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Effects of Lactobacillus rhamnosus LA68 on the immune system of C57BL/6 mice upon oral administration

Published online by Cambridge University Press:  24 February 2014

Rajna Dimitrijevic ,
Nevena Ivanovic ,
Geir Mathiesen ,
Vladimir Petrusic ,
Irena Zivkovic ,
Brizita Djordjevic  and
Ljiljana Dimitrijevic
Rajna Dimitrijevic*
Affiliation:
Department of Research and Development, Institute of Virology, Vaccines and Sera, Torlak, Belgrade, Serbia
Nevena Ivanovic
Affiliation:
Department of Bromatology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia
Geir Mathiesen
Affiliation:
Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Aas, Norway
Vladimir Petrusic
Affiliation:
Department of Research and Development, Institute of Virology, Vaccines and Sera, Torlak, Belgrade, Serbia
Irena Zivkovic
Affiliation:
Department of Research and Development, Institute of Virology, Vaccines and Sera, Torlak, Belgrade, Serbia
Brizita Djordjevic
Affiliation:
Department of Bromatology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia
Ljiljana Dimitrijevic
Affiliation:
Department of Research and Development, Institute of Virology, Vaccines and Sera, Torlak, Belgrade, Serbia
*
*For correspondence; e-mail: [email protected]
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Abstract

Probiotic bacteria have been used in human nutrition for centuries and are now attracting more attention. In order to examine the immunological aspects of probiotic consumption, Lactobacillus rhamnosus LA68 was orally administrated using gavage to healthy C57BL/6 mice. After one month splenocytes were isolated, and analysed by flow cytometry. The magnitude of splenocyte proliferation upon stimulation with lipopolysaccharide and peptidoglycan and cytokine levels (IFN-γ, IL-6, IL-10 and IL-17) was assessed. Cytokine levels in the serum were also analysed. Oral application of strain LA68 leads to a significant decrease of CD3+, CD25+ and CD19+ cells, and an increase of CD11b+ and CD16/CD32+ positive cell populations in the mouse spleen. Increased sensitivity to stimulation through proliferation and IL-6 secretion was detected. Increased serum IFN-γ and decreased IL-10 levels were found. Our results show increased responsiveness of splenocytes, activation of the Th1 type of immune response, and a shift of leucocyte populations towards monocyte/granulocyte populations.

Keywords

Lactobacillus rhamnosus LA68C57BL/6 miceoral administrationleucocyte population shiftserum cytokines
Type
Research Article
Information
Journal of Dairy Research , Volume 81 , Issue 2 , May 2014 , pp. 202 - 207
Copyright
Copyright © Proprietors of Journal of Dairy Research 2014 

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