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Effect of heat stress on the interaction of Streptococcus uberis with bovine mammary epithelial cells

Published online by Cambridge University Press:  22 February 2018

Raúl A. Almeida ,
Oudessa Kerro-Dego  and
Agustín G. Rius
Raúl A. Almeida*
Affiliation:
Department of Animal Science, The University of Tennessee, Knoxville, TN, USA
Oudessa Kerro-Dego
Affiliation:
Department of Animal Science, The University of Tennessee, Knoxville, TN, USA
Agustín G. Rius
Affiliation:
Department of Animal Science, The University of Tennessee, Knoxville, TN, USA
*
*For correspondence; e-mail: [email protected]
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Abstract

Heat stress (HS) negatively affects milk production and has been associated with decreased immune function, and increased rate of intramammary infections (IMI). Research has shown that HS affects gene expression, cell cycle, and cell metabolism in bovine mammary epithelial cells (BMEC). Since BMEC are an initial target of mastitis pathogens, we studied adherence to and internalisation of S. uberis into HS-BMEC, as well as the effect that this interaction has on host cells by measuring HS-BMEC viability and membrane integrity. Results reported in this Research Communication showed that HS reduced cell viability and induced membrane damage. However, these pathological changes, as well as the rate of adherence and internalisation of S. uberis into BMEC, were augmented when S. uberis was cocultured with HS-BMEC. These results may help to understand the pathogenesis of S. uberis IMI as well as the increased susceptibility of mammary glands to IMI in cows subjected to HS.

Keywords

Streptococcus uberisbovine mammary epithelial cellsheat stresspathogenesis
Type
Research Article
Information
Journal of Dairy Research , Volume 85 , Issue 1 , February 2018 , pp. 53 - 56
Copyright
Copyright © Hannah Dairy Research Foundation 2018 

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