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Effect of varying enzyme concentration on the action of rennin on whole milk

Published online by Cambridge University Press:  01 June 2009

A. V. Castle  and
J. V. Wheelock
A. V. Castle
Affiliation:
School of Biological Sciences, The University, Bradford, Yorkshire BD7 1DP
J. V. Wheelock
Affiliation:
School of Biological Sciences, The University, Bradford, Yorkshire BD7 1DP
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Summary

The kinetics of the release of glycopeptides by the action of rennin on bovine whole milk have been studied. The initial rate of release of glycopeptides was proportional to the concentration of rennin. Using the integrated form of the Michaelis–Menten equation, values have been obtained for Km and V. These values varied between milk samples from individual cows, and for a single milk sample the value for Km increased with increasing rennin concentration. It is suggested that the Km value is partly dependent on the carbohydrate composition of individual κ-casein molecules.

Type
Research Article
Information
Journal of Dairy Research , Volume 39 , Issue 1 , February 1972 , pp. 15 - 22
Copyright
Copyright © Proprietors of Journal of Dairy Research 1972

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