Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-26T22:06:26.430Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of somatic cell counts on ewes' milk protein profile and cheese-making properties in different sheep breeds reared in Spain

Published online by Cambridge University Press:  13 March 2009

Isabel Revilla ,
José M Rodríguez-Nogales  and
Ana M Vivar-Quintana
Isabel Revilla*
Affiliation:
Food Technology Area, Universidad de Salamanca. E.P.S. de Zamora. Avda. Requejo 33, 49022, Zamora. Spain
José M Rodríguez-Nogales
Affiliation:
Food Technology Area, University of Valladolid.E.T.S. de Ingenierías Agrarias. Avda. Madrid 57, 34004 Palencia. Spain
Ana M Vivar-Quintana
Affiliation:
Food Technology Area, Universidad de Salamanca. E.P.S. de Zamora. Avda. Requejo 33, 49022, Zamora. Spain
*
*For correspondence; e-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Bulk tank ewe's milks from Assaf, Castellana and Churra breeds categorized within three different Somatic Cell Count (SCC) groups (LSCC: <500 000; MSCC: 1 000 000 to 1 500 000; and HSCC: 2 500 000 to 3 000 000 cells ml−1) were used to investigate changes in capillary electrophoresis protein profiles and cheese-making properties. The results do not reveal a significant effect of SCC on total casein contents, because the sum of β-caseins decreased as SCC increased; no statistically significant differences were observed for the sum of α-caseins, and the values of κ-casein were higher in the HSCC milk. However, the soluble proteins other than α-lactalbumin and β-lactoglobulin increased with SCC. Regarding the effect of breed, the Assaf breed had the lowest contents of κ-CN, αs1-I-CN, αs1-II-CN 1-CN, αs1-III-CN, β1-CN and β2-CN. The protein profile was significantly correlated with curd textural properties. αs1-I-CN was the most influential variant because it was positively correlated with a large number of textural parameters. Cheese yield was positively correlated with all casein variants except αs1-III-CN, showing that the milk from local breeds were more suitable for cheese-making due to their higher contents of all the casein variants. Regarding curd texture properties LSCC milk curds showed more cohesiveness, associated with its lower content of αs1-III-CN and Castellana milk curds showed the highest values for firmness owing to their higher content of αs1-I-CN.

Keywords

Capillary electrophoresistextureyieldSCCbreed
Type
Research Article
Information
Journal of Dairy Research , Volume 76 , Issue 2 , May 2009 , pp. 210 - 215
Copyright
Copyright © Proprietors of Journal of Dairy Research 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Albenzio, M, Caroprese, M, Santillo, A, Marino, R, Muscio, A & Sevi, A 2005 Proteolytic patterns and plasmin activity in ewes' milk as affected by somatic cell count and stage of lactation. Journal of Dairy Research 72 86–92CrossRefGoogle ScholarPubMed
Albenzio, M, Caroprese, M, Santillo, A, Marino, R, Taibi, L & Sevi, A 2004 Effects of somatic cell count and stage of lactatin on the plasmin activity and cheese-making properties of ewe milk. Journal of Dairy Science 87 533–542CrossRefGoogle Scholar
Barron, LJR, Redondo, Y, Flanagan, CE, Pérez-Elortondo, FJ, Albisu, M, Nájera, AI, de Renobales, M & Fernández-García, E 2005 Comparison of the volatile composition and sensory characteristics of Spanish PDO cheeses manufactures from ewes' raw milk and animal rennet. International Dairy Journal 15 371–382CrossRefGoogle Scholar
B.O.E. (Boletin Oficial del Estado) [State Official Bulletin] 1993 Order of 6-05-1993 of Ministry of Agriculture, Fish and Food that approves the Regulation board of Queso Zamorano Quality Appellation of Origin and the Regulation CouncilGoogle Scholar
Bencini, R & Pulina, G 1997 The quality of sheep milk: A review. Australian Journal of Experimental Agriculture 37 485–504CrossRefGoogle Scholar
Bianchi, L, Bolla, A, Budelli, E, Caroli, A, Casoli, C, Pauselli, M & Duranti, E 2004 Effect of udder health and lactation phase on the characteristics of Sardinian ewe milk. Journal of Dairy Science 87 2401–2408CrossRefGoogle ScholarPubMed
Bourne, MC 1978 Texture profile analysis. Food Technology 32(7) 62–66Google Scholar
Considine, T, Geary, S, Kelly, AL & McSweeney, PLH 2002 Proteolytic specificity of cathepsin G on bovine αs1- and β-caseins. Food Chemistry 76 59–67CrossRefGoogle Scholar
Considine, T, Healy, A, Kelly, AL & McSweeney, PLH 2000 Proteolytic specificity of elastase on bovine αs1-casein. Food Chemistry 69 19–26CrossRefGoogle Scholar
Considine, T, Healy, A, Kelly, AL & McSweeney, PLH 2004 Hydrolysis of bovine caseins by cathepsin B, a cysteine proteinase indigenous to milk. International Dairy Journal 14 117–124CrossRefGoogle Scholar
Duranti, E & Casoli, C 1991 Variazione della composizione azotata e dei parametri lattodinamografici del late di pecor in funzione del contenuto di cellule somatiche [Variations in the nitrogen composition and in the lactodinamographic parameters of ewe's milk in relation to somatic cell content]. Zootecnia e Nutrizione Animale 17 99–105Google Scholar
El-Saied, UM, Carriedo, JA & San Primitivo, F 1998 Heritability of test day somatic cell counts and its relationship with milk yield and protein percentage in dairy ewes. Journal of Dairy Science 81 2956–2961CrossRefGoogle ScholarPubMed
Galina, MA, Morales, R, Lopez, B & Carmona, MA 1996 Effect of somatic cell count on lactation and soft cheese yield by dairy goats. Small Ruminant Research 21 251–257CrossRefGoogle Scholar
González-Rodríguez, M, Gonzalo, C, San Primitivo, F & Cármenes, P 1995 Relationship between somatic cell count and intramammary infection of the half udder in dairy ewes. Journal of Dairy Science 78 2753–2759CrossRefGoogle ScholarPubMed
Gonzalo, C, Carriedo, JA, Blanco, MA, Beneitez, E, Juárez, MT, De la Fuente, LF & San Primitivo, F 2005 Factors of variation influencing bulk tank Somatic Cell Count in dairy sheep. Journal of Dairy Science 88 969–974CrossRefGoogle ScholarPubMed
Jaeggi, JJ, Govindasamy-Lucey, S, Berger, YM, Johnson, ME, McKusick, BC, Thomas, DL & Wendorff, WL 2003 Hard ewe's milk cheese manufactured from milk of three different groups of somatic cell counts. Journal of Dairy Science 86 3082–3089CrossRefGoogle ScholarPubMed
Jaubert, G, Gay Jacquin, MF & Perrin, G 1996 Numérations cellulaires et caractéristiques biochimiques et technologiques du lait de chévre [Somatic cell counts and biochemical and technological characteristics of goat milk]. In Proceedings of the International Symposium on Somatic Cells and Milk of Small Ruminants, pp. 263–268 (Ed. Rubino, R). EAAP Publication No. 77, Wageningen PressGoogle Scholar
Kalantzopoulos, G, Dubeuf, JP, Vallerand, F, Pirisi, A, Casalta, E, Laurte, A & Trujillo, T 2004 Characteritics of sheep and goat milks: quality and hygienic factors for the sheep and goat dairy sectors. Bulletin of IDF 390 17–28Google Scholar
Leitner, G, Chaffer, M, Caraso, Y, Ezra, E, Kababea, D, Winkler, M, Winkler, M, Glickman, A & Saran, A 2003 Udder infection and milk somatic cell count, NAGase activiy and milk composition-fat, protein and lactose- in Israeli-Assaf and Awassi sheep. Small Ruminant Research 49 157–164CrossRefGoogle Scholar
Marino, R, Considine, T, Sevi, A, McSweeney, PLH & Kelly, AL 2005 Contribution of proteolytic activity associated with somatic cells in milk to cheese ripening. International Dairy Journal 15 1026–1033CrossRefGoogle Scholar
Nudda, A, Feligini, M, Battacone, G, Macciota, NPP & Pulina, G 2003 Effects of lactation stage, parity, β-lactoglobulin genotype and milk SCC on whey protein composition in Sarda dairy ewes. Italian Journal of Animal Science 2 29–39CrossRefGoogle Scholar
Pirisi, A, Piredda, G, Corona, M, Pes, M, Pintus, S & Ledda, A 2000 Influence of somatic cell count on ewe's milk composition, cheese yield and cheese quality. In Proceedings of 6th Great Lakes Dairy Sheep Symposium, pp. 47–59. Ontario, CanadaGoogle Scholar
Raynal-Ljutovac, K, Pirisi, A, de Crémoux, R & Gonzalo, C 2007 Somatic cells of goat and sheep milk: Analytical, sanitary, productive and technological aspects. Small Ruminant Research 68 126–144CrossRefGoogle Scholar
Revilla, I, Rodríguez-Nogales, JM & Vivar-Quintana, AM 2007 Proteolysis and texture of hard ewes' milk cheese during ripening as affected by somatic cell counts. Journal of Dairy Research 74 127–136CrossRefGoogle ScholarPubMed
Rodríguez-Nogales, JM, Vivar-Quintana, AM & Revilla, I 2007 Influence of somatic cell count and breed on capillary electrophoretic protein profiles of ewes' milk: A chemometric study. Journal of Dairy Science 90 3186–3196CrossRefGoogle ScholarPubMed
Verdi, RJ, Barbano, DM, DellaValle, ME & Senyk, GF 1987 Variability in the protein, casein, nonprotein nitrogen, and proteolysis in high and low somatic cell milks. Journal of Dairy Science 70 230–242CrossRefGoogle ScholarPubMed