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Emulsifying capacity of whey proteins produced by ion-exchange chromatography

Published online by Cambridge University Press:  01 June 2009

Keith R. Langley ,
David Millard  and
E. William Evans
Keith R. Langley
Affiliation:
AFRC Institute of Food Research, Reading Laboratory, Shinfield, Reading RG2 9AT, UK
David Millard
Affiliation:
AFRC Institute of Food Research, Reading Laboratory, Shinfield, Reading RG2 9AT, UK
E. William Evans
Affiliation:
AFRC Institute of Food Research, Reading Laboratory, Shinfield, Reading RG2 9AT, UK
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Summary

At pH 7·0, the emulsifying capacity (Ec) of ion-exchange fractionated whey protein powders in corn oil/water systems increased with increase in protein concentration up to 0·l% (w/v), where it was maximal. At higher concentrations Ec decreased because of packing effects of the oil droplets. At low protein concentrations Ec could be determined from linear equations involving the relative concentration of the individual proteins in the powder. The surface coverage of β-lactoglobulin on the oil was ∼ 1 mg m–2. Oil droplet size and Ec were maximal at pH 4·0–6·0, corresponding to the isoionic points of the proteins.

Type
Original articles
Information
Journal of Dairy Research , Volume 55 , Issue 2 , May 1988 , pp. 197 - 203
Copyright
Copyright © Proprietors of Journal of Dairy Research 1988

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References

REFERENCES

Acton, J. C. & Saffle, R. L. 1972 Emulsifying capacity of muscle protein: phase volumes at emulsion collapse. Journal of Food Science 37 904–906CrossRefGoogle Scholar
Andrews, A. T., Taylor, M. D. & Owen, A. J. 1985 Rapid analysis of bovine milk proteins by fast protein liquid chromatography. Journal of Chromatography 348 177–185CrossRefGoogle ScholarPubMed
Crenwelge, D. D., Dill, C. W., Tybor, P. T. & Landmann, W. A. 1974 A comparison of the emulsification capacities of some protein concentrates. Journal of Food Science 39 175–177CrossRefGoogle Scholar
Delaney, R. A. M. 1976 Composition, properties and uses of whey protein concentrates. Journal of the Society of Dairy Technology 29 91–101CrossRefGoogle Scholar
Fox, P. F. & Mulvihill, D. M. 1982 Milk proteins: molecular, colloidal and functional properties. Journal of Dairy Research 49 679–693CrossRefGoogle Scholar
Gekko, K. & Noguchi, H. 1979 Compressibility of globular proteins in water at 25 °C. Journal of Physical Chemistry 83 2706–2714CrossRefGoogle Scholar
Halling, P. J. 1981 Protein-stabilized foams and emulsions. CRC Critical Reviews in Food Science and Nutrition 15 155–203CrossRefGoogle ScholarPubMed
Harper, W. J. 1984 Model food system approaches for evaluating whey protein functionality. Journal of Dairy Science 67 2745–2756CrossRefGoogle Scholar
Hillier, R. M. 1976 The quantitative measurement of whey proteins using polyacrylamide-gel electro-phoresis. Journal of Dairy Research 43 259–265CrossRefGoogle Scholar
Kilara, A. 1984 Standardization of methodology for evaluating whey proteins. Journal of Dairy Science 67, 2734–2744CrossRefGoogle Scholar
Kuehler, C. A. & Stine, C. M. 1974 Effect of enzymatic hydrolysis on some functional properties of whey protein. Journal of Food Science 39 379–382CrossRefGoogle Scholar
Langley, K. R., Millard, D. & Evans, E. W. 1986 Determination of tensile strength of gels prepared from fractionated whey proteins. Journal of Dairy Research 53 285–292CrossRefGoogle Scholar
Oortwijn, H. & Walstra, P. 1979 The membranes of recombined fat globules. 2. Composition. Netherlands Milk and Dairy Journal 33 134–154Google Scholar
Oohtwijn, H., Walstra, P. & Mulder, H. 1977 The membranes of recombined fat globules. 1. Electron microscopy. Netherlands Milk and Dairy Journal 31 134–147Google Scholar
Pearson, A. M., Spooner, M. E., Hegarty, G. R. & Bratzler, L. J. 1965 The emulsifying capacity and stability of soy sodium proteinate, potassium caseinate and nonfat dry milk. Food Technology 19 1841–1845Google Scholar
Shimizu, M., Kamiya, T. & Yamauchi, K. 1981 The adsorption of whey proteins on the surface of emulsified fat. Agricultural and Biological Chemistry 45 2491–2496Google Scholar
Skudder, P. J. 1985 Evaluation of a porous silica-based ion-exchange medium for the production of protein fractions from rennet- and acid-whey. Journal of Dairy Research 52 167–181CrossRefGoogle Scholar
Tornberg, E. & Hermansson, A.-M. 1977 Functional characterization of protein stabilized emulsions: effect of processing. Journal of Food Science 42 468–472CrossRefGoogle Scholar
Webb, N. B., Ivey, F. J., Craig, H. B., Jones, V. A. & Monroe, R. J. 1970 The measurement of emulsifying capacity by electrical resistance. Journal of Food Science 35 501–504CrossRefGoogle Scholar
Whitney, R. McL., Brunner, J. R., Ebner, K., Farrell, H. M., Josephson, R. V., Morr, G. V. & Swaisgood, H. E. 1976 Nomenclature of the proteins of cow's milk: Fourth revision. Journal of Dairy Science 59 795–815CrossRefGoogle ScholarPubMed
Yamauchi, K., Shimizu, M. & Kamiya, T. 1980 Emulsifying properties of whey protein. Journal of Food Science 45 1237–1242CrossRefGoogle Scholar