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Effect of insulin in conjunction with glucose, amino acids and potassium on net metabolism of glucose and amino acids in the goat mammary gland

Published online by Cambridge University Press:  01 June 2009

Sophie Tesseraud ,
Jean Grizard ,
Boguslaw Makarski ,
Elisabeth Debras ,
Gerard Bayle  and
Claude Champredon
Sophie Tesseraud
Affiliation:
Laboratoire d'Étude du Métabolisme Azoté, INRA, Centre de Clermont-Ferrand Theix, 63122 Ceyrat, France
Jean Grizard
Affiliation:
Laboratoire d'Étude du Métabolisme Azoté, INRA, Centre de Clermont-Ferrand Theix, 63122 Ceyrat, France
Boguslaw Makarski
Affiliation:
Laboratoire d'Étude du Métabolisme Azoté, INRA, Centre de Clermont-Ferrand Theix, 63122 Ceyrat, France
Elisabeth Debras
Affiliation:
Laboratoire d'Étude du Métabolisme Azoté, INRA, Centre de Clermont-Ferrand Theix, 63122 Ceyrat, France
Gerard Bayle
Affiliation:
Laboratoire d'Étude du Métabolisme Azoté, INRA, Centre de Clermont-Ferrand Theix, 63122 Ceyrat, France
Claude Champredon
Affiliation:
Laboratoire d'Étude du Métabolisme Azoté, INRA, Centre de Clermont-Ferrand Theix, 63122 Ceyrat, France
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Summary

The hyperinsulinaemic euglycaemic insulin clamp technique was used to study the effect of insulin on the arterio-venous concentration differences of glucose and amino acids across the mammary gland in dairy goats. Insulin was given in conjunction with K to prevent insulin hypokalaemia. Appropriate amino acid infusion was used to blunt insulin-induced hypoaminoacidaemia or to create hyperaminoacidaemia and maintain this state under insulin treatment. Hyper-aminoacidaemia alone only stimulated mammary leucine uptake but did not significantly modify the net metabolism of other amino acids and glucose. Insulin infusion at physiological level in conjunction with glucose, KCl-NaCl and amino acids failed to alter mammary uptake of glucose and essential amino acids; occasional increase in arginine extraction and decrease in tyrosine extraction were exceptions. Thus these new experimental conditions did not reveal any galactopoietic effect of insulin.

Type
Original Articles
Information
Journal of Dairy Research , Volume 59 , Issue 2 , May 1992 , pp. 135 - 149
Copyright
Copyright © Proprietors of Journal of Dairy Research 1992

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