Hostname: page-component-cd9895bd7-p9bg8 Total loading time: 0 Render date: 2024-12-23T13:59:56.843Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of experimental challenge of ewes with Mannheimia haemolytica on subsequent milk composition

Published online by Cambridge University Press:  04 August 2008

Ilectra A Fragkou ,
Nikos Solomakos ,
Mark P Dagleish ,
Peter J Cripps ,
Nikos Papaioannou ,
Costas M Boscos ,
Haris N Ververidis ,
Charalambos Billinis ,
Denise C Orfanou  and
Alexander Govaris
...Show all authors
Ilectra A Fragkou
Affiliation:
Veterinary Faculty, University of Thessaly, Karditsa, Greece
Nikos Solomakos
Affiliation:
Veterinary Faculty, University of Thessaly, Karditsa, Greece
Mark P Dagleish
Affiliation:
Moredun Research Institute, Edinburgh, Scotland
Peter J Cripps
Affiliation:
Faculty of Veterinary Science, University of Liverpool, Neston, South Wirral, England
Nikos Papaioannou
Affiliation:
School of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece
Costas M Boscos
Affiliation:
School of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece
Haris N Ververidis
Affiliation:
Veterinary Faculty, University of Thessaly, Karditsa, Greece
Charalambos Billinis
Affiliation:
Veterinary Faculty, University of Thessaly, Karditsa, Greece
Denise C Orfanou
Affiliation:
Veterinary Faculty, University of Thessaly, Karditsa, Greece
Alexander Govaris
Affiliation:
Veterinary Faculty, University of Thessaly, Karditsa, Greece
Ilias Kyriazakis
Affiliation:
Veterinary Faculty, University of Thessaly, Karditsa, Greece
George C Fthenakis*
Affiliation:
Veterinary Faculty, University of Thessaly, Karditsa, Greece
*
*For correspondence; e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The objective was to describe the physicochemical changes during the early phase of subclinical mastitis and to associate them with pathological findings. A Mannheimia haemolytica strain was deposited into one teat duct of 25 ewes and the clinical, bacteriological, cytological, physicochemical (pH, milk composition), gross-pathological and histological findings were subsequently recorded. The organism was consistently isolated from samples of teat duct material (140/150) but not from mammary secretion (50/150). California Mastitis Test (CMT) scores increased (>1) and remained high (143/150 samples) after challenge; polymorphonuclear neutrophils (PMN) predominated in milk films, but the proportion of lymphocytes and macrophages progressively increased. Increased pH values (>7·0) were recorded in the mammary secretion from the challenged side. Furthermore, content of fat, total proteins and lactose therein decreased markedly. Histological changes (leucocytic infiltration, destruction of epithelial cells) were observed in the mammary parenchyma of the ewes. The present results confirm that the reduction of milk constituents is the effect of cellular damage and can occur soon after infection.

Keywords

MastitissheepteatMannheimia haemolytica
Type
Research Article
Information
Journal of Dairy Research , Volume 75 , Issue 3 , August 2008 , pp. 340 - 346
Copyright
Copyright © Proprietors of Journal of Dairy Research 2008

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Angen, O, Mutters, R, Caugant, DA, Olsen, JE & Bisgaard, M 1999 Taxonomic relationships of the [Pasteurella] haemolytica complex as evaluated by DNA-DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. nov., comb. nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida sp. nov., Mannheimia ruminalis sp. nov., and Mannheimia varigena sp. nov. International Journal of systematic Bacteriology 49 67–86Google ScholarPubMed
Baeker, R, Haebel, S, Schlatterer, K & Schlatterer, B 2002 Lipocalin-type prostaglandin D synthase in milk: a new biomarker for bovine mastitis. Prostaglandins & Other Lipid Mediators 67 75–88CrossRefGoogle ScholarPubMed
Barrow, GI & Feltham, RKA 1993 Manual for the Identification of Medical Bacteria, 3rd Edition. Cambridge, UK: Cambridge University PressGoogle Scholar
Bergonier, D & Berthelot, X 2001 New advances in epizootiology and control of ewe mastitis. Livestock Production Science 79 1–16CrossRefGoogle Scholar
Burriel, AR 1997a Dynamics of intramammary infection in the sheep caused by coagulase-negative staphylococci and its influence on udder tissue and milk composition. Veterinary Record 140 419–423CrossRefGoogle ScholarPubMed
Burriel, AR 1997b Evidence of breed susceptibility to experimentally produced ovine subclinical mastitis. Sheep and Goat Research Journal 13 20–23Google Scholar
El-Masannat, ETS, Jones, JET & Scott, MJ 1991 The experimental production of mastitis in sheep by intramammary inoculation of Pasteurella haemolytica. Journal of Comparative Pathology 105 455–465CrossRefGoogle ScholarPubMed
Euzeby, JP 1997 List of bacterial names with standing in nomenclature: a folder available on the internet. International Journal of Systematic Bacteriology 47 590–592 (List of Prokaryotic Names with Standing in Nomenclature)Google ScholarPubMed
Fragkou, IA, Mavrogianni, VS, Cripps, PJ, Gougoulis, DA & Fthenakis, GC 2007a The bacterial flora in the teat duct of ewes can protect against and can cause mastitis. Veterinary Research 38 525–545CrossRefGoogle ScholarPubMed
Fragkou, IA, Skoufos, J, Cripps, PJ, Kyriazakis, I, Papaioannou, N, Boscos, CM, Tzora, A & Fthenakis, GC 2007b Differences in susceptibility to Mannheimia haemolytica-associated mastitis between two breeds of dairy sheep. Journal of Dairy Research 74 349–355CrossRefGoogle ScholarPubMed
Fthenakis, G 1988 Ovine Mastitis with Special Reference to Subclinical Mastitis associated with Coagulase-negative Staphylococci. PhD thesis, University of LondonGoogle Scholar
Fthenakis, GC 1994 Prevalence and aetiology of subclinical mastitis in ewes of Southern Greece. Small Ruminant Research 13 293–300CrossRefGoogle Scholar
Fthenakis, GC 1995 California Mastitis Test and Whiteside Test in diagnosis of subclinical mastitis of dairy ewes. Small Ruminant Research 16 271–276CrossRefGoogle Scholar
Fthenakis, GC & Jones, JET 1990 The effect of experimentally induced subclinical mastitis on milk yield of ewes and on the growth of lambs. British Veterinary Journal 146 43–49CrossRefGoogle ScholarPubMed
Gougoulis, DA, Kyriazakis, I, Papaioannou, N, Papadopulos, E, Taitzoglou, IA & Fthenakis, GC 2008 Effects of subclinical mastitis on patterns of maternal—offspring behaviour of dairy sheep. Veterinary Journal doi:10.1016/j.tvjl.2007.02.024CrossRefGoogle Scholar
Leitner, G, Chaffer, M, Caraso, Y, Ezra, E, Kababea, D, Winkler, M, Glickman, A & Saran, A 2003 Udder infection and milk somatic cell count, NAGase activity and milk composition – fat, protein and lactose – in Israeli-Assaf and Awassi sheep. Small Ruminant Research 49 157–164CrossRefGoogle Scholar
Kwok, S & Higuchi, R 1989 Avoiding false positives with PCR. Nature 339 237–238CrossRefGoogle ScholarPubMed
Leitner, G, Chaffer, M, Shamay, A, Shapiro, F, Merin, U, Ezra, E, Saran, A & Silanikove, N 2004 Changes in milk composition as affected by subclinical mastitis in sheep. Journal of Dairy Science 87 46–52CrossRefGoogle ScholarPubMed
Mavrogianni, VS, Cripps, PJ & Fthenakis, GC 2006a Description and validation of a novel technique to study the bacterial flora of the teat duct of ewes. Small Ruminant Research 66 258–264CrossRefGoogle Scholar
Mavrogianni, VS, Cripps, PJ, Papaioannou, N, Taitzoglou, I, Fthenakis, GC 2006b Teat disorders predispose ewes to clinical mastitis after challenge with Mannheimia haemolytica. Veterinary Research 37 89–105CrossRefGoogle ScholarPubMed
Mavrogianni, VS, Fthenakis, GC, Brooks, H, Papaioannou, N, Cripps, PJ, Taitzoglou, I, Brellou, G & Saratsis, P 2005 The effects of inoculation of Mannheimia haemolytica into the teat of lactating ewes. Veterinary Research 36 13–25CrossRefGoogle ScholarPubMed
Miles, AA & Misra, JS 1938 The estimation of the bactericidal power of the blood. Journal of Hygiene, Cambridge 38 732–749Google ScholarPubMed
Persson-Waller, K, Colditz, IG & Seow, HF 1997 Accumulation of leucocytes and cytokines in the lactating ovine udder during mastitis due to Staphylococcus aureus and Escherichia coli. Research in Veterinary Science 62 63–66CrossRefGoogle ScholarPubMed
Pyörälä, S 2003 Indicators of inflammation in the diagnosis of mastitis. Veterinary Research 34 565–578CrossRefGoogle Scholar
Saratsis, Ph, Alexopoulos, C, Tzora, A & Fthenakis, GC 1999 The effect of experimentally induced subclinical mastitis on the milk yield of dairy ewes. Small Ruminant Research 32 205–209CrossRefGoogle Scholar
Santos, RA, Mendonca, CL, Alfonso, JAB & Simao, LCV 2007 Clinical aspects and characteristics of the milk in sheep with mastitis experimentally induced with Staphylococcus aureus. Pesquisa Veterinaria Brasileira 27 6–12CrossRefGoogle Scholar
Van Rensburg, E, Du Preez, JC & Ellis, CE 2006 Quantification of Mannheimia haemolytica leukotoxin by indirect ELISA. Onderstepoort Journal of veterinary Research 73 241–250Google ScholarPubMed