of colostrum formation in beef and dairy cows. Journal of Dairy Science 77, 3002–3007.CrossRefGoogle ScholarPubMed
Homerosky, ER, Timsit, E, Pajor, EA, Kastelic, JP and Windeyer, MC (2017) Predictors and impacts of colostrum consumption by 4 h after birth in newborn beef calves. Veterinary Journal 228, 1–6.CrossRefGoogle Scholar
Jaster, EH (2005) Evaluation of quality, quantity, and timing of colostrum feeding on immunoglobulin G1 absorption in Jersey calves. Journal of Dairy Science 88, 296–302.CrossRefGoogle ScholarPubMed
Kehoe, SI, Heinrichs, AJ, Moody, ML, Jones, CM and Long, MR (2011) Comparison of immunoglobulin G concentrations in primiparous and multiparous bovine colostrum. The Professional Animal Scientist 27, 176–180.10.15232/S1080-7446(15)30471-XCrossRefGoogle Scholar
McGee, M and Earley, B (2018) Review: passive immunity in beef-suckler calves. Animal: An International Journal of Animal Bioscience 13, 810–825.CrossRefGoogle ScholarPubMed
McGee, M, Drennan, MJ and Caffrey, PJ (2005) Effect of suckler cow genotype on cow serum immunoglobulin (Ig) levels, colostrum yield, composition and Ig concentration and subsequent immune status of their progeny. Irish Journal of Agricultural and Food Research 44, 173–183.Google Scholar
McGee, M, Drennan, MJ and Caffrey, PJ (2006) Effect of age and nutrient restriction pre partum on beef suckler cow serum immunoglobulin concentrations, colostrum yield, composition and immunoglobulin concentration and immune status of their progeny. Irish Journal of Agricultural and Food Research 45, 157–171.Google Scholar
McGuirk, SM and Collins, M (2004) Managing the production, storage, and delivery of colostrum. Veterinary Clinics: Food Animal Practice 20(3), 593–603.Google ScholarPubMed
Moretti, DB, Santos, CB, Alencar, SM and Machado-Neto, R (2020) Colostrum from primiparous Holstein cows shows higher antioxidant activity than colostrum of multiparous ones. Journal of Dairy Research 87, 356–359.CrossRefGoogle ScholarPubMed
Morin, DE, Nelson, SV, Reid, ED, Nagy, DW, Dahl, GE and Constable, PD (2010) Effect of colostral volume, interval between calving and first milking, and photoperiod on colostral IgG concentrations in dairy cows. Journal of the American Veterinary Medical Association 237, 420–428.CrossRefGoogle ScholarPubMed
Nocek, JE, Braund, DG and Warner, RG (1984) Influence of neonatal colostrum administration, immunoglobulin, and continued feeding of colostrum on calf gain, health, and serum protein. Journal of Dairy Science 67, 319–333.10.3168/jds.S0022-0302(84)81305-3CrossRefGoogle ScholarPubMed
Quigley, JD, Lago, A, Chapman, C, Erickson, P and Polo, J (2013) Evaluation of the Brix refractometer to estimate immunoglobulin G concentration in bovine colostrum. Journal of Dairy Science 96, 1148–1155.CrossRefGoogle ScholarPubMed
Silva-del-Río, N, Rolle, D, García-Muñoz, A and Pandey, P (2017) Colostrum immunoglobulin G concentration of multiparous Jersey cows at first and second milking is associated with parity, colostrum yield, and time of first milking, and can be estimated with Brix refractometry. Journal of Dairy Science 100, 5774–5781.CrossRefGoogle ScholarPubMed
Vandeputte, S, Detilleux, J and Rollin, F (2014) Investigation of colostrum quality in beef cattle by radial immunodiffusion and brix refractometry. Veterinary Record 175, 353.CrossRefGoogle ScholarPubMed
Vann, RC, Holloway, JW, Carstens, GE, Boyd, ME and Randel, RD (1995) Influence of calf genotype on colostral immunoglobulins in Bos taurus and Bos indicus cows and serum immunoglobulins in their calves. Journal of Animal Science 73, 3044–3050.CrossRefGoogle ScholarPubMed
Supplementary material: PDF

Pisello et al. supplementary material

Pisello et al. supplementary material

Download Pisello et al. supplementary material(PDF)
PDF 102.8 KB